A collagen gel-coated, aligned nanofiber membrane for enhanced endothelial barrier function

Kim, Dohui; Eom, Seongsu; Park, Sang Min; Hong, Hyunsuk

doi:10.1038/s41598-019-51560-8

Cited by 47 publications

(34 citation statements)

References 47 publications

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“…In the literature, different approaches can be found to reach this goal. Often a high-speed rotating collector is used [21][22][23], but fiber orientation can also be reached by using a pair of blades as the collector [24,25] or stretching the electrospun mat in ethanol [2].…”

Section: Introductionmentioning

confidence: 99%

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

et al. 2019

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Electrospinning can be used to create nanofibers from diverse polymers in which also other materials can be embedded. Inclusion of magnetic nanoparticles, for example, results in preparation of magnetic nanofibers which are usually isotropically distributed on the substrate. One method to create a preferred direction is using a spinning cylinder as the substrate, which is not always possible, especially in commercial electrospinning machines. Here, another simple technique to partly align magnetic nanofibers is investigated. Since electrospinning works in a strong electric field and the fibers thus carry charges when landing on the substrate, using partly conductive substrates leads to a current flow through the conductive parts of the substrate which, according to Ampère’s right-hand grip rule, creates a magnetic field around it. We observed that this magnetic field, on the other hand, can partly align magnetic nanofibers perpendicular to the borders of the current flow conductor. We report on the first observations of electrospinning magnetic nanofibers on partly conductive substrates with some of the conductive areas additionally being grounded, resulting in partly oriented magnetic nanofibers.

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Section: Introductionmentioning

confidence: 99%

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

et al. 2019

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“…9 The biological properties of polymeric nanofibrous scaffolds can be improved by modifying their surface with biocompatible and bioactive materials. [10][11][12] Several works have used bioceramics to cover the surface of nanofibers to simultaneously benefit from their advantageous. These bioceramic-coated nanofibers have been shown to improve the process of osteogenesis in vitro and in vivo.…”

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confidence: 99%

Electrospun poly(l‐lactide) nanofibers coated with mineral trioxide aggregate enhance odontogenic differentiation of dental pulp stem cells

Sanaei‐rad

Jamshidi

Adel

et al. 2020

Polymers for Advanced Techs

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A combination of bioceramics and nanofibrous scaffolds holds promising potential for inducing of mineralization in connective tissues. The aim of the present study was to investigate the attachment, proliferation and odontogenic differentiation of dental pulp stem cells (DPSC) on poly(L-lactide) (PLLA) nanofibers coated with mineral trioxide aggregate (MTA). Polymeric scaffolds were fabricated via the electrospinning method and their surface was coated with MTA. DPSC were isolated from dental pulp and their biological behavior was evaluated on scaffolds and the control group using MTT assay. Alkaline phosphatase (ALP) activity, biomineralization and the expression of odontogenic genes were analyzed during odontogenic differentiation. Isolated DPSC showed spindle-shaped morphology with multi-lineage differentiation potential and were positive for CD73, CD90 and CD105. MTA-coated PLLA (PLLA/MTA) exhibited nanofibrous structure with average fiber diameter of 756 ± 157 nm and interconnected pores and also suitable mechanical properties. Similar to MTA, these scaffolds were shown to be biocompatible and to support the attachment and proliferation of DPSC. ALP activity transiently peaked on day 14 and was significantly higher in PLLA/MTA scaffolds than in the control groups. In addition, increasing biomineralization was observed in all groups with a higher amount in PLLA/MTA. Odontogenic-related genes, DSPP and collagen type I showed a higher expression in PLLA/MTA on days 21 and 14, respectively. Taken together, MTA/PLLA electrospun nanofibers enhanced the odontogenic differentiation of DPSC and showed the desired characteristics of a pulp capping material.

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“…Apparently, the substrate has therefore no discernible influence on the fiber diameter. On the other hand, partially conductive substrate areas can influence the orientation of electrospun nanofibers [13][14][15]. Figure 5 depicts the FFT analysis of the HIM images shown in Figure 2, as well as the HIM images color-coded according to the fiber orientations.…”

Section: Resultsmentioning

confidence: 99%

“…In previous studies, we have shown that DSSCs can be purely electrospun [11], and have investigated the influence of different foil materials on DSSC properties [12]. The main objective of this study is to investigate the adhesion of nanofibers directly electrospun onto different substrates and the influence of the conductive substrate on fiber diameter and orientation [13][14][15]. The combination of conductive foils, which contain carbon materials in particular and can thus be used as counter electrodes, with insulating nanofiber mats is highly interesting, since the latter can be used to hold liquid electrolytes [11].…”

Section: Introductionmentioning

confidence: 99%

Adhesion of Electrospun Poly(acrylonitrile) Nanofibers on Conductive and Isolating Foil Substrates

et al. 2021

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Electrospinning can be used to prepare nanofibers from various polymers and polymer blends. The adhesion of nanofibers to the substrates on which they are electrospun varies greatly with the substrate material and structure. In some cases, good adhesion is desired to produce sandwich structures by electrospinning one material directly onto another. This is the case, e.g., with dye-sensitized solar cells (DSSCs). While both pure foil DSSCs and pure electrospun DSSCs have been examined, a combination of both technologies can be used to combine their advantages, e.g., the lateral strength of foils with the large surface-to-volume ratio of electrospun nanofibers. Here, we investigate the morphology and adhesion of electrospun nanofibers on different foil substrates containing materials commonly used in DSSCs, such as graphite, poly(3,4-ethylenedioxythiophene) polystyrene sulfonate (PEDOT:PSS) or TiO2. The results show that the foil material strongly influences the adhesion, while a plasma pretreatment of the foils showed no significant effect. Moreover, it is well known that conductive substrates can alter the morphology of nanofiber mats, both at microscopic and macroscopic levels. However, these effects could not be observed in the current study.

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A collagen gel-coated, aligned nanofiber membrane for enhanced endothelial barrier function

Cited by 47 publications

References 47 publications

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

Orientation of Electrospun Magnetic Nanofibers Near Conductive Areas

Electrospun poly(l‐lactide) nanofibers coated with mineral trioxide aggregate enhance odontogenic differentiation of dental pulp stem cells

Adhesion of Electrospun Poly(acrylonitrile) Nanofibers on Conductive and Isolating Foil Substrates

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