Microfluidic paper device for rapid detection of aflatoxin B1 using an aptamer based colorimetric assay

Abstract

Contamination of milk by mycotoxins is a serious problem worldwide.

Graphene based sensors

“…Biosensors are classified based on the different type of recognition element, transducer or amplifier used. On the basis of the type of recognition element [12] , biosensors are often classified into enzymatic sensors [14] , [15] , [16] , immunosensors [13] , [17] , [18] , [19] , DNA based sensors [20] , [21] , [22] , aptamer based sensors [23] , [24] or whole cell microbial biosensors [25] , [26] , [27] , [28] . Based on the type of transducer, biosensors can be categorized into four types- Electrochemical biosensors [29] , [30] , Optical biosensors [31] , [32] , [33] , Piezoelectric biosensors [34] , [35] , [36] , and Thermal biosensors [37] , [38] , [39] .…”

Advances in Colorimetric Strategies for Mycotoxins Detection: Toward Rapid Industrial Monitoring

“…On the other hand, quantification of the results can be made with a smartphone. As reported in Table 4 , the proposed µPAD by Kasoju et al [ 88 ] for AFB1 detection showed high sensitivity, low-cost, short detection time (>1 min), and portability. Moreover, the proposed µPAD for DON detection has a great potential for industrialization [ 90 ].…”

“…Although there are a number of well-developed systems for immunoassay in microfluidic (lab-on-chip) format, the use of aptamers in similar devices is on very beginning. Kasoju et al developed a microfluidic paper-based analytical device (µPAD) for AFB1 detection using aptamer as recognition element [ 88 ]. The hydrophobic barriers were developed on the Whatman filter paper using photolithography.…”

“… ( a ) Schematic representation of the microfluidic immunoassay with smartphone data acquisition for multiplexed mycotoxin detection; ( b ) microfluidic immunoassay for AME detection: ( 1 ) The 3D structural diagram of the fabricated microfluidic chip, ( 2 ) The schematic of the AME detection using the amplified microfluidic immunoassay, and ( 3 ) The colorimetric detection by UV spectroscopy and smartphone imaging APP; ( c ) Development of a µPAD using aptamer and for AFB1 detection based on salt-induced aggregation of AuNPs in the presence of analyte. Reproduced from [ 83 , 87 , 88 ], respectively, with permission. …”

eCovSens-Ultrasensitive Novel In-House Built Printed Circuit Board Based Electrochemical Device for Rapid Detection of nCovid-19 antigen, a spike protein domain 1 of SARS-CoV-2

“…Biosensors have the advantage of being sensitive, specific, stable, easy to use, require less sample size, time, portable, and most importantly can be customised to detect the target analyte of interest. Immunosensors can be used to detect toxins (Kasoju et al, 2020a), narcotic drugs Singh et al, 2017;Tey et al, 2010) viruses (Kerry et al, 2019) by use of different bioreceptors such as deoxyribonucleic acid (DNA) (Jiang et al, 2005;Labuda et al, 2009), enzymes (Ilangovan et al, 2006;Jawaheer et al, 2003), peptides (Gandhi et al, 2016), aptamers (Kasoju et al, 2020b), antibody (Islam et al, 2019;Roberts et al, 2019). Electrochemical biosensors are considered as a reliable tool for infectious disease detection as they remain unaffected by sample absorbance or turbidity (Bakker, 2004).…”