Development of MoSe2 Nano-Urchins as a Sensing Platform for a Selective Bio-Capturing of Escherichia coli Shiga Toxin DNA

Abstract

The present study was aimed to develop “fluorine doped” tin oxide glass electrode with a MoSe2 nano-urchin based electrochemical biosensor for detection of Escherichia coli Shiga toxin DNA. The study comprises two conductive electrodes, and the working electrodes were drop deposited using MoSe2 nano-urchin, and DNA sequences specific to Shiga toxin Escherichia coli. Morphological characterizations were performed using Fourier transforms infrared spectrophotometer; X-ray diffraction technique and scanning electron microscopy. All measurements were done using methylene blue as an electrochemical indicator. The proposed electrochemical geno-sensor showed good linear detection range of 1 fM–100 µM with a low detection limit of 1 fM where the current response increased linearly with Escherichia coli Shiga toxin dsDNA concentration with R2 = 0.99. Additionally, the real sample was spiked with the dsDNA that shows insignificant interference. The results revealed that the developed sensing platform significantly improved the sensitivity and can provide a promising platform for effective detection of biomolecules using minute samples due to its stability and sensitivity.

Sensing beyond Senses: An Overview of Outstanding Strides in Architecting Nanopolymer-Enabled Sensors for Biomedical Applications

“…The signal, generated by the varying rate of hydrogen peroxide production due to bacterial probing in the macrophage cells of mice as a function of concentration and time, was found to be different for all of the three varieties of gram-negative bacteria. These results indicate the dynamical and adaptive nature of the immune system after an infection and a similar applicability on real samples is noticed in a specific detection and morphological characterization of a toxin DNA of Escherichia coli through MoSe 2 nano-urchin’s fabrication, and methylene blue as hybridization indicator, showing differential interaction [ 35 ] with repeatability and accuracy. The accuracy observed matches with the sensing platform composed of polymeric nanocapsules used in quantitatively assessing the interplay of RBCs [ 36 ] without affecting the energy of the interaction process with the aid of optical tweezers.…”

An Electroanalytical Flexible Biosensor Based on Reduced Graphene Oxide-DNA Hybrids for the Early Detection of Human Papillomavirus-16

“…Figure 3 b indicates the effect of pH on the sensor interface kinetics. The alkaline environment is favourable for MB whereby the latter is reported to form cations at higher pH, in which case OH − is adsorbed to the PDNA-modified bio-electrode surface to form negatively charged adsorption centres, which promotes the adsorption of MB ions [ 35 , 36 ]. This is indicated by high I pa at pH 7.4 and its drastic reduction, followed by shifting of the voltammograms towards higher potentials, at lower pH which indicates slow electron transfer at the latter.…”

Paper-based analytical devices for virus detection: Recent strategies for current and future pandemics

“…Methylene blue (MB), which is a cationic dye, is used for electrochemical detection of target DNA due to the interaction between free guanine base and the cationic amino groups of MB. As a result of hybridization between ssDNA and the target DNA, the signal is decreased due to the reduced availability of free guanine [ 167 ]. Although nucleic acid-based ePADs show high specificity and stability, efficient hybridization is a challenging task to obtain desired sensitivity [ 168 , 169 ].…”