Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis
Abstract:This study compared the effects of ultrasonic irradiation and SonoVue microbubbles (US) or Lipofectamine 3000 on the transfection of small interfering RNA for PRR11 (siPRR11) and PRR11 overexpression plasmid into breast cancer cells. SiPRR11 and PRR11 overexpression plasmid were transfected into breast cancer MCF7 cells mediated by US and Lipofectamine 3000. PRR11 expressions in breast cancer and normal tissues were determined using Gene Expression Profiling Interactive Analysis (GEPIA). The viability, prolife… Show more
“…Other studies highlighted that UTMD of miR-133a could inhibit the tumor growth and improve the survival rate in breast cancer mice [ 38 ], and UTMD of Cav1.3 siRNA exerted antitumor effects on breast cancer [ 39 ]. In addition to the aforementioned literature’s illustration of the feasibility of UTMD as a gene transfection modality to treat breast cancer, another study by Hui Luo et al evidenced that silenced PRR11 mediated by US exhibited a higher efficiency than that mediated by lipofectamine 3000 [ 40 ]. Besides, Xiaojiang Tang et al proved that the inhibitory effect of UTMD-mediated SOCS3 on the biological behavior of breast cancer cells was better than that of liposome-mediated SOCS3 [ 41 ].…”
In the context of relatively sufficient research that annotated WNT1 inducible signaling pathway protein 1 (WISP1) as a promoting factor in tumor progression of breast cancer, and identified the effects of ultrasound microbubble technology on enhancing the transfection efficiency and achieving better gene interference, this study managed to investigate the effects of ultrasound microbubble-mediated siWISP1 transfection on proliferation and metastasis of breast cancer cells. To achieve our research objectives, the expression of WISP1 in breast cancer tissues was retrieved from GEPIA website, and the viability of breast cancer cells (SK-BR-3 and MCF7) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for ultrasound intensity screening. After the transfection of siWISP1 by ultrasound microbubble or lipofectamine 6000, the content of WISP1 secreted by cells was detected through Enzyme-linked immunosorbent assay (ELISA), and WISP1 expression in cells was determined by quantitative reverse transcription polymerase-chain reaction (qRT-PCR). Besides, the cell invasion, migration, and proliferation were evaluated by wound healing, transwell, and EdU assays, respectively. In accordance with experimental results, WISP1 was highly expressed in breast cancer tissues, and the 1 W/cm
2
intensity was the onset of a notable decrease in cell viability. Compared with lipofectamine 6000 transfection, the transfection of siWISP1 mediated by ultrasound microbubble further reduced the expression of WISP1, and meanwhile suppressed cell invasion, migration, and proliferation. Collectively, ultrasound microbubble-mediated transfection of siWISP1 worked rather effectively in improving transfection efficiency and inhibiting the progression of breast cancer.
“…Other studies highlighted that UTMD of miR-133a could inhibit the tumor growth and improve the survival rate in breast cancer mice [ 38 ], and UTMD of Cav1.3 siRNA exerted antitumor effects on breast cancer [ 39 ]. In addition to the aforementioned literature’s illustration of the feasibility of UTMD as a gene transfection modality to treat breast cancer, another study by Hui Luo et al evidenced that silenced PRR11 mediated by US exhibited a higher efficiency than that mediated by lipofectamine 3000 [ 40 ]. Besides, Xiaojiang Tang et al proved that the inhibitory effect of UTMD-mediated SOCS3 on the biological behavior of breast cancer cells was better than that of liposome-mediated SOCS3 [ 41 ].…”
In the context of relatively sufficient research that annotated WNT1 inducible signaling pathway protein 1 (WISP1) as a promoting factor in tumor progression of breast cancer, and identified the effects of ultrasound microbubble technology on enhancing the transfection efficiency and achieving better gene interference, this study managed to investigate the effects of ultrasound microbubble-mediated siWISP1 transfection on proliferation and metastasis of breast cancer cells. To achieve our research objectives, the expression of WISP1 in breast cancer tissues was retrieved from GEPIA website, and the viability of breast cancer cells (SK-BR-3 and MCF7) was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay for ultrasound intensity screening. After the transfection of siWISP1 by ultrasound microbubble or lipofectamine 6000, the content of WISP1 secreted by cells was detected through Enzyme-linked immunosorbent assay (ELISA), and WISP1 expression in cells was determined by quantitative reverse transcription polymerase-chain reaction (qRT-PCR). Besides, the cell invasion, migration, and proliferation were evaluated by wound healing, transwell, and EdU assays, respectively. In accordance with experimental results, WISP1 was highly expressed in breast cancer tissues, and the 1 W/cm
2
intensity was the onset of a notable decrease in cell viability. Compared with lipofectamine 6000 transfection, the transfection of siWISP1 mediated by ultrasound microbubble further reduced the expression of WISP1, and meanwhile suppressed cell invasion, migration, and proliferation. Collectively, ultrasound microbubble-mediated transfection of siWISP1 worked rather effectively in improving transfection efficiency and inhibiting the progression of breast cancer.
“…Moreover, PRR11 blocked p85 homodimerization and sensitized to ligand-induced PI3K activation, suggesting that PRR11 amplification confers resistance to estrogen deprivation through hyperactivation of the PI3K pathway [ 1 ]. Notably, ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 could exert anti-cancer effects via PRR11 [ 41 ]. However, the oncogenic role of PRR11 in vivo is still blurred, demanding additional exploration.…”
Section: Oncogenic Role Of Prr11 In Various Malignanciesmentioning
confidence: 99%
“…Preliminary experiments found that SonoVue microbubbles-mediated RNA interference targeting PRR11 could exert anti-cancer effects, forecasting the potential application of PRR11 for targeted therapy [ 9 , 41 ]. In the meanwhile, circRNA_cZNF292 was involved in angiogenesis of glioma through PRR11 [ 77 ].…”
Section: Prr11 Involved Targeted Therapies In Various Malignanciesmentioning
confidence: 99%
“…Chalcone, modificated by dithiocarbamate scaffolds, exert inhibitory effects via PRR11 downregulation [ 30 ]. Furthermore, PRR11 was involved in the treatment of ultrasonic irradiation for breast cancer [ 41 ]. Therefore, targeting PRR11 might provide a reliable and promising therapy for human malignancies ( Figure 3 ).…”
Section: Prr11 Involved Targeted Therapies In Various Malignanciesmentioning
Proline rich 11 (PRR11), initially renowned for its relevance with cell-cycle progression, is a proline-rich protein coding gene in chromosome 17q22-23. Currently, accumulating studies have demonstrated that PRR11 plays a critical role in cellular proliferation, colony formation, migration, invasion, cell-cycle progression, apoptosis, autophagy and chemotherapy resistance via multiple signaling pathways and biological molecules in several solid tumors. In particular, PRR11 also serves as a promising prognostic indicator in a limited number of human cancers, gradually manifesting its potential application for targeted therapies. In this review, we summarize functional activities, related signaling pathways and biological molecules of PRR11 in various malignancies and generalize potential application of PRR11 for targeted therapies, thereby contributing to further exploration of PRR11 in cancer treatment.
“…PRR11 is highly expressed in many human cancers and has been regarded as a potential biomarker for poor prognosis 9–14 . Moreover, numerous studies have shown that PRR11 participates in the tumorigenesis and development of many cancers, such as lung cancer, hepatocellular carcinoma, osteosarcoma, clear cell renal cell carcinoma, breast cancer, tongue squamous cell carcinoma (TSCC), esophageal squamous cell carcinoma, and pancreatic cancer 15–22 …”
Cutaneous squamous cell carcinoma (cSCC) is one of the most common skin malignancies, and its incidence rate is increasing worldwide. Proline-rich 11 (PRR11) has been reported to be involved in the occurrence and development of various tumors. However, the role of PRR11 in cSCC remains unknown. In the present study, we observed upregulated expression of PRR11 in cSCC tissues and cell lines. Knockdown of PRR11 in the cSCC cell lines A431 and SCL-1 inhibited cell proliferation by inducing cell cycle arrest during the G1/S phase transition, promoted cell apoptosis, and reduced cell migration and invasion in vitro. Conversely, overexpression of PRR11 promoted cell proliferation, decreased cell apoptosis, and enhanced cell migration and invasion. PRR11 knockdown also inhibited cSCC tumor growth in a mouse xenograft model.Mechanistic investigations by RNA sequencing revealed that 891 genes were differentially expressed genes between cells with PRR11 knockdown and control cells. Enrichment analysis of different genes showed that the epidermal growth factor receptor (EGFR) signaling pathway was the top enriched pathway. We further validated that PRR11 induced EGFR pathway activity, which contributed to cSCC progression. These data suggest that PRR11 may serve as a novel therapeutic target in cSCC.
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