<p>The better effects of microbubble ultrasound transfection of miR-940 on cell proliferation inhibition and apoptosis promotion in human cervical cancer cells</p>

Abstract

PurposeCervical cancer is the second leading cause of women’s cancer-related death. MiR-940 has been reported as a critical factor in various cancers. Based on the high transfection efficiency and low side effect, the clinical application of microbubble ultrasound contrast agent in gene treatment has attracted a widespread attention. In this study, we determined the mechanism of miR-940 inhibiting cell proliferation and cycle procession, and promoting cell apoptosis in cervical cancer Hela cells. In addition, we compared the effects of different transfection methods, including liposome, microbubble, ultrasound, and microbubble coupled with ultrasound.Patients and methodsMTT assay, PI staining, and Annexin-Ⅴ/PI staining assays were, respectively, performed to evaluate cell proliferation status, cell cycle progression, and apoptosis status. RT-PCR and Western blot were conducted to measure the levels of cell cycle- and apoptosis-related factors, and the phosphorylation levels of PI3K and Akt.ResultsResults showed that the overexpression of miR-940 inhibited cell proliferation, blocked cell cycle, and promoted apoptosis by regulating cell cycle-related factors (such as inhibited Cyclin D1 and CDK4) and apoptosis-related factors (such as promoted Puma and Bax, inhibited Bcl-2 and Cleaved caspase9), and inhibiting the phosphorylation and activation of PI3K/AKT pathway. Among all of them, miR-940 transfected with microbubble and ultrasound showed the greatest changes.ConclusionIt provides evidence that miR-940 could be a wonderful biomarker and treatment agent for cervical cancer, and microbubble ultrasound would have more wide application in the clinical treatment of cancers.

Ultrasound Microbubbles-mediated Microrna-505 Regulates Cervical Cancer Cell Growth via AKT2

“…In Ca-Ski and HeLa cells, miR-505 upregulation suppressed proliferation and tumorigenicity, indicating miR-505 may act as an inhibitor in CC [30]. Similarly, compared with liposome, MBs or ultrasound transfection, USMB-miR-940 further inhibited CC malignant episodes [31]. Our study may offer a novel approach for CC treatment from the miR-505 delivery by USMB treatment.…”

“…Consistently, miR-505 was poorly expressed in CC, and negatively correlated with tumor histology grade and lymph node metastasis [25,30]. Additionally, miR-940 inhibited CC cell proliferation, and USMB-miR-940 showed better effects [31]. However, the role of USMB treatment on miR-505 expression was not yet studied.…”

Ultrasonic irradiation and SonoVue microbubbles-mediated RNA interference targeting PRR11 inhibits breast cancer cells proliferation and metastasis, but promotes apoptosis

“…Regulation of the proliferation of cancer cells is often achieved with the modulation on the critical factors, such as Bcl-2 and Bax, which are two cell apoptosis-related genes [ 28 ]. Bcl-2 could suppress cell apoptosis either through preventing the release of cytochrome c from mitochondria or through binding with the apoptosis-activating factor (APAF-1) [ 29 ].…”

Ultrasound Microbubble-Mediated microRNA-505 Regulates Cervical Cancer Cell Growth via AKT2

“…miR-505 was poorly expressed in CC and negatively correlated with tumor histology grade and lymph node metastasis [ 25 , 30 ]. Additionally, miR-940 inhibited CC cell proliferation, and USMB-miR-940 showed better effects [ 31 ]. However, the role of the USMB treatment on miR-505 expression was not yet studied.…”