Ultrasensitive aptasensor for isolation and detection of circulating tumor cells based on CeO2@Ir nanorods and DNA walker

Programmable Analytical Feature of Ratiometric Electrochemical Biosensor by Alternating the Binding Site of Ferrocene to DNA Duplex for the Detection of Aflatoxin B1†

“…Signal amplification strategy has been frequently employed to enhance the sensitivity of single signal‐based biosensor, e.g ., hybridization chain reaction (HCR), and DNA walker. [ 25‐26 ] Meanwhile, many reports have been focused on the modulation of dynamic range of biosensor by introducing probes with different affinities for the same target or photocleavable capture probe to tune the affinity for the target. [ 27‐28 ] All these strategies could efficiently tune the analytical feature of single signal‐based biosensors; for ratiometric biosensor, the situation may be different.…”

Nanostructured Ceria: Biomolecular Templates and (Bio)applications

“…DNA walker strands display RNAse-like activity and cleave the RNA of chimeric DNA/RNA oligonucleotides (D-RNA). The name comes from the fact that the strand displacement reaction causes the DNA walker strands to “walk forward” along the adjacent D-RNA, thus continuously cleaving it and amplifying the signal through the release of many probes [ 179 ].…”

Electrochemical Sensing Directions for Next-Generation Healthcare: Trends, Challenges, and Frontiers

“…For that purpose, redox cycling and more recently novel and powerful approaches based on custom designed single stranded DNA probes have been proposed. Some of the latter include DNA walker technologies, , rolling circle amplification (RCA), , strand displacement or toehold-mediated DNA amplification . These strands have a dual role in these nanoarchitectures: (i) recognition of the target analyte and (ii) catalyze themselves (DNAzyme) or participate in redox reactions and serve as an anchoring point for enzymes or redox probes , responsible for the electrochemical signals.…”