Ultra-thin, aligned, free-standing nanofiber membranes to recapitulate multi-layered blood vessel/tissue interface for leukocyte infiltration study

Park, Sang Min; Kim, Hye Mi; Song, Kwang Hoon; Eom, Seong Su; Park, Hyoung Jun; Doh, Jun Sang; Kim, Dong Sung

doi:10.1016/j.biomaterials.2018.03.053

Cited by 41 publications

(29 citation statements)

References 49 publications

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“…The recent studies in our group revealed that the electrolyte solution collector served as the metal collector in controlling the distribution of electric field. [ 19–21 ] In the present study, the combination of metal and electrolyte solution as the grounded collectors allowed flexibly changing the electric field distribution considering the fluidic nature of the electrolyte solution to be suitable for each aligned and random nanofiber deposition.…”

Section: Resultsmentioning

confidence: 99%

“…For the measurement, four different electrospun bilayer membranes, including M10E1, M10E3, M10E5, and M10E10, were prepared. The fabricated electrospun bilayer membrane was dried at room temperature and immersed in an uncured mixture of PDMS base and curing agent in a weight ratio of 10:1 (Sylgard 184; Dow Corning, USA) followed by baking at 40 °C for 12 h. [ 21 ] The sample was cut vertically by a razor blade, and the cross‐sectional images of the electrospun bilayer membrane were obtained using an optical microscope (Eclipse 80i, Nikon, Japan). The average thickness of each membrane was calculated from the thickness measurements on ten different locations of the sample.…”

Section: Methodsmentioning

confidence: 99%

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Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Han

Hong

Park

et al. 2020

Adv Materials Inter

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vivo extracellular matrix (ECM). [2] Various advantages of the electrospun nanofiber membranes, including broad material selection, a high surface-area-to-volume ratio, controllability of physical properties (e.g., diameter and orientation of the nanofibers and porosity and thickness of the membrane), and capability in fabrication of complex nanostructures (e.g., coreshell and Janus nanofibers), [3] have made remarkable advancements in the development of in vitro tissue models and in vivo tissue regeneration. [1,2] Many studies have tried to control the spatial orientation of the electrospun nanofibers through electrospinning, given that the cellular activities, such as adhesion, migration, proliferation, and differentiation, could be promoted by the contact guidance through an anisotropic topographical cue of the electrospun nanofibers. Among various approaches to control the orientation of the electrospun nanofibers, including the electric fieldassisted method, rotating drum method, and magnetic electrospinning, [4-6] the electric field-assisted method readily modulates the orientation of the electrospun nanofibers in a precise and versatile manner. Based on the electric field-assisted method, previous studies have achieved various types of membranes from a uniaxially aligned nanofiber membrane to radially, circumferentially, and biaxially aligned nanofiber membranes, [6-8] showing their potential in manipulating many cell functions, such as myogenic differentiation of myoblast, dural fibroblast migration, and tight junction formation of endothelial cells. [9-11] However, the aligned nanofiber membrane produced by the electric field-assisted method is mechanically unstable, in general, because of its thin (<30 µm thick), anisotropic, and sparsely interconnected nanofibrous structures. [12-14] Hence, the aligned nanofiber membrane is prone to be damaged by external forces occurred with the experimental and surgical manipulations and the aqueous conditions during in vitro and in vivo biomedical applications. [9,15,16] An innovative approach to overcome such limitations of the aligned nanofiber membranes is to develop an electrospun bilayer membrane, also known as a kind of Janus sheet, composed of two membranes, one aligned and the other random, which simultaneously provide the anisotropic topographical Electrospun bilayer membranes comprising two layers, one aligned and the other random, have shown great potential in tissue engineering but previous fabrication processes inevitably relied on manual integration and produced limited types of membranes. Here, a metal-electrolyte solution dual-mode electrospinning (M-ELES) for fabrication of electrospun bilayer membrane based on a metal-electrolyte solution switchable collector is developed. The switchable collector enables random nanofiber deposition directly over the preexisting aligned nanofiber layer in an in situ manner and integration of the layers through an on-demand switch from the metal to the electrolyte solution collector. The electrolyte solution can eff...

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Section: Resultsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Han

Hong

Park

et al. 2020

Adv Materials Inter

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“…This method can be applied to a broad range of materials including inorganic materials [14], natural and synthetic polymers [15] and composite nanomaterials [16] with final products having large surface areas and a higher aspect ratio. Furthermore, by controlling process parameters including, starting materials, applied voltage, feed rate, working distance or collector type, various morphologies and network structures such as porous [17], core-shell [18], hollow [19] or aligned nanofibers [20] can be generated.…”

Section: Introductionmentioning

confidence: 99%

On the Nanoscale Mapping of the Mechanical and Piezoelectric Properties of Poly (L-Lactic Acid) Electrospun Nanofibers

et al. 2020

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The effect of the post-annealing process on different properties of poly (L-lactic acid) (PLLA) nanofibers has been investigated in view of their use in energy-harvesting devices. Polymeric PLLA nanofibers were prepared by using electrospinning and then were thermally treated above their glass transition. A detailed comparison between as-spun (amorphous) and annealed (semi-crystalline) samples was performed in terms of the crystallinity, morphology and mechanical as well as piezoelectric properties using a multi-technique approach combining DSC, XRD, FTIR, and AFM measurements. A significant increase in the crystallinity of PLLA nanofibers has been observed after the post-annealing process, together with a major improvement of the mechanical and piezoelectric properties.

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“…By modulating the chemical and topographical properties of an electrospun nanofiber membrane, the biochemical and/or biophysical cues can be implemented in an in vitro cell culture platform 22–24 . Various methods, including gel coating, surface modification, and blending hydrogel/polymer, have been developed for the incorporation of natural hydrogels, such as collagen and gelatin, into nanofiber membranes for the purpose of promoting EC proliferation and endothelial barrier formation through biochemical cues 23–27 . Electrospinning can control surface topography to facilitate the construction of endothelial barrier by modulating the diameter and alignment of nanofibers 21,28–31 .…”

Section: Introductionmentioning

confidence: 99%

A collagen gel-coated, aligned nanofiber membrane for enhanced endothelial barrier function

Kim

Eom

Park

et al. 2019

Sci Rep

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Herein, a collagen gel-coated and aligned nanofiber membrane named Col-ANM is developed, which remarkably improves endothelial barrier function by providing biochemical and topographical cues simultaneously. Col-ANM is fabricated by collagen gel coating process on an aligned polycaprolactone (PCL) nanofiber membrane, which is obtained by a simple electrospinning process adopting a parallel electrode collector. Human umbilical vein endothelial cells (HUVECs) cultured on Col-ANM exhibit remarkably enhanced endothelial barrier function with high expression levels of intercellular junction proteins of ZO-1 and VE-cadherin, a high TEER, and a cellular permeability compared with the artificial porous membranes in commercial cell culture well inserts. The enhanced endothelial barrier function is conjectured to be attributed to the synergistic effects of topographical and biochemical cues provided by the aligned PCL nanofibers and collagen gel in the Col-ANM, respectively. Finally, the reactive oxygen species is applied to the HUVEC monolayer formed on the Col-ANM to destroy the tight junctions between HUVECs. The destruction of the tight junctions is demonstrated by the decreased TEER value over time. Results indicate the potential of Col-ANM in modeling endothelial barrier dysfunction-related diseases.

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Ultra-thin, aligned, free-standing nanofiber membranes to recapitulate multi-layered blood vessel/tissue interface for leukocyte infiltration study

Cited by 41 publications

References 49 publications

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

Metal–Electrolyte Solution Dual‐Mode Electrospinning Process for In Situ Fabrication of Electrospun Bilayer Membrane

On the Nanoscale Mapping of the Mechanical and Piezoelectric Properties of Poly (L-Lactic Acid) Electrospun Nanofibers

A collagen gel-coated, aligned nanofiber membrane for enhanced endothelial barrier function

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