Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Wu, Xun; Qin, Kele; Wang, Di; Xiang, Kun; Peng, Jun; Guo, Jianjun; Yang, Jun; Fan, Chengming

doi:10.3791/63647

Cited by 3 publications

(3 citation statements)

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“…Ultrasound‐guided injection into other blood vessels and organs for the administration of various cell types in preclinical studies are well reported. 1 , 2 , 23 The requirement for a 27G needle to allow for the islets to be transplanted is, in this scenario, the main confounding factor as to why this method is not working adequately, and results in the bevel of the needle not fully entering the HPV for reliable dosing in the mouse.…”

Section: Discussionmentioning

confidence: 99%

Ultrasound‐guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice

et al. 2023

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BackgroundOpen laparotomy hepatic portal vein injection is a well‐used pre‐clinical technique for transplantation of cell therapies to the liver in mice, including islet transplantation studies for type 1 diabetes. We hypothesised that ultrasound‐guided hepatic portal vein injection in mice would provide reproducible and reliable results, as is currently obtained via open laparotomy techniques, and offer a surgical refinement to emulate islet transplantation in humans.MethodsFluorescent‐polymer microparticles (20 μm) were injected (27G‐needle) into the hepatic portal vein via open laparotomy (n=4) or under ultrasound‐guidance (n=4) using an MX550D‐transducer with a Vevo3100‐scanner (FUJIFILM VisualSonics, Inc., Canada). Mice were culled 24‐hours post injection; organs were frozen, step sectioned (10 μm‐slices,) and 10 sections/mouse (50 μm‐spacing) were quantified for microparticles in the liver and other organs by fluorescent microscopy.ResultsMurine hepatic portal vein injection, via open laparotomy‐route, resulted in widespread distribution of microparticles in the liver, lungs and spleen; ultrasound‐guided injection resulted in reduced microparticle delivery (p<0.0001) and microparticle clustering in distinct areas of the liver at the site of needle penetration, with very few/no microparticles being seen in lung and spleen tissues, hypothesised to be due to flow into the body cavity: liver median(IQR) 4.15(0.00‐4.15) versus 0.00(0.00‐0.00) particle‐count mm‐2, respectively.ConclusionsUltrasound‐guided injection results in microparticle clustering in the liver, with an overall reduction in microparticle number when compared to open laparotomy hepatic portal vein injection, and high variability in microparticle‐counts detected between mice. Ultrasound‐guided injection is not currently a technique that can replace open laparotomy hepatic portal vein injection of islet transplantation in mice.

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Section: Discussionmentioning

confidence: 99%

Ultrasound‐guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice

et al. 2023

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“…To solve the problem of the low heart residence rate of transplanted cells, researchers are using assistive devices to increase the rate of cell implantation. Under the guidance of echocardiography, via xiphisternum under oblique into the left ventricular myocardial injection hiPSC-CMs, the method of cell transplantation ratio increase greatly, by evaluating around mouse MI cardiac function after transplantation of apoptosis, angiogenesis, cell size, can be observed by ultrasound-guided cell transplantation group get considerable heart repair ability, this treatment has shown feasibility and effectiveness in previous studies (Wu et al, 2022). In addition, engineered tissues of different geometric shapes and sizes are manufactured to meet exogenous structural and electromechanical maturity and promote the development of physiological tissue structures.…”

Section: Tissue Engineering Technologymentioning

confidence: 99%

“…In addition, the dose and frequency of cell injection still need to be considered. Studies have shown that only one‐tenth of the cells survived by injecting hiPSC‐CMs into the MI area and the MI junction area to improve the microenvironment at the MI site, while the number of cells survived by injecting hiPSC‐CMS three times was 10 times that of one injection (Wu et al, 2022). Single‐cell/nuclear sequencing results of human fetal and adult hearts revealed that the same type of cells in different cardiac sites have their heterogeneity and molecular properties.…”

Section: Cell Therapy For Heart Regenerationmentioning

confidence: 99%

The origin, progress, and application of cell‐based cardiac regeneration therapy

Zhuo

Lei

et al. 2023

Journal Cellular Physiology

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Cardiovascular disease (CVD) has become a severe threat to human health, with morbidity and mortality increasing yearly and gradually becoming younger. When the disease progresses to the middle and late stages, the loss of a large number of cardiomyocytes is irreparable to the body itself, and clinical drug therapy and mechanical support therapy cannot reverse the development of the disease. To explore the source of regenerated myocardium in model animals with the ability of heart regeneration through lineage tracing and other methods, and develop a new alternative therapy for CVDs, namely cell therapy. It directly compensates for cardiomyocyte proliferation through adult stem cell differentiation or cell reprogramming, which indirectly promotes cardiomyocyte proliferation through non‐cardiomyocyte paracrine, to play a role in heart repair and regeneration. This review comprehensively summarizes the origin of newly generated cardiomyocytes, the research progress of cardiac regeneration based on cell therapy, the opportunity and development of cardiac regeneration in the context of bioengineering, and the clinical application of cell therapy in ischemic diseases.

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Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering

Tayanloo-Beik

Nikkhah²,

Roudsari³

et al. 2022

Advances in Experimental Medicine and Biology

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Ultrasound-Guided Induced Pluripotent Stem Cell-Derived Cardiomyocyte Implantation in Myocardial Infarcted Mice

Cited by 3 publications

References 0 publications

Ultrasound‐guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice

Ultrasound‐guided hepatic portal vein injection is not a reproducible technique for delivery of cell therapies to the liver in mice

The origin, progress, and application of cell‐based cardiac regeneration therapy

Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering

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