Electrospun poly(<scp>l</scp>‐lactide) nanofibers coated with mineral trioxide aggregate enhance odontogenic differentiation of dental pulp stem cells

Sanaei‐rad, Parisa; Jamshidi, Davoud; Adel, Milad; Seyedjafari, Ehsan

doi:10.1002/pat.5095

Cited by 11 publications

(11 citation statements)

References 33 publications

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“…This data indicated that DPSCs grown on PLCL scaffold could be differentiated into cells showing an osteoblast immunophenotype and secrete a mineralized bone-like matrix. In line with other reports, we might conclude that PLCL nonfibrous scaffold provided a favorable microenvironment for the intracellular signaling activity in cell–cell and cell–extracellular matrix interactions and affected osteogenic differentiation of hDPSCs and mineralization of ECM [ 45 , 46 ]. Based on the presented results, bioconstruct designed of adherent hDPSCs to PLCL nonfibrous scaffold showing high proliferation, osteogenic potency and mineralization ability seems to be a promise graft materials for bone tissue engineering [ 39 ].…”

Section: Discussionsupporting

confidence: 92%

“…However, a pure PLCL scaffold is hydrophobic, and some modifications are needed to improve its physicomechanical properties [ 42 , 44 , 45 ]. Recently, several reports found that the modification of electrospun poly( l -lactide) or poly(ε-caprolactone) nanofiber scaffolds improves hDPSCs osteogenic differentiation [ 5 , 39 , 46 ]. Although electrospun nanofibers scaffolds showed high potential in bone tissue engineering, different problems must be solved.…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Biological Properties of Dental Pulp Stem Cells Grown on an Electrospun Poly(l-lactide-co-caprolactone) Scaffold

et al. 2022

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Poly(L-lactide-co-caprolactone) (PLCL) electrospun scaffolds with seeded stem cells have drawn great interest in tissue engineering. This study investigated the biological behavior of human dental pulp stem cells (hDPSCs) grown on a hydrolytically-modified PLCL nanofiber scaffold. The hDPSCs were seeded on PLCL, and their biological features such as viability, proliferation, adhesion, population doubling time, the immunophenotype of hDPSCs and osteogenic differentiation capacity were evaluated on scaffolds. The results showed that the PLCL scaffold significantly supported hDPSC viability/proliferation. The hDPSCs adhesion rate and spreading onto PLCL increased with time of culture. hDPSCs were able to migrate inside the PLCL electrospun scaffold after 7 days of seeding. No differences in morphology and immunophenotype of hDPSCs grown on PLCL and in flasks were observed. The mRNA levels of bone-related genes and their proteins were significantly higher in hDPSCs after osteogenic differentiation on PLCL compared with undifferentiated hDPSCs on PLCL. These results showed that the mechanical properties of a modified PLCL mat provide an appropriate environment that supports hDPSCs attachment, proliferation, migration and their osteogenic differentiation on the PLCL scaffold. The good PLCL biocompatibility with dental pulp stem cells indicates that this mat may be applied in designing a bioactive hDPSCs/PLCL construct for bone tissue engineering.

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Section: Discussionsupporting

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Characterization of Biological Properties of Dental Pulp Stem Cells Grown on an Electrospun Poly(l-lactide-co-caprolactone) Scaffold

et al. 2022

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“…There is a paradigm shift for endodontic therapy in dentistry from simple capping to dental tissue reconstruction because the effectiveness and durability of the use of specialized tissue-compatible materials in conventional dental therapies is still unclear [49]. Tissue engineering approaches based on cell-based, scaffolds based and scaffold-free cell-based strategies are used to discover the ideal treatment method and material that modulates tissue defence/repair and promotes dentinpulp complex regeneration [50,51].…”

Section: Conventional and Tissue Engineering-based Vital Pulp Treatmentmentioning

confidence: 99%

Approaches to vital pulp therapies

Ballıkaya

Çelebi‐Saltik

2023

Aust Endodontic J

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Tooth decay, which leads to pulpal inflammation due to the pulp’s response to bacterial components and byproducts is the most common infectious disease. The main goals of clinical management are to eliminate sources of infection, to facilitate healing by regulating inflammation indental tissue, and to replace lost tissues. A variety of novel approaches from tissue engineering based on stem cells, bioactive molecules, and extracellular matrix‐like scaffold structures to therapeutic applications, or a combination of all these are present in the literature. Shortcomings of existing conventional materials for pulp capping and the novel approches aiming to preserve pulp vitality highligted the need for developing new targeted dental materials. This review looks at the novel approches for vital pulp treatments after briefly addresing the conventional vital pulp treatment as well as the regenerative and self defense capabilities of the pulp. A narrative review focusing on the current and future approaches for pulp preservation was performed after surveying the relevant papers on vital pulp therapies including pulp capping, pulpotomy, and potential approaches for facilitating dentin‐pulp complex regeneration in PubMed, Medline, and Scopus databases.

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“…Tissue-engineering approaches appear highly promising for the regeneration of injured tissues [ 158 ]. In dentistry, we are looking forward to the technology that allows for the regeneration of tissues such as the periodontal ligament, enamel, dentin, and alveolar bone [ 159 , 160 ]. Tissue engineering combines three critical components: scaffolds, mesenchymal stem cells (MSCs), and growth factors, and seems to be an auspicious approach in dentistry [ 158 , 161 ].…”

Section: Potential Applications Of Hybrid Multifunctional Nanofibersmentioning

confidence: 99%

“…A scaffold must be designed with appropriate biocompatibility, biodegradability, architecture, and mechanical properties to promote the formation of a natural, extracellular matrix [ 158 , 161 , 162 ]. Recently, hybrid nanofibers, including coated nanofibers, have attracted the attention of investigators since they have shown to promote stem cells’ adhesion, growth, and differentiation into functional cells [ 160 , 161 , 162 ]. However, in vitro and in vivo experimental studies which described the biological effect of the nanofibrous scaffold with loaded MSCs in bone-tissue engineering are limited [ 163 , 164 , 165 ].…”

Section: Potential Applications Of Hybrid Multifunctional Nanofibersmentioning

confidence: 99%

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

et al. 2022

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Electrospun hybrid nanofibers, based on functional agents immobilized in polymeric matrix, possess a unique combination of collective properties. These are beneficial for a wide range of applications, which include theranostics, filtration, catalysis, and tissue engineering, among others. The combination of functional agents in a nanofiber matrix offer accessibility to multifunctional nanocompartments with significantly improved mechanical, electrical, and chemical properties, along with better biocompatibility and biodegradability. This review summarizes recent work performed for the fabrication, characterization, and optimization of different hybrid nanofibers containing varieties of functional agents, such as laser ablated inorganic nanoparticles (NPs), which include, for instance, gold nanoparticles (Au NPs) and titanium nitride nanoparticles (TiNPs), perovskites, drugs, growth factors, and smart, inorganic polymers. Biocompatible and biodegradable polymers such as chitosan, cellulose, and polycaprolactone are very promising macromolecules as a nanofiber matrix for immobilizing such functional agents. The assimilation of such polymeric matrices with functional agents that possess wide varieties of characteristics require a modified approach towards electrospinning techniques such as coelectrospinning and template spinning. Additional focus within this review is devoted to the state of the art for the implementations of these approaches as viable options for the achievement of multifunctional hybrid nanofibers. Finally, recent advances and challenges, in particular, mass fabrication and prospects of hybrid nanofibers for tissue engineering and biomedical applications have been summarized.

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Electrospun poly(l‐lactide) nanofibers coated with mineral trioxide aggregate enhance odontogenic differentiation of dental pulp stem cells

Cited by 11 publications

References 33 publications

Characterization of Biological Properties of Dental Pulp Stem Cells Grown on an Electrospun Poly(l-lactide-co-caprolactone) Scaffold

Characterization of Biological Properties of Dental Pulp Stem Cells Grown on an Electrospun Poly(l-lactide-co-caprolactone) Scaffold

Approaches to vital pulp therapies

Advances in Electrospun Hybrid Nanofibers for Biomedical Applications

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