Ultrasensitive Detection of Ribonucleic Acid Biomarkers Using Portable Sensing Platforms Based on Organic Electrochemical Transistors

Abstract

The analysis of ribonucleic acid (RNA) plays an important role in the early diagnosis of diseases and will greatly benefit patients with a higher cure rate. However, the low abundance of RNA in physiological environments requires ultrahigh sensitivity of a detection technology. Here, we construct a portable and smart-phone-controlled biosensing platform based on disposable organic electrochemical transistors for ultrasensitive analysis of microRNA (miRNA) biomarkers within 1 h. Due to their inherent amplification function, the devices can detect miRNA cancer biomarkers from little-volume solutions with concentrations down to 10–14 M. The devices can distinguish blood miRNA expression levels at different cancer stages using a 4T1 mouse tumor model. The technique for ultrasensitive and fast detection of RNA biomarkers with high selectivity opens a window for mobile diagnosis of various diseases with low cost.

Rapid diagnosis of COVID-19vianano-biosensor-implemented biomedical utilization: a systematic review

“…Several types of OECT-based nano-biosensors are effectively capable of spotting the protein present in corona virus spikes. 38,39…”

“…Several types of OECT-based nano-biosensors are effectively capable of spotting the protein present in corona virus spikes. 38,39 Zamzami's group developed an electrochemical nanobiosensor composed of carbon nanotube FET (CNT-FET) for detecting the SARS-CoV-2 spike protein (S1) in a patient's saliva. 40 The detection is rapid (2-3 min), accurate, cost-effective, simple, and quantitative.…”

Organic photoelectrochemical transistor detection of tear lysozyme

“…13 Our group developed an integrated PEC nanotool for single-cell drug delivery and assessment of the corresponding treatment effect. 14 Organic electrochemical transistors (OECTs), [19][20][21][22][23][24] involving electrochemical doping/de-doping, represent another rapidly developing detection technology due to their attractive properties, e.g., miniaturized structure, intrinsic signal amplification, and compatibility for aqueous operation, which are necessary for biodetection. A typical OECT consists of a thin organic semiconductor, e.g., conducting polymer poly (3,4ethylenedioxythiophene):poly(styrene sulfonate) (PEDOT:PSS), coated on the channel (C) between the drain (D) electrode and the source (S) electrode, and a gate (G) electrode immersed with an electrolyte (E).…”

Design of a Portable Microfluidic Platform for EGOT-Based in Liquid Biosensing

“…In this way, it is possible to associate the shift of the output signal with the amount of analyte present in the electrolytic solution, allowing for a quantification of the tested biomolecule. EGOTs have already been proven to allow detection of low concentrations of different biomolecules (i.e., proteins [20,23,24], drugs [25,26], or ribonucleic acids [27,28]), achieving limits of detection that can permit the use of these device in real applications (i.e., lab-on-chip) [18].…”