Ultrasensitive electrochemical immuno-sensing platform based on gold nanoparticles triggering chlorpyrifos detection in fruits and vegetables

Talan, Anita; Mishra, Annu; Eremin, Sergei A.; Narang, Jagriti; Kumar, Ashok; Gandhi, Sonu

doi:10.1016/j.bios.2018.01.013

Cited by 128 publications

(36 citation statements)

References 38 publications

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“…The obtained pellet was re-suspended in deionized water (DW). The resuspended pellet was spiked with DNA (1 fM) and exposed to probe modified FTO electrode [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia. coli Shiga Toxin DNA

et al. 2018

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The present study was aimed to develop “fluorine doped” tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM–100 µM with a low detection limit of 1 fM where the current response increased linearly with Escherichia coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity.

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“…The obtained pellet was re-suspended in deionized water (DW). The resuspended pellet was spiked with DNA (1 fM) and exposed to probe modified FTO electrode [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia. coli Shiga Toxin DNA

et al. 2018

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“…The immunochemical methods that apply affinity-based detection strategies employ antibody-based platforms for AFB1 detection. Antibody-based format has several drawbacks that include tedious antibody generation process followed by skilled labor required for its mass production (Chen et al, 2014 ; Gandhi et al, 2018a , b ). Nucleic acid-based approaches pave way for novel strategy that embodies a stable and economical alternative to the antibodies (Wandtke et al, 2015 ).…”

Section: Discussionmentioning

confidence: 99%

Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its Evaluation Onto Various Field Samples

et al. 2018

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The present study was aimed to develop a novel antibody-aptamer based hybrid detection strategy for specific and sensitive detection of aflatoxin B1 (AFB1) from contaminated food grains. The study comprises generation of ssDNA aptamers and anti-AFB1 IgG against AFB1 toxin. The generated bio-probes (aptamers and antibodies) were further characterized for their specificity and sensitivity using indirect ELISA. The generated aptamers namely AFB1a and AFB1b showed prominent reactivity and selectivity against AFB1 toxin. These aptamers were further characterized for their secondary structures and dG values were determined as −4.6 and −2.75 Kcal/mol, respectively. The detection limit (LOD) of AFB1a and anti-AFB1 IgG was determined as 5 and 10 ng/mL, respectively. The characterized aptamers and antibodies against AFB1 were used to develop the sandwich immunoassay. Anti AFB1 IgG was used as a capturing antibody whereas anti-AFB1a aptamer was used as its revealing partner in the assay. The limit of detection (LOD) of the immunoassay was determined to be 5 ng/mL of AFB1 standard toxin and showed no cross-reactivity with closely related mycotoxins. To assess the reliability of the developed method, several field samples contaminated with aflatoxin B1 was included in the study and results were validated with commercial AFB1-ELISA Kit. Additionally, the spiking studies were also carried out to demonstrate the consistency and dependability of the developed hybrid sandwich immunoassay wherein the toxins recovered were found to be ranging between 73 and 98.80% with the LOD at 5 ng/mL. In conclusion, the developed method may find the better utility in routine food testing laboratories for assessment of AFB1.

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“…Gold nanoparticles (19 nm) were prepared by citrate reduction method. [32][33][34][35] Aggregation of gold nanoparticles is due to strong van der Waals forces at high salt concentrations that alter the charges on the surface of AuNPs resulting in aggregation. 36 Scheme 1 depicted colorimetric method for A B1 aptasensing.…”

Section: Aggregation Assaymentioning

confidence: 99%

Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

et al. 2020

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Ultrasensitive electrochemical immuno-sensing platform based on gold nanoparticles triggering chlorpyrifos detection in fruits and vegetables

Cited by 128 publications

References 38 publications

Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia. coli Shiga Toxin DNA

Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia. coli Shiga Toxin DNA

Development of Hybrid IgG-Aptamer Sandwich Immunoassay Platform for Aflatoxin B1 Detection and Its Evaluation Onto Various Field Samples

Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

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