Ultrasound microbubble-mediated transfection of NF-κB decoy oligodeoxynucleotide into gingival tissues inhibits periodontitis in rats in vivo

Abstract

Periodontitis is a chronic infectious disease for which the fundamental treatment is to reduce the load of subgingival pathogenic bacteria by debridement. However, previous investigators attempted to implement a nuclear factor kappa B (NF-κB) decoy oligodeoxynucleotide (ODN) as a suppressor of periodontitis progression. Although we recently reported the effectiveness of the ultrasound-microbubble method as a tool for transfecting the NF-κB decoy ODN into healthy rodent gingival tissue, this technique has not yet been applied to the pathological gingiva of periodontitis animal models. Therefore, the aim of this study was to investigate the effectiveness of the technique in transfecting the NF-κB decoy ODN into rats with ligature-induced periodontitis. Micro computed tomography (micro-CT) analysis demonstrated a significant reduction in alveolar bone loss following treatment with the NF-κB decoy ODN in the experimental group. RT-PCR showed that NF-κB decoy ODN treatment resulted in significantly reduced expression of inflammatory cytokine transcripts within rat gingival tissues. Thus, we established a transcutaneous transfection model of NF-κB decoy ODN treatment of periodontal tissues using the ultrasound-microbubble technique. Our findings suggest that the NF-κB decoy ODN could be used as a significant suppressor of gingival inflammation and periodontal disease progression.

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

“…The ultrasound intensity could be easily controlled, making it easy to break microbubbles and release target genes at the designated time and place [ 20 ]. Also, microbubbles could travel through lung and respiratory system and have fewer side effects [ 11 ]. Moreover, as compared with liposome, the transfection efficiency has been proven to have better effects [ 21 ].…”

“…Agents for microbubble ultrasound contrast emerge as novel vectors for gene transfection, as they showed high safety, stability, and efficiency [ 10 ]. Microbubbles ‘break’ and release target gene under the energy from ultrasound radiation, which can increase cell permeability and create a reversible sound hole that promotes the infiltration of target genes into the nucleus and therefore increase gene transfection and expression efficiency [ 11 , 12 ]. In clinical practice, applying microbubble ultrasound agent in gene therapy has attracted much attention due to its high efficiency of transfection and low side effects [ 13 ].…”

<p>The better effects of microbubble ultrasound transfection of miR-940 on cell proliferation inhibition and apoptosis promotion in human cervical cancer cells</p>

“…First, for the ultrasound could be controlled in time and space, it can break microbubble to release target genes at high concentrations in special place, realizing the targeting characteristic 32. Second, for the gas of microbubble are steady, and the short diameter and long half-life period, they can go through lung and respiratory system to be excreted to the outside of the body, therefore possessing low side effect 26. Third, the transfection efficiency is proved better than liposome 33.…”

“…Microbubble ultrasound contrast agent is a new gene transfer vector of safe, stable, and efficient characteristics 25. The microbubble can “break” under the energy of ultrasound radiation, releasing the target gene on it 26. The vibration of microbubble destruction could increase the permeability of local cells and produce a reversible sound-hole, promoting target genes into cell nucleus to increase the efficiency of gene transfection and expression 27.…”

High-fluence low-power laser irradiation promotes odontogenesis and inflammation resolution in periodontitis by enhancing stem cell proliferation and differentiation

“…All rats were fed with the same food (at 8:00 a.m. and 8:00 p.m.). Rats in the HF-LPLI groups underwent surgery, followed by a 1-week 20-J/cm 2 HF-LPLI treatment for 1 h/day after local injection of 1×10 5 /ml stem cells in 100 μ l, followed by the in vivo bioluminescence imaging and H&E staining ( 42 , 43 ). The Height of bone regeneration was calculated as 8 mm−(a1 + a2 + a3)/3.…”