Ultrasound-assisted dispersive liquid-liquid microextraction followed by gas chromatography–mass spectrometry for determination of parabens in human breast tumor and peripheral adipose tissue

Xiong, Shanbai; Liang, Jian; Zheng, Luxia; Wang, Hong; Wang, Zi; Ji, Qiuyi; Chen, Qiaoli; Lv, Qianzhou

doi:10.1016/j.jchromb.2018.08.004

Cited by 22 publications

(7 citation statements)

References 41 publications

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“…The following concentrations were used for PP, MP, and BP: 0.002 µM, 0.02 µM, 0.2 µM, 2 µM, and 20 µM. These concentrations fall within the range of what has been measured in biological samples in the NHANES biomonitoring study [14][15][16][17][18][19][20] and in breast tissue [21,22,[26][27][28][29]39]. Treatments were done in triplicates, and experiments were repeated six times.…”

Section: Cell Viabilitymentioning

confidence: 99%

“…Estrogenic activity has been detected in personal care products (e.g., oil hair lotion, intensive skin lotion) commonly used by Black women, thereby potentially increasing the risk for breast cancer in this under-resourced community [11]. Studies have shown that parabens are present in normal and in tumor breast tissue [26][27][28][29]. However, these studies did not include breast tissue from Black women.…”

Section: Introductionmentioning

confidence: 99%

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Parabens Promote Pro-Tumorigenic Effects in Luminal Breast Cancer Cell Lines with Diverse Genetic Ancestry

Tapia

McDonough

Cauble

et al. 2022

Preprint

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One in 8 women will develop breast cancer in their lifetime. Yet, the burden of disease is greater in Black women. Black women have a 40% higher mortality rate compared to White women, and a higher incidence of breast cancer at age 40 and younger. While the underlying cause of this disparity is multifactorial, exposure to endocrine disrupting chemicals (EDCs) in hair and other personal care products has been associated with an increased risk of breast cancer. Parabens are known EDCs that are commonly used as preservatives in hair and other personal care products, and Black women are disproportionately exposed to products containing EDCs. Studies have shown that parabens impact breast cancer cell proliferation, death, migration/invasion, and metabolism, as well as gene expression in vitro. However, these studies were conducted using cell lines of European ancestry; to date, no studies have utilized breast cancer cell lines of West African ancestry to examine the effects of parabens on breast cancer progression. Like breast cancer cell lines with European ancestry, we hypothesize that parabens promote pro-tumorigenic effects in breast cancer cell lines of West African ancestry. Luminal breast cancer cell lines with West African ancestry (HCC1500) and European ancestry (MCF-7) were treated with biologically relevant doses of methylparaben, propylparaben, and butylparaben. Following treatment, estrogen receptor target gene expression and cell viability were examined. We observed altered estrogen receptor target gene expression and cell viability that was paraben- and cell-line specific. This study provides greater insight into the tumorigenic role of parabens in the progression of breast cancer in Black women.

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Section: Cell Viabilitymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Parabens Promote Pro-Tumorigenic Effects in Luminal Breast Cancer Cell Lines with Diverse Genetic Ancestry

Tapia

McDonough

Cauble

et al. 2022

Preprint

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“…Parabens, esters of 4-hydroxybenzoic acid, which differ only in the ester group and may be an alkyl or benzyl group, are the most popular and widely used preservatives in food, pharmaceutical, and cosmetic products due to their broad spectrum of antimicrobial activity. , However, recent studies have shown that parabens possess estrogen agonist activity, being is even detected in human breast tumor tissue with an average concentration of 20 ng g –1 of tissue. − The widespread use of these compounds has led to environmental contamination . Parabens were detected in surface waters, soils and sediments, and indoor biota. , These compounds can be released into the aqueous environment mainly through wastewater treatment discharges and also as runoff from nonpoint sources and deposition of particles from the atmosphere .…”

Section: Introductionmentioning

confidence: 99%

Reactive Adsorption of Parabens on Synthesized Micro- and Mesoporous Silica from Coal Fly Ash: pH Effect on the Modification Process

et al. 2020

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Parabens are widely used as preservatives in food, pharmaceutical, and cosmetic products. These compounds are known for their estrogen agonist activity. This research investigates the synthesis of micro- and mesoporous silica from coal fly ash at different pH values (13, 11, 9, and 7) as well as its use as an adsorbent for the removal of parabens. The materials were characterized, and X-ray fluorescence (XRF) analysis revealed that the fly ash acid treatment reduced the presence of aluminum, iron, and calcium oxides and also that silica synthesized at lower pH values (7 and 9) showed a higher SiO2 content. X-ray diffraction (XRD) and scanning electron microscopy (SEM) analyses revealed microporous silica formation for silica synthesized at pH 13 and mesoporous silica at pH 7, 9, and 11. Adsorption tests were performed with materials, and FA-AT7 showed a higher adsorption capacity. The effect of factors (A) adsorbent mass, (B) initial paraben concentration, and (C) agitation rate on the adsorption process was studied for the FA-AT7 adsorbent using a factorial experimental design. Standardized Pareto charts revealed a negative effect of factor A, positive effect of factor B, and negative interaction effects of factors A–B for all studied parabens. Isotherms and multicomponent kinetic studies were performed. A linear type-III isotherm was obtained, and adsorption equilibrium was reached at approximately 10 min.

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“…Additionally, human placenta tissue samples have been pretreated using solid‐phase matrix dispersion with UHPLC–MS/MS, achieving a simple analysis procedure (Vela‐Soria et al, 2014). Moreover, an easy‐to‐use and environmentally friendly method permitted the extraction of parabens from human breast tumors and peripheral adipose tissue by ultrasound‐assisted dispersive liquid–liquid microextraction coupled with GC–MS (Shen et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

An improved fabric‐phase sorptive extraction protocol for the determination of seven parabens in human urine by HPLC–DAD

Rigkos

Alampanos

Kabir

et al. 2020

Biomedical Chromatography

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An improved fabric‐phase sorptive extraction (FPSE) protocol has been developed and validated herein for the simple, fast, sensitive and green determination of seven parabens—methyl paraben, ethyl paraben, propyl paraben, butyl paraben, isopropyl paraben, isobutyl paraben and benzyl paraben—in human urine samples by HPLC–DAD. The mobile phase consisted of ammonium acetate (0.05 m) and acetonitrile, while total analysis time was 13.2 min. Sol–gel poly (tetrahydrofuran) coated FPSE membrane resulted in optimum extraction sensitivity for the seven parabens. The novel FPSE medium as well as the improved and faster sample preparation procedure resulted in lower limit of detection and quantitation values in comparison with previously reported methods. The separation was carried out using an RP‐HPLC method with a Spherisorb C18 column and a flow rate of 1.4 ml/min. The validation of the analytical method was carried out by means of linearity, precision, accuracy, selectivity, sensitivity and robustness. For all seven parabens, the limits of detection and quantitation were 0.003 and 0.01 μg/ml, respectively. Relative recovery rates were between 86.3 and 104%, while RSD values were <12.6 and 19.3% for within‐ and between‐day repeatability, respectively. The method was subsequently applied to real human urine samples.

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Ultrasound-assisted dispersive liquid-liquid microextraction followed by gas chromatography–mass spectrometry for determination of parabens in human breast tumor and peripheral adipose tissue

Cited by 22 publications

References 41 publications

Parabens Promote Pro-Tumorigenic Effects in Luminal Breast Cancer Cell Lines with Diverse Genetic Ancestry

Parabens Promote Pro-Tumorigenic Effects in Luminal Breast Cancer Cell Lines with Diverse Genetic Ancestry

Reactive Adsorption of Parabens on Synthesized Micro- and Mesoporous Silica from Coal Fly Ash: pH Effect on the Modification Process

An improved fabric‐phase sorptive extraction protocol for the determination of seven parabens in human urine by HPLC–DAD

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