Histological study of the role of CD34+ stem cells and mast cells in cyclophosphamide-induced thymic injury in rats and the possible attenuating role of melatonin

Shrief, Amira I; Hamed, Walaa H E; Mazroa, Shireen A.; Moustafa, Amal M.

doi:10.1007/s00418-023-02185-6

Cited by 3 publications

(2 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…APH and CP damage the histological structure changes of the rat spleen and thymus, including induction of disorganization in splenic structures, thymic apoptosis, hypocellularity and atrophy ( 35 , 36 ). H&E staining showed that the splenic cord, splenic sinus and trabecula in the red pulp (RP) were displayed clearly in the control group.…”

Section: Resultsmentioning

confidence: 99%

Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency rats

Su,

Liang,

Pan

et al. 2024

Exp Ther Med

View full text Add to dashboard Cite

Notoginseng saponins (NS) are the active ingredients in Panax notoginseng (Burk.) F.H. Chen (PN). NS can be transformed depending on how the extract is processed. Fermentation has been shown to produce secondary ginsenosides with increased bioavailability. However, the therapeutic effect of fermented NS (FNS) requires further study. The present study compared the compositions and activities of FNS and NS in blood deficiency rats, which resembles the symptoms of anemia in modern medicine, induced by acetylphenylhydrazine and cyclophosphamide. A total of 32 rats were randomly divided into control, model, FNS and NS groups. A blood deficiency model was established and then treatment was orally administered for 21 days. The results of component analysis indicated that some saponins transformed during the fermentation process resulting in a decrease of notoginsenoside R1, and ginsenosides Rg1, Rb1 and Re, and an increase in ginsenosides Rd, Rh2, compound K, protopanaxadiol and protopanaxatriol. The animal results showed that both FNS and NS increased the number of white blood cells (WBCs), red blood cells, hemoglobin, platelets and reticulocytes, and the levels of granulocyte-macrophage colony-stimulating factor (GM-CSF), erythropoietin (EPO) and thrombopoietin (TPO), decreased the G 0 /G 1 phase and increased G 2 /M phase, and decreased the apoptosis rate of bone marrow (BM) cells, which suggested a contribution to the recovery of hematopoietic function of the BM cells. FNS and NS increased the protein expression levels of the cytokines IL-4, IL-10, IL-12, IL-13, TGF-β, IL-6, IFN-γ and TNF-α, and the mRNA expression levels of transcription factors GATA binding protein 3 and T-box expressed in T cell (T-bet). FNS and NS treatment also increased the number of CD4 + T cells, and decreased the enlargement of the rat spleen and thymus atrophy, which indicated a protective effect on the organs of the immune system. The results of the present study demonstrated that compared with NS, FNS showed an improved ability to increase the levels of WBCs, lymphocytes, GM-CSF, EPO, TPO, aspartate aminotransferase, IL-10, IL-12, IL-13 and TNF-α, and the mRNA expression levels of T-bet, and decrease alanine aminotransferase levels. The differences seen for FNS treatment could arise from their improved bioavailability compared with NS, due to the larger proportion of hydrophobic ginsenosides produced during fermentation.

show abstract

Section: Resultsmentioning

confidence: 99%

Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency rats

Su,

Liang,

Pan

et al. 2024

Exp Ther Med

View full text Add to dashboard Cite

show abstract

“…CP probably inhibits cancerous cell proliferation. Studies have also shown that it can cause apoptosis in cancer cells in rat fetuses, lung cells, and, rat thymus (3). Ephedra pachyclada is a plant of the Ephedraceae family.…”

Section: Introductionmentioning

confidence: 99%

The protective effect of hydroalcoholic extract of Ephedra pachyclada leaves on ovarian damage induced by cyclophosphamide in rat: An experimental study

Abedi,

Nemati,

Ebrahimi

et al. 2023

IJRM

View full text Add to dashboard Cite

Background: Cyclophosphamide (CP) is an anticancer drug that acts as an alkylation agent after metabolism in the liver. CP has toxic effects on the body’s cells, especially the reproductive system’s function, and causes infertility. Moreover, medicinal plants have few side effects and are psychologically acceptable to patients. Objective: This study aimed to investigate the impact of Ephedra pachyclada hydroalcoholic extract (EPHE) on ovarian tissue and hypothalamic-pituitary-gonadal axis in rats treated with CP. Materials and Methods: In this experimental study, 48 adult female Wistar rats (180-200 gr, 9-10 wk) were randomly assigned to 6 experimental groups (n = 8/each): (a) control; (b) sham; (c) CP; (d) CP+250 mg/kg EPHE; (e) CP+500 mg/kg EPHE; (f) CP+1000 mg/kg EPHE. On the 29th day of the experiment, serum was collected; serum concentration of the luteinizing hormone, follicle-stimulating hormone, estrogen, progesterone, and antioxidant activity were measured. The number of ovarian follicles were also counted. Results: In the CP groups, serum concentrations of follicle-stimulating hormone and luteinizing hormone significantly increased, and estrogen and progesterone significantly decreased (p ≤ 0.05). EPHE significantly compensated for the complications caused by CP and 1000 mg/kg had the greatest effect. Antioxidant reduction by CP was significantly enhanced by EPHE, especially at higher doses (p ≤ 0.05). The number of primordial, primary, secondary, and Graafian follicles showed a significant decrease in CP groups and EPHE groups showed a significant increase compared to the CP. EPHE showed that the concentration of 1000 mg/kg was more effective than other doses (p ≤ 0.05). Conclusion: In addition to proving the effect of EPHE on the hypothalamic-pituitary-gonadal axis, our investigation showed antioxidant properties, which can be an effective factor in CP-treated rats. Key words: Ephedra, Cyclophosphamide, Ovary, Follicles, Hydroalcoholic extract.

show abstract

Anti-allergic drug screening in single living mast cells by means of a novel surface plasmon resonance imaging system

Wang,

Zhang,

et al. 2024

Sensors and Actuators B: Chemical

View full text Add to dashboard Cite

Histological study of the role of CD34+ stem cells and mast cells in cyclophosphamide-induced thymic injury in rats and the possible attenuating role of melatonin

Cited by 3 publications

References 40 publications

Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency rats

Therapeutic effect of notoginseng saponins before and after fermentation on blood deficiency rats

The protective effect of hydroalcoholic extract of Ephedra pachyclada leaves on ovarian damage induced by cyclophosphamide in rat: An experimental study

Anti-allergic drug screening in single living mast cells by means of a novel surface plasmon resonance imaging system

Contact Info

Product

Resources

About