2010
DOI: 10.1016/j.jchromb.2010.06.024
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Hydrophilic interaction liquid chromatography–tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

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Cited by 19 publications
(8 citation statements)
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“…Several techniques have been explored for detection and quantification of Adefovir in pharmaceutical formulation and biological fluids such as spectrophotometry [5] , high performance liquid chromatography [6] , [7] , ion-pair liquid chromatography [8] , [9] , hydrophilic interaction liquid chromatography–tandem mass spectrometry [10] and liquid chromatography–tandem mass spectrometry [11] , [12] . Although spectrophotometry and chromatography are the most commonly employed techniques, but these involve many derivatization steps and extraction purification approaches prior to final analysis.…”
Section: Introductionmentioning
confidence: 99%
“…Several techniques have been explored for detection and quantification of Adefovir in pharmaceutical formulation and biological fluids such as spectrophotometry [5] , high performance liquid chromatography [6] , [7] , ion-pair liquid chromatography [8] , [9] , hydrophilic interaction liquid chromatography–tandem mass spectrometry [10] and liquid chromatography–tandem mass spectrometry [11] , [12] . Although spectrophotometry and chromatography are the most commonly employed techniques, but these involve many derivatization steps and extraction purification approaches prior to final analysis.…”
Section: Introductionmentioning
confidence: 99%
“…The signal to noise (S/N) ratio of curdione was higher with acetonitrile–water as the mobile phase than that with methanol–water. Since the ESI source with positive ionization mode was used in this study, volatile acids, such as acetic acid and formic acid, were added to improve the ionization of analytes, especially for the formation of the protonated molecule [M + H] + owing to their good volatility and solubility at high percentages of organic solvent (Xiong et al ., ). The effect of formic acid at 0.1 and 0.2% in both aqueous and organic phase on the response and peak shape of curdione was investigated, and 0.1% formic acid was found to give the better results.…”
Section: Resultsmentioning
confidence: 97%
“…Matrix effect is due to co‐elution of some components present in biological samples. These co‐eluted components may not give a signal in MRM of the target analyte, but can certainly decrease or increase the analyte response dramatically to affect the sensitivity, accuracy and precision of a method (Xiong et al ., ). Evaluation of matrix effect from the co‐eluting components on analyte ionization is necessary for an HPLC‐MS/MS method.…”
Section: Resultsmentioning
confidence: 99%
“…Severalanalyticalm ethodshavebeen presented for quantification of adefovirin humanp lasmaa nd serum samples.Some of them described HPLC methodsutilizing derivatization withchloroacetaldehyde using fluorescenced etector [3][4][5][6].Theirlimits of quantification weren ots ensitivee nough forpharmacokineticstudies and sample preparationsw eretime consuming. Recently,severalm ethodsbased on liquid chromatographyw ithpositivei on electrosprayionization coupled to atandem mass spectrometry (LC-MS/MS)d etection for the quantification of adefovirin humanp lasmahave been developed [7][8][9][10][11][12].Thesem ethodsarevery sensitivebut theyaren otavailable formost laboratoriesbecauseo ftheirs pecialty requirementand financialreasons.The presents tudydescribesarapid and validated HPLC method using amonolithicc olumn withUV detection,whiche nablest he determination of adefovir withg ood accuracyatlowdrugconcentrationsin plasmausing single-step extraction procedure. Separation wasperformed on areversed-phasemonolithiccolumn, whichh aslowers eparation impedancecomparing to the particulatepackings,and thereforeitallows easy optimizing chromatographicc onditionst oo btain desirable resolution in ashort time.…”
Section: Introductionmentioning
confidence: 99%