2004
DOI: 10.1016/j.jchromb.2004.01.012
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Ultra sensitive method for the determination of 9-(2-phosphonylmethoxyethyl)adenine in human serum by liquid chromatography–tandem mass spectrometry

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Cited by 14 publications
(23 citation statements)
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References 13 publications
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“…Several chromatographic methods have been used for the determination of PMEA in biological samples including UV detection [9][10][11], fluorescence detection of derivatized PMEA [12][13][14], radiochromatography [15], and highly sensitive liquid chromatography-tandem mass spectrometry methods [16,17]. None of them, however, focused on the skin as the biological matrix.…”
Section: Introductionmentioning
confidence: 99%
“…Several chromatographic methods have been used for the determination of PMEA in biological samples including UV detection [9][10][11], fluorescence detection of derivatized PMEA [12][13][14], radiochromatography [15], and highly sensitive liquid chromatography-tandem mass spectrometry methods [16,17]. None of them, however, focused on the skin as the biological matrix.…”
Section: Introductionmentioning
confidence: 99%
“…The pretreatment of samples was fairly tedious and time-consuming, and the method was not sensitive enough to evaluate the pharmacokinetics of PMEA following the approved oral dose of ADV (10 mg once daily). Zhao et al [7] and Liu et al [8] developed a sensitive LC-MS/MS method for the determination of PMEA in human plasma and serum, with the internal standard (I.S.) 9[(R)-2-(phosphonomethoxy)propyl]adenine (PMPA) and [ 13 C]PMEA, respectively.…”
Section: Introductionmentioning
confidence: 99%
“…In the literature [9] a solid phase extraction (SPE) was applied to purify and enrich adefovir from human plasma, which would be expensive and not suitable for sample preparation in large numbers. Therefore, a procedure of protein precipitation combined with interference elimination using dichloromethane extraction and concentration by evaporation was chosen as the sample preparation method, which provided the same purification and concentration effect as SPE.…”
Section: Selection Of Extraction Methodsmentioning
confidence: 99%
“…Furthermore, low column back pressure originated from the use of high organic content mobile phase in the HILIC method makes it possible for the implementation of high flow rate, which can significantly shorten the analysis time. A chromatographic run time as short as 3.0 min per sample was about half of those reported in the literatures (6-8 min) [9][10][11][12]. In this study, a 1.7 m un-derivatized ethylene bridged hybrid (BEH) particle was employed under HILIC condition.…”
Section: Development Of Chromatographymentioning
confidence: 99%
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