Design of a Portable Microfluidic Platform for EGOT-Based in Liquid Biosensing

Segantini, Matteo; Parmeggiani, Matteo; Ballesio, Alberto; Palmara, Gianluca; Frascella, Francesca; Cocuzza, Matteo

doi:10.3390/s22030969

Cited by 8 publications

(5 citation statements)

References 46 publications

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“…Briefly, each mold slice was obtained by extruding VeroWhite ™ (Stratasys, United States, Minneapolis) photopolymer stacked layers and exposing the same while printing to UV radiation to induce crosslinking. The molds were placed in oven at 110 °C overnight, then a rinse in acetone was performed to remove the remaining photo-initiators from the structures and promote recombination reactions between the photo-initiator and residual high molecular weight species in the photopolymer, more details about the procedure can be found in a previous work (Segantini et al, 2022).…”

Section: Fabrication Of the Alveolus-on-a-chipmentioning

confidence: 99%

A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier

Licciardello,

Traldi,

Cicolini

et al. 2024

Front. Bioeng. Biotechnol.

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Several diseases affect the alveoli, and the efficacy of medical treatments and pharmaceutical therapies is hampered by the lack of pre-clinical models able to recreate in vitro the diseases. Microfluidic devices, mimicking the key structural and compositional features of the alveoli, offer several advantages to medium and high-throughput analysis of new candidate therapies. Here, we developed an alveolus-on-a-chip recapitulating the microanatomy of the physiological tissue by including the epithelium, the fibrous interstitial layer and the capillary endothelium. A PDMS device was obtained assembling a top layer and a bottom layer obtained by replica molding. A polycaprolactone/gelatin (PCL-Gel) electrospun membrane was included within the two layers supporting the seeding of 3 cell phenotypes. Epithelial cells were grown on a fibroblast-laden collagen hydrogel located on the top side of the PCL-Gel mats while endothelial cells were seeded on the basolateral side of the membrane. The innovative design of the microfluidic device allows to replicate both cell-cell and cell-extracellular matrix interactions according to the in vivo cell arrangement along with the establishment of physiologically relevant air-liquid interface conditions. Indeed, high cell viability was confirmed for up to 10 days and the formation of a tight endothelial and epithelial barrier was assessed by immunofluorescence assays.

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Section: Fabrication Of the Alveolus-on-a-chipmentioning

confidence: 99%

A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier

Licciardello,

Traldi,

Cicolini

et al. 2024

Front. Bioeng. Biotechnol.

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“…Physical modelling has emerged from several points of view as a fundamental aspect in the evolution of integrated bioelectronic devices. The design of microfluidic cartridges aimed at avoiding device contaminations [ 13 , 90 ], the description of physical mechanisms involved in EGOT operations [ 59 , 72 ], the proper co-design of an electronic device and functionalization strategy [ 55 ] and the correct interpretation of a biosensor’s response at different analytes [ 53 , 56 ]; these are all fundamental parts of the device engineering benefiting from the development of more accurate models. In the following section, we will focus on the last three aspects, skipping the design of microfluidic devices.…”

Section: Transistor-based Sensorsmentioning

confidence: 99%

“…Researchers from the University of Cagliari are instead developing new biosensors and devices for cellular analysis [ 11 ]. At the Polytechnics of Milano and Torino, researchers have been involved in the development of advanced technologies for new chip design and functionality [ 12 ] and for integration and sensing in microfluidic systems [ 13 ].…”

Section: Introductionmentioning

confidence: 99%

Organic Bioelectronics Development in Italy: A Review

et al. 2023

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In recent years, studies concerning Organic Bioelectronics have had a constant growth due to the interest in disciplines such as medicine, biology and food safety in connecting the digital world with the biological one. Specific interests can be found in organic neuromorphic devices and organic transistor sensors, which are rapidly growing due to their low cost, high sensitivity and biocompatibility. This trend is evident in the literature produced in Italy, which is full of breakthrough papers concerning organic transistors-based sensors and organic neuromorphic devices. Therefore, this review focuses on analyzing the Italian production in this field, its trend and possible future evolutions.

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“…A microfluidic platform hosting the chip and the gate electrode was specifically designed to conduct this study, inspired by our previously proposed concept of a portable sensing card. [24] Output and transfer characteristics, and cyclic voltammetry (CV) measurements have been performed on the two polymers in order to provide a complete characterization of the system under analysis.…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Investigation and Modeling of the Electrical Bias Stress in Electrolyte‐Gated Organic Transistors

Segantini

Ballesio

Palmara

et al. 2022

Adv Elect Materials

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is coupled with the gate electrode via an electrolyte. The application of a bias to a polarizable gate electrode may either induce the formation of two electrical double layers or allow the intercalations of ions in the OSC film. In the first case, the transistors are operating in the field-effect mode and they are called electrolyte-gated organic field-effect transistors (EGOFETs), while in the second case, an electrochemical doping occurs and the devices are named organic electrochemical transistors (OECTs). EGOTs can operate with voltages below 1 V and are extremely sensitive to any change that occurs either on the gate electrode or on the OSC. For this reason, these devices are exploited in biosensing applications for the detection of specific binding events occurring at the gate surface, which is functionalized to match the targeted analyte. Indeed, EGOTs can be used as biosensors to detect low concentrations of relevant biomolecules (proteins, [1][2][3] nucleic acids, [4,5] drugs [6][7][8] ), but the manufacturing of these devices for scale production and commercial applications is still far to be achieved. In fact, one of the main disadvantages of EGOTs is their poor operational stability, whose origins are still under investigation. [9][10][11] Several works in the literature concerning organic transistors address the instability related to the electrical bias stress (EBS), but most of the proposed models deal with solid-state organic field-effect transistor (OFET). [12,13] For this kind of devices, the EBS is responsible for mobile charges trapping either in the OSC material, or in the insulating dielectric, or at the OSC/dielectric interface. This mechanism is usually related to high-voltageinduced water electrolysis phenomena affecting adsorbed adventitious water molecules present on the oxide surface. [12,14] The EBS in EGOTs should be ascribed to different causes since the low operating voltages prevent any water electrolysis to occur. In addition to this, the different nature of the dielectric material avoids any charge accumulation in the dielectric, being the ions in the electrolyte free to move. Concerning the operational stability of EGOTs, only a limited number of works are found in the literature addressing the issue. For instance, Blasi et al. investigated the long-term stability of EGOFETs based on poly(3hexylthiophene) (P3HT), showing that the maximum current drift over time could be modeled with a biexponential function, in which two processes with different timescales occur. [15] Electrolyte-gated organic transistors (EGOTs) are emerging as an important tool in advanced biosensing applications. However, their widespread exploitation is still limited by their poor operational stability. In order to understand the causes of this unreliability, the proposed study focuses on the influence of electrical bias stress (EBS) on EGOTs operating in aqueous electrolytes. Poly(3-hexylthiophene) (P3HT)-and poly[3-(5-carboxypentyl)thiophene)] (P3CPT)-based transistors are studied under the application o...

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Design of a Portable Microfluidic Platform for EGOT-Based in Liquid Biosensing

Cited by 8 publications

References 46 publications

A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier

A miniaturized multicellular platform to mimic the 3D structure of the alveolar-capillary barrier

Organic Bioelectronics Development in Italy: A Review

Investigation and Modeling of the Electrical Bias Stress in Electrolyte‐Gated Organic Transistors

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