Ultrasensitive determination of DNA oxidation products by gas chromatography–tandem mass spectrometry and the role of antioxidants in the prevention of oxidative damage

Dawbaa, Sam; Aybastıer, Önder; Demir, Cevdet

doi:10.1016/j.jchromb.2017.03.014

Cited by 13 publications

(5 citation statements)

References 38 publications

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“…Regarding grape tendrils, the data on the phenolic contents and the biological actions are very few or missing (derived from caffeic acids and antioxidant activity). Only Dawbaa et al (2017) and Fraternale et al (2011) obtained lower amounts of TPC and TFC [37,38].…”

Section: Total Phenolic Contents and Antioxidant Activitymentioning

confidence: 97%

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

et al. 2020

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Winery industry by-products have a great reuse potential in the pharmaceutical and cosmetic fields due to their bioactive compounds. This study investigates the phytochemical profile and the bioactivity of Vitis vinifera variety Fetească neagră tendrils extract (TE) and leaves extract (LE), intended to be used in oral hygiene products recommended in periodontal disease. The evaluation of the phenolic content was performed by colorimetric analysis. Liquid chromatography coupled with mass spectrometry was used to determine the chemical profile of the two extracts obtained from V. vinifera. Moreover, the antioxidant activity of the extracts was determined by spectrophotometric methods, as well as on human gingival fibroblasts (HGF) cell line. The cytocompatibility and cytoprotective effect against nicotine-induced cytotoxicity were tested, as well as the anti-inflammatory and antimicrobial effects. The TE showed higher total polyphenolic content, rich in rutin, compared to the leaves extract that displayed important amounts of isoquercitrin. The antioxidant effect was confirmed by both non-cellular and cellular tests. The cytocompatibility of the extracts was confirmed at a wide range of concentrations. The cytoprotective effect was demonstrated in HGF exposed to cytotoxic doses of nicotine; 300 µg/mL of both tested extracts decreased nicotine toxicity by approximately 20%. When challenged with E. coli polysaccharides, in HGF cells co-exposed to TE and LE, a reduction in the release of proinflammatory cytokines (IL-8, IL-6 and IL-1β) was observed. The extracts were both able to reduce the levels of reactive oxygen species and inflammatory cytokines, and had notable antimicrobial effects on pathogenic bacteria associated with periodontitis.

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Section: Total Phenolic Contents and Antioxidant Activitymentioning

confidence: 97%

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

et al. 2020

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“…It is known that many diseases such as neurodegenerative, cardiovascular, cancer diseases and ageing problems are related to the oxidation of DNA (Dawbaa et al, 2017). So the detection of DNA damage has got great importance.…”

Section: Introductionmentioning

confidence: 99%

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

Karkar¹,

Şahin²,

Güneş³

2018

GIDA

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Chestnut bee pollen is a high antioxidative natural bee product. In this study the phenolic compounds in chestnut bee pollen were determined by HPLC-DAD system. Ethanol, water and methanol were used for the extraction of antioxidant compounds from chestnut bee pollen. The total phenolic contents and antioxidant capacities of extracts were determined by Folin-Ciocalteu, CHROMAC (Cr(VI) reduction antioxidant capacity) and FRAP (ferric reducing antioxidant power) methods, respectively. When compared the amounts of phenolic compounds and spectroscopic results (total phenolic contents and antioxidant capacities), the most suitable solvent was found ethanol for the extraction of antioxidant compounds from bee pollen. The major phenolic compounds in the chestnut bee pollen were determined as pinocembrin, chrysin, galangin and hyperoside with the contents of 1.246, 0.332, 0.122 and 0.516 mg/g of bee pollen, respectively. And also it was determined that the chestnut bee pollen was inhibited 11% of DNA oxidation in Fenton medium.

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“…Lyophilized calf thymus DNA (5 mg in 10 ml) was dissolved in distilled water; then, the DNA stock solution was incubated at 4°C overnight. After dilution of the DNA solution (1:10), it was incubated at 4°C for one night, and the concentration of DNA was calculated as µg/ml, as described in the literature (Dawbaa, Aybastier, & Demir, ).…”

Section: Methodsmentioning

confidence: 99%

“…Before GC‐MS/MS analysis, the samples were derivatized according to the literature (Dawbaa et al, ). Trace 1,300 GC and TSQ 8,000 Evo from Thermo Scientific (USA) with an electron impact ionization mode system were used for determining products from the oxidative DNA damage.…”

Section: Methodsmentioning

confidence: 99%

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The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

Şahin

Karkar

2019

J Food Biochem

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Chestnut bee pollen has potential nutritional and medicinal effects and is an important natural bee product. This study focused on the investigation of the antioxidant capacity and DNA damage inhibition ability of chestnut bee pollen (CBP) from Bursa (Turkey). The phenolic compounds (rosmarinic acid, vitexin, hyperoside, pinocembrin, trans‐chalcone, apigenin, protocatechuic, and galangin) and carotenoids in CBPE were determined by HPLC‐DAD (high‐performance liquid chromatography‐diode array detection). Additionally, the protective ability of CBPE against DNA damage by oxidation was investigated. In this study, it was determined that CBPE has a high total phenolic compound content, and the antioxidant capacity of CBPE inhibits DNA oxidation (34% reduction of DNA damage in Fenton reaction media). This study could reveal new information regarding the use of CBPE as a protective agent for DNA in the future. Practical applications Phenolic compounds and carotenoids prevent some diseases because of their important biological activities. One of the potential food sources chestnut bee pollen contains sugar, carbohydrates, amino acids, proteins, lipids, vitamins, hormones, enzymes, and flavonoids. Chestnut bee pollen, which has protective activity against DNA oxidation, could be an excellent potential source of a protective agent against some degenerative diseases through future applications.

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Ultrasensitive determination of DNA oxidation products by gas chromatography–tandem mass spectrometry and the role of antioxidants in the prevention of oxidative damage

Cited by 13 publications

References 38 publications

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

Phytochemical Profile and Biological Activities of Tendrils and Leaves Extracts from a Variety of Vitis vinifera L.

Antioxidative Effect of Turkish Chestnut Bee Pollen on Dna Oxidation System and Its Phenolic Compounds

The antioxidant properties of the chestnut bee pollen extract and its preventive action against oxidatively induced damage in DNA bases

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