Ultrastructural changes in skeletal muscles in dengue virus‐infected mice

Nath, Pramod; Agrawal, Devendra K.; Mehrotra, R. M. L.

doi:10.1002/path.1711360405

Cited by 21 publications

(20 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Electron microscopic studies conducted on the striated muscles of dengue infected mice showed destruction of myofibrils, rarification of the sarcoplasmic reticulum network, and changes in the mitochondria. In addition, aggregates of the electron-dense material as well as the glycogen particles were also seen in the cytoplasm [16]. Histopathology revealed the presence of inflammatory cells and fibrotic areas in one of the patients on whom muscle biopsy was performed.…”

Section: Discussionmentioning

confidence: 95%

Acute dengue virus myositis: A report of seven patients of varying clinical severity including two cases with severe fulminant myositis

Paliwal

Garg

Juyal

et al. 2011

Journal of the Neurological Sciences

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 95%

Acute dengue virus myositis: A report of seven patients of varying clinical severity including two cases with severe fulminant myositis

Paliwal

Garg

Juyal

et al. 2011

Journal of the Neurological Sciences

View full text Add to dashboard Cite

“…Previous studies of DENV infection of muscle cells have used mouse models to identify and document biochemical and structural changes in skeletal muscle during acute DENV infection (Agrawal et al, 1978;Nath et al, 1982). However, mice are not a natural host for DENV (Gubler, 1994).…”

Section: Discussionmentioning

confidence: 99%

“…However, two clinical studies have reported myositis during acute DENV infection (Kalita et al, 2005;Rajajee et al, 2005). Previous studies of DENV infection in mice have reported biochemical and ultrastructural changes in skeletal muscle (Nath et al, 1982;Rajajee et al, 2005), as well as the detection of infectious DENV progeny in the muscles (Agrawal et al, 1978). Fatal myocarditis has also been observed during acute DENV infection (I. Villalobos & J.…”

Section: Introductionmentioning

confidence: 99%

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Warke

Becerra

Zawadzka³

et al. 2008

Journal of General Virology

View full text Add to dashboard Cite

Dengue virus (DENV) is a mosquito-borne flavivirus that causes an acute febrile disease in humans, characterized by musculoskeletal pain, headache, rash and leukopenia. The cause of myalgia during DENV infection is still unknown. To determine whether DENV can infect primary muscle cells, human muscle satellite cells were exposed to DENV in vitro. The results demonstrated for the first time high-efficiency infection and replication of DENV in human primary muscle satellite cells. Changes in global gene expression were also examined in these cells following DENV infection using Affymetrix GeneChip analysis. The differentially regulated genes belonged to two main functional categories: cell growth and development, and antiviral type I interferon (IFN) response genes. Increased expression of the type I IFN response genes for tumour necrosis factor-related apoptosis-inducing ligand (TRAIL), melanoma-derived antigen 5 (MDA-5), IFN-c-inducible protein 10 (IP-10), galectin 3 soluble binding protein (LGals3BP) and IFN response factor 7 (IRF7) was confirmed by quantitative RT-PCR. Furthermore, higher levels of cell-surface-bound intracellular adhesion molecule-1 (ICAM-1) and soluble ICAM-1 in the cellculture medium were detected following DENV infection. However, DENV infection impaired the ability of the infected cells in the culture medium to upregulate cell-surface expression of MHC I molecules, suggesting a possible mechanism of immune evasion by DENV. The findings of this study warrant further clinical research to identify whether muscle cells are targets for DENV infection during the acute stage of the disease in vivo. INTRODUCTIONDengue is an increasingly troublesome, mosquito-borne viral disease that is highly prevalent in both tropical and subtropical regions of the world. Annually, 50-100 million cases of dengue fever (DF) and 250 000 cases of dengue haemorrhagic fever (DHF) occur worldwide, as determined by the World Health Organization (Nathan & DayalDrager, 2006;Petersen & Marfin, 2005). Infection with any one of the four serotypes of DENV (DENV1-4) can cause DF or DHF. The general mechanisms that control virus replication and are involved in disease pathogenesis are not well understood in this complex, acute illness.Monocytes, dendritic cells and B cells are known to be susceptible to DENV in vivo (Halstead, 1989;King et al., 1999;Lin et al., 2002;Marovich et al., 2001;Wu et al., 2000), whilst DENV antigen has been detected in sinusoidal endothelial cells (Jessie et al., 2004) and hepatocytes (de Macedo et al., 2006;Rosen et al., 1999) in autopsy studies. In addition, a variety of primary human cells, including mast cells (King et al., 2002), endothelial et al., 2007) and hepatocytes (Suksanpaisan et al., 2007), can serve as hosts for DENV in vitro.Symptomatically, patients with dengue often present with general muscle affection as well as severe, persisting myalgia, headache and rash (Chaturvedi et al., 1970;Halstead, 1966). Higher serum levels of creatine phosphokinase (CPK), which is specifically produ...

show abstract

“…Different mechanisms have been hypothesized, such as direct viral invasion of and immune-mediated injury to the muscle fibers. Striated skeletal muscles from mice inoculated with dengue type 2 viruses exhibited myofibril destruction, sarcoplasm involution, mitochondrial changes and aggregates of electron-dense material and cytoplasmic glycogen particles 32 . The dengue virus has demonstrated high efficiency in infecting and replicating in human primary muscle satellite cells 38 and muscle cells were demonstrated to be highly susceptible to in vitro infection by the dengue 2 virus.…”

Section: Discussionmentioning

confidence: 98%

“…Hemodynamic instability, hemolysis, glomerular injury and direct action of viral particles on renal tissue have been considered as possible injury mechanisms 6,10,25,30,32,34,37 . In fact, the current patient presented proteinuria but the renal biopsy did not disclose glomerular injury.…”

Section: Discussionmentioning

confidence: 99%

Biopsy Proven Acute Tubular Necrosis Due to Rhabdomyolysis in a Dengue Fever Patient: A Case Report and Review of Literature

Repizo

Malheiros

et al. 2014

Rev. Inst. Med. trop. S. Paulo

View full text Add to dashboard Cite

Renal histology results are very scarce in dengue-associated rhabdomyolysis patients developing acute kidney injury (AKI). We report a case of dengue fever-induced AKI associated to rhabdomyolysis with a renal biopsy showing acute tubular necrosis (ATN) and renal deposition of myoglobin. A 28-year-old patient who presented dengue fever (DF) complicated by severe AKI and rhabdomyolysis is described. The patient required hemodialysis for three weeks. A renal biopsy revealed ATN with positive staining for myoglobin in the renal tubuli. The patient was discharged with recovered renal function. In conclusion, this case report described a biopsy proven ATN associated to DF-induced rhabdomyolysis, in which renal deposition of myoglobin was demonstrated. We suggest that serum creatine phosphokinase should be monitored in DF patients to allow for an early diagnosis of rhabdomyolysis and the institution of renal protective measures.

show abstract

Ultrastructural changes in skeletal muscles in dengue virus‐infected mice

Cited by 21 publications

References 9 publications

Acute dengue virus myositis: A report of seven patients of varying clinical severity including two cases with severe fulminant myositis

Acute dengue virus myositis: A report of seven patients of varying clinical severity including two cases with severe fulminant myositis

Efficient dengue virus (DENV) infection of human muscle satellite cells upregulates type I interferon response genes and differentially modulates MHC I expression on bystander and DENV-infected cells

Biopsy Proven Acute Tubular Necrosis Due to Rhabdomyolysis in a Dengue Fever Patient: A Case Report and Review of Literature

Contact Info

Product

Resources

About