Ultrasound Microbubbles-mediated Microrna-505 Regulates Cervical Cancer Cell Growth via AKT2

Abstract

Background: The gene-loaded microbubbles (MBs) combined with ultrasound resulting in increased delivery efficiency, may be a novel method of gene delivery. We explored the effects of ultrasound and microbubbles (USMB)-mediated microRNA (miR)-505 on cervical cancer (CC) development.Methods: miR-505 mediated by USMB was prepared. The effect of miR-505 on its transfection efficiency was studied by RT-qPCR. The effect of miR-505 on HeLa cell proliferation was evaluated by MTT and colony formation assays. Flow cytometry was used to study cell cycle changes, Hoechst was utilized to detect apoptosis. Through the wound healing and Transwell assay, the migration and invasion ability of HeLa cells were measured. The target gene of miR-505 was predicted, and its expression in CC was detected. The target relationship and the effect of the target gene on HeLa cells were further verified.Results: USMB-miR-505 showed higher transfection efficiency than miR-505 alone. miR-505 inhibited HeLa cell malignant episodes, which were reinforced by USMB treatment. miR-505 targeted AKT2. AKT2 was highly expressed in CC, and overexpression of AKT2 significantly reversed the inhibitory effect of miR-505 mediated by USMB on HeLa cell malignant biological behaviors.Conclusion: USMB-miR-505 inhibited HeLa cell malignant biological behaviors by targeting AKT2.