Ultrastructural changes associated with carbon tetrachloride hepatotoxicity in channel catfish, Ictalurus punctatus Rafinesque

Abstract

Ultrastructural changes occurring in juvenile ehannel eattish, Ietalurus punetatus Ratinesque. hepatoeytes were examined following exposure to carbon tetrachloride (CCI4). Juvenile cattish were intraperitoneally injected with a sublethal dose of undiluted CCU and controls, used for comparisons, received physiological saline. The rough endoplasmic reticulum (RER) was slightly dilated by 1 h and was significantly dilated by 4 and 24h post-CCU-exposure. At 4h. the RER became degranulated, mitoehondrial eristae indistinet with electron dense floeculant matrieal deposits, and nuclear membranes indented. Autophagic vacuoles were present in some hepatocytes. Within 48 h post-exposure, the hepatocytes were indistinguishable from control hepatoeytes. CCI4 ean induee reversible or irreversible cell damage which appears dose dependent.

Ultrastructural Changes Associated With Molluscicide Hepatotoxicity in Fishes

“…The prominent leached fat globules showed evidence of mobilization and utilization of stored lipid in liver of Gambusia affinis, which may have been resulted from hepatotoxicity induced by Bayluscide causing abnormal metabolic situation, by MFOs in RER of hepatocytes (Stegeman & Hahn, 1993). This is in agreement with Afifi & Mac Millan (1992) who suggested that direct solvent action (according to Berger et al" 1987) was not responsible for the changes observed. They recorded that the ultrastructural changes, observed in parenchymal hepatocyte mitochondria and other organelles, indicated that initial alterations occurred to the RER, and concluded that the lipid peroxidation accounted for the ultrastructural alterations that were detected (according to Comporti, 1985 andCosta &Trudell, 1989).…”

“…In severely affecting hepatocytes, there was parenchymal loss, the vacuoles occupied most of the cytoplasm and intercellular membranes were either broken or became indistinguishable. Similar alterations were observed in the liver of Ictalurus punctatus (Afifi & Mac Millan, 1992)'and Clarias lazera (Yacoub, 1999).…”