Development and validation of a sensitive LC–MS/MS method for the determination of adefovir in human serum and urine

Sun, Deqing; Wang, Haisheng; Wang, Benjie; Guo, Ruru

doi:10.1016/j.jpba.2006.04.019

Cited by 19 publications

(17 citation statements)

References 7 publications

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“…After oral administration of single dose (10 mg) of ADV to patients with HBV, the mean maximum concentration (C max ) of drug in plasma was 18.4±6.26 ng/mL, and the steady urinary recovery was approximately 45.3% [5].…”

Section: Introductionmentioning

confidence: 99%

“…Adefovir ( Fig.1) has been measured by different methods such as UV/vis spectrophotometry [6], ion-pair LC with fluorescence detection [7], HPLC with MS/MS or UV detection [5,[8][9][10] and voltammetry [11]. Some of these methods involve many derivatization and pretreatment procedures such as plasma protein precipitation or LLE before final analysis which are very time consuming and the demand of expensive, sophisticated instrumentation and highly skilled personnel restrict their use in routine analysis.…”

Section: Introductionmentioning

confidence: 99%

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Water‐compatible molecularly imprinted polymer as a sorbent for the selective extraction and purification of adefovir from human serum and urine

Pourfarzib

Dinarvand

Akbari‐adergani

et al. 2015

J of Separation Science

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A molecularly imprinted polymer has been synthesized to specifically extract adefovir, an antiviral drug, from serum and urine by dispersive solid-phase extraction before high-performance liquid chromatography with UV analysis. The imprinted polymers were prepared by bulk polymerization by a noncovalent imprinting method that involved the use of adefovir (template molecule) and functional monomer (methacrylic acid) complex prior to polymerization, ethylene glycol dimethacrylate as cross-linker, and chloroform as porogen. Molecular recognition properties, binding capacity, and selectivity of the molecularly imprinted polymers were evaluated and the results show that the obtained polymers have high specific retention and enrichment for adefovir in aqueous medium. The new imprinted polymer was utilized as a molecular sorbent for the separation of adefovir from human serum and urine. The serum and urine extraction of adefovir by the molecularly imprinted polymer followed by high-performance liquid chromatography showed a linear calibration curve in the range of 20-100 μg/L with excellent precisions (2.5 and 2.8% for 50 μg/L), respectively. The limit of detection and limit of quantization were determined in serum (7.62 and 15.1 μg/L), and urine (5.45 and 16 μg/L). The recoveries for serum and urine samples were found to be 88.2-93.5 and 84.3-90.2%, respectively.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Water‐compatible molecularly imprinted polymer as a sorbent for the selective extraction and purification of adefovir from human serum and urine

Pourfarzib

Dinarvand

Akbari‐adergani

et al. 2015

J of Separation Science

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“…1b) in human serum and urine (22), and the HPLC method for the determination of a nucleoside analog acyclovir and its related impurities have also been reported (23). However, to our knowledge there is no HPLC method that has been reported so far for simple and rapid quantitative estimation of TNF in nanomedicines (NMs) formulation.…”

Section: Introductionmentioning

confidence: 99%

Sensitive and Rapid HPLC Quantification of Tenofovir from Hyaluronic Acid-Based Nanomedicine

Agrahari

Youan

2012

AAPS PharmSciTech

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Abstract. The purpose of this study was to develop and validate a rapid, sensitive, and specific reversedphase high-performance liquid chromatography method for the quantitative determination of native tenofovir (TNF) for various applications. Different analytical performance parameters such as linearity, precision, accuracy, limit of quantification (LOQ), limit of detection (LOD), and robustness were determined according to International Conference on Harmonization (ICH) guidelines. A Bridge™ C18 column (150×4.6 mm, 5 μm) was used as stationary phase. The retention time of TNF was 1.54±0.03 min (n=6). The assay was linear over the concentration range of 0.1-10 μg/mL. The proposed method was sensitive with LOD and LOQ values equal to 50 and 100 ng/mL, respectively. The method was accurate with percent mean recovery from 95.41% to 102.90% and precise as percent RSD (relative standard deviation) values for intra-day, and inter-day precision were less than 2%. This method was utilized for the estimation of molar absorptivity of TNF at 259 nm (ε 259 =12,518 L/mol/cm), calculated from linear regression analysis. The method was applied for determination of percentage of encapsulation efficiency (22.93±0.04%), drug loading (12.25±1.03%), in vitro drug release profile in the presence of enzyme (43% release in the first 3 h) and purification analysis of hyaluronic acid-based nanomedicine.

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“…The pretreatment of samples was tedious and time-consuming, and those methods were not sensitive enough for evaluating the pharmacokinetics of adefovir dipivoxil with LLOQ higher than 10 ng/mL. Several sensitive highperformance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) methods [9][10][11][12] have been developed using electrospray ionization (ESI) for the measurement of adefovir in human plasma or serum. Although those methods were sensitive for the pharmacokinetic study of adefovir, long analysis time (longer than 8 min) precluded their use in therapeutic drug monitoring.…”

Section: Introductionmentioning

confidence: 99%

Hydrophilic interaction liquid chromatography–tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

Xiong

Zhang

Qin

et al. 2010

Journal of Chromatography B

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Development and validation of a sensitive LC–MS/MS method for the determination of adefovir in human serum and urine

Cited by 19 publications

References 7 publications

Water‐compatible molecularly imprinted polymer as a sorbent for the selective extraction and purification of adefovir from human serum and urine

Water‐compatible molecularly imprinted polymer as a sorbent for the selective extraction and purification of adefovir from human serum and urine

Sensitive and Rapid HPLC Quantification of Tenofovir from Hyaluronic Acid-Based Nanomedicine

Hydrophilic interaction liquid chromatography–tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

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