Ultramicroscopic Changes of Rats Parathyroid Glands and Thymus After Single Administration Of Cyclophosphamide at the Different Periods Of Observation

Abstract

Introduction: Cyclophosphamide has wide spectrum usage as first-line drug in cancer chemotherapy that is why a detailed study of its effect on individual cell populations is of great interest for science and practice. The interaction of the nervous, immune and endocrine systems plays essential role in the homeostasis maintaining. The aim: This study aimed to investigate the ultramicroscopic changes that occur in the parathyroid glands and thymus of male rats after cyclophosphamide administration. Materials and methods: Twenty-four WAG matured male rats were divided randomly into two groups. The first group served as control and was provided 0.9% soluble sodium chloride. The second group received cyclophosphamide in a dosage 200 mg/kg of body weight of animal by intramuscular single injection. All animals were sacrificed on the 7th and 30th day after injection. Parathyroid gland and thymus specimens were dissected out and processed for electron microscopy. Results: The results showed that cyclophosphamide exposure caused marked ultramicroscopic changes in rats parathyroid glands and thymus. On the 7th day after immunosuppression, the nuclei of parathyrocytes have deep wavy invaginations, amount of the organelles that participate in the protein synthesis is reduced to a minimum in the cytoplasm of the chief cells. Characteristic feature is the appearance of numerous plasma cells and active macrophages in thymus. There is a tendency to normalization of the parathyroid structure on the 30th day after administration of cyclophosphamide and reduction of mitotic activity of lymphocytes in thymus, which points to the development of involution process. Conclusions: This data can be successfully extrapolated from experimental animals to humans.

Histological study of the role of CD34+ stem cells and mast cells in cyclophosphamide-induced thymic injury in rats and the possible attenuating role of melatonin

“…ERCs also showed significant ultrastructural changes. Similar findings with CP treatment have been observed by other authors (Arudchelvan et al 2005 ; Erokhina and Avilova 2019 ). These changes could be attributed to the cytotoxic effects of CP.…”

Morphometric Analysis of the Thymic Epithelial Cell (TEC) Network Using Integrated and Orthogonal Digital Pathology Approaches

“…The cellular component of the cTEC meshwork, also known as "cytoreticulum", is identified in both OTO-SEM and TEM by the characteristic presence of cytoplasmic tonofilaments, vacuoles, and the occasional presence of desmosomes between interconnecting cTEC projections (Fig. S4), as reported [68][69][70][71][72] . These distinguishing epithelial features provide validation of cTEC identity, especially when they are projected in proximity to cTEC nuclei, or when the cytoplasmic projection is shown to bud off the cTEC on the same z-plane (Fig.…”

Ultrastructural study of the thymus of Mugil liza Valenciennes, 1836 juveniles

“…The fine structural characteristics of the normal thymus have been described in many animal species [1] , [2] , [3] . Additionally, the thymus in humans has been described in detail both in normal and pathological conditions [4] , [5] , [6] .…”