Ultrashort peptides induce biomineralization

Ding, Yanan; Cai, Meili; Niu, Pingping; Zhang, Han; Zhang, Shaoqing; Sun, Yao

doi:10.1016/j.compositesb.2022.110196

Cited by 12 publications

(11 citation statements)

References 45 publications

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“…Moreover, with significant disadvantages of limited supplies, discomfort at the harvest site, immunogenicity, and transmission of infectious diseases, there is a growing need for developing synthetic grafts for the treatment of damaged or diseased mineralized tissues. , Because of the hierarchical complexity of these mineralized tissues, fabrication of ideal synthetic grafts that can fulfill the requirement is still a challenge for material scientists. Mimicking the biological process to construct these mineralized structures has gained attention in the recent years. − A close consideration of the structural composition of bone has revealed that a complex combination of calcium phosphate in the form of the mineral hydroxyapatite, as well as collagenous fibrous proteins, makes up bone and teeth. Bone is a unique tissue with extraordinary mechanical and biological properties due to its intricate nanostructure. , It has been realized that the extracellular matrix, particularly connective tissues with their collagen fibers, is crucial for force transmission and maintenance of the bone structure.…”

Section: Introductionmentioning

confidence: 99%

Cooperative Calcium Phosphate Deposition on Collagen-Inspired Short Peptide Nanofibers for Application in Bone Tissue Engineering

Pal¹,

Roy²

2023

Biomacromolecules

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In recent years, immense attention has been devoted over the production of osteoinductive materials. To this direction, collagen has a dominant role in developing hard tissues and plays a crucial role in the biomineralization of these tissues. Here, we demonstrated for the first time the potential of the shortest molecular pentapeptide domain inspired from collagen toward mineralizing hydroxyapatite on peptide fibers to develop bone-filling material. Our simplistic approach adapted the easy and facile route of introducing the metal ions onto the peptide nanofibers, displaying adsorbed glutamate onto the surface. This negatively charged surface further induces the nucleation of the crystalline growth of hydroxyapatite. Interestingly, nucleation and growth of the hydroxyapatite crystals lead to the formation of a self-supporting hydrogel to construct a suitable interface for cellular interactions. Furthermore, microscopic and spectroscopic investigations revealed the crystalline growth of the hydroxyapatite onto peptide fibers. The physical properties were also influenced by this crystalline deposition, as evident from the hierarchical organization leading to hydrogels with enhanced mechanical stiffness and improved thermal stability of the scaffold. Furthermore, the mineralized peptide fibers were highly compatible with osteoblast cells and showed increased cellular biomarkers production, which further reinforced the potential application toward effectively fabricating the grafts for bone tissue engineering.

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Section: Introductionmentioning

confidence: 99%

Cooperative Calcium Phosphate Deposition on Collagen-Inspired Short Peptide Nanofibers for Application in Bone Tissue Engineering

Pal¹,

Roy²

2023

Biomacromolecules

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“…The peptide has a two-spaced arrangement of acidic amino acids ASP, which is beneficial to mineralization. What's more, through an analysis of the sequence of the dentin matrix protein 1 (DMP1), Ding et al 64 identified a notable abundance of SESSE amino acids, which also had a two-spaced arrangement of the acidic amino acid GLU. These observations strongly imply that peptides with specific sequence exert a positive influence on the mineralization processes.…”

Section: Inorganicmentioning

confidence: 99%

Nucleation Domains in Biomineralization: Biomolecular Sequence and Conformational Features

Huang,

Zhang,

Mao

et al. 2023

Inorg. Chem.

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Biomolecules play a vital role in the regulation of biomineralization. However, the characteristics of practical nucleation domains are still sketchy. Herein, the effects of the representative biomolecular sequence and conformations on calcium phosphate (Ca-P) nucleation and mineralization are investigated. The results of computer simulations and experiments prove that the line in the arrangement of dual acidic/essential amino acids with a single interval (Bc (Basic) -N (Neutral) -Bc-N-Ac (Acidic)-NN-Ac-N) is most conducive to the nucleation. 2αhelix conformation can best induce Ca-P ion cluster formation and nucleation. "Ac-× × × -Bc" sequences with α-helix are found to be the features of efficient nucleation domains, in which process, molecular recognition plays a non-negligible role. It further indicates that the sequence determines the potential of nucleation/mineralization of biomolecules, and conformation determines the ability of that during functional execution. The findings will guide the synthesis of biomimetic mineralized materials with improved performance for bone repair.

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“…Biomineralization is a common natural phenomenon involving organisms guiding and regulating the formation of inorganic minerals. 1,2 Within biomineralization, organic matrices, including organic molecules, polypeptides, proteins, and nucleic acids, act as nucleation sites for inorganic minerals, influencing crystal growth and assembly in biological environments, [3][4][5] and yielding ordered organic-inorganic composites with multi-stage structures. Several examples of these biomineral tissues include shells, teeth, bones, and biological shells.…”

Section: Introductionmentioning

confidence: 99%

Protein-guided biomimetic nanomaterials: a versatile theranostic nanoplatform for biomedical applications

Zhang,

Pan,

Chen

et al. 2024

Nanoscale

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Ultrashort peptides induce biomineralization

Cited by 12 publications

References 45 publications

Cooperative Calcium Phosphate Deposition on Collagen-Inspired Short Peptide Nanofibers for Application in Bone Tissue Engineering

Cooperative Calcium Phosphate Deposition on Collagen-Inspired Short Peptide Nanofibers for Application in Bone Tissue Engineering

Nucleation Domains in Biomineralization: Biomolecular Sequence and Conformational Features

Protein-guided biomimetic nanomaterials: a versatile theranostic nanoplatform for biomedical applications

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