Ultrastructural analyses of the novel chimeric hemostatic agent generated via nanotechnology, ABS nanohemostat, at the renal tissue level

Abstract

Ankaferd Blood Stopper (ABS), a hemostatic agent of plant origin, has been registered for the prevention of clinical hemorrhages. Currently there is no data regarding the ultrastructural analysis of ABS at the tissue level. The aim of this study is to assess renal tissue effects via scanning electron microscopy (SEM) analyses for the ABS and ABS nanohemostat (formed by the combination of self-assembling peptide amphiphile molecules and ABS). SEM experiments were performed with FEI Nova NanoSEM 230, using the ETD detector at low vacuum mode with 30 keV beam energy. SEM analyses revealed that significant erythroid aggregation are present inside the capillary bed of the renal tissue. However, neither the signs of necrosis nor any other sign of tissue damage are evident in the surrounding renal tissue supplied by the microcapillary vasculature. Our study is important for several reasons. Firstly, in our study we used ABS nanohemostat which was recently developed. This study adds valuable information to the literature regarding ABS nanohemostat. Secondly, this study is the first ultrastructural analysis of ABS that was performed at the tissue level. Thirdly, we disclosed that ABS nanohemostat could induce vital erythroid aggregation at the renal tissue level as detected by SEM. Lastly, we detected that ABS nanohemostat causes no harm to the tissues including necrosis and any other detrimental effects.

Differentiation of Tarchonanthus camphoratus L. and Tarchonanthus parvicapitulatus P.P.J. Herman (Asteraceae) using electron microscopy, and comparison of their biological activities

“…Studies conducted on the pollen morphology of the T. camphoratus complex using Scanning Electron Microscopy (SEM) and Transmission Electron Microscopy (TEM) also confirmed that there are different taxa grouped together (Zavada and Lowrey, 2010). The use of Scanning Electron Microscopy to study the micromorphology of eukaryotes including plants has proven useful as a tool to resolve many taxonomic uncertainties (Huri et al, 2016). Therefore, micromorphological studies were more useful in distinguishing between the two species than the macromorphological study.…”

Advances in the development and optimization strategies of the hemostatic biomaterials

“…SLac loaded with bactretase produced a kind of drug-loaded hydrogel named SB50, which enhanced the role of coagulation. Huri, Dogantekin et al (2016) mixed ABS hemostatic agent with amphiphilic short peptide (Lauryl -VVAGK-Am) 1:1 to obtain a kind of nano hemostatic agent, which played an excellent hemostatic role in partial nephrectomy.…”