Application of Biocompatible Scaffolds in Stem-Cell-Based Dental Tissue Engineering

Tayanloo-Beik, Akram; Nikkhah, Amirabbas; Roudsari, Peyvand Parhizkar; Aghayan, Hamid Reza; Rezaei‐Tavirani, Mostafa; Nasli‐Esfahani, Ensieh; Mafi, Ahmad R.; Nikandish, Mohsen; Shouroki, Fatemeh Fazeli; Arjmand, Babak; Larijani, Bagher

doi:10.1007/5584_2022_734

Cited by 2 publications

(1 citation statement)

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“…Ideally, cells that are administered for dental pulp regeneration purposes would support dentin synthesis, enable an efficient dentin-pulp/soft tissue interaction, withstand hypoxia, facilitate anti-inflammatory and regenerative molecular signaling pathways, while building a hard tissue barrier towards potentially inserted repair materials [ 14 , 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. In the light of their proliferative capacity, their developmental potential, as well as their self-regenerative properties, SCs are optimal candidates for such applications.…”

Section: Introductionmentioning

confidence: 99%

Characterization of a Stemness-Optimized Purification Method for Human Dental-Pulp Stem Cells: An Approach to Standardization

Dieterle

Gross

Steinberg

et al. 2022

Cells

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Human dental pulp stem cells (hDPSCs) are promising for oral/craniofacial regeneration, but their purification and characterization is not yet standardized. hDPSCs from three donors were purified by magnetic activated cell sorting (MACS)-assisted STRO-1-positive cell enrichment (+), colony derivation (c), or a combination of both (c/+). Immunophenotype, clonogenicity, stemness marker expression, senescence, and proliferation were analyzed. Multilineage differentiation was assessed by qPCR, immunohistochemistry, and extracellular matrix mineralization. To confirm the credibility of the results, repeated measures analysis and post hoc p-value adjustment were applied. All hDPSC fractions expressed STRO-1 and were similar for several surface markers, while their clonogenicity and expression of CD10/44/105/146, and 166 varied with the purification method. (+) cells proliferated significantly faster than (c/+), while (c) showed the highest increase in metabolic activity. Colony formation was most efficient in (+) cells, which also exhibited the lowest cellular senescence. All hDPSCs produced mineralized extracellular matrix. Regarding osteogenic induction, (c/+) revealed a significant increase in mRNA expression of COL5A1 and COL6A1, while osteogenic marker genes were detected at varying levels. (c/+) were the only population missing BDNF gene transcription increase during neurogenic induction. All hDPSCs were able to differentiate into chondrocytes. In summary, the three hDPSCs populations showed differences in phenotype, stemness, proliferation, and differentiation capacity. The data suggest that STRO-1-positive cell enrichment is the optimal choice for hDPSCs purification to maintain hDPSCs stemness. Furthermore, an (immuno) phenotypic characterization is the minimum requirement for quality control in hDPSCs studies.

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