Hydrophilic interaction liquid chromatography–tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

Xiong, Zhili; Zhang, Yi; Qin, Feng; Qin, Tianyi; Yang, Shanglu; Li, Famei

doi:10.1016/j.jchromb.2010.06.024

Cited by 19 publications

(8 citation statements)

References 22 publications

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“…Several techniques have been explored for detection and quantification of Adefovir in pharmaceutical formulation and biological fluids such as spectrophotometry [5] , high performance liquid chromatography [6] , [7] , ion-pair liquid chromatography [8] , [9] , hydrophilic interaction liquid chromatography–tandem mass spectrometry [10] and liquid chromatography–tandem mass spectrometry [11] , [12] . Although spectrophotometry and chromatography are the most commonly employed techniques, but these involve many derivatization steps and extraction purification approaches prior to final analysis.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

Jain

Sharma

2012

Journal of Pharmaceutical Analysis

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Electrochemical reduction behavior of Adefovir was studied using Hanging Mercury Drop Electrode (HMDE) in Britton–Robinson (BR) buffer solution. Voltammetric study showed one well-defined reduction peak in the potential range −1.2 to −1.4 V (vs. Ag/AgCl) due to reduction of C=N bond of the imidazole ring. Solid-phase extraction and protein-precipitation techniques were employed for extraction of Adefovir from human plasma. The proposed method allows quantification of Adefovir in human plasma over the concentration range 0.50–5.00 μg/mL with the detection limit 0.17 μg/mL, whereas in pharmaceutical formulation 0.25–2.25 μg/mL with the detection limit 0.08 μg/mL.

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Section: Introductionmentioning

confidence: 99%

Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

Jain

Sharma

2012

Journal of Pharmaceutical Analysis

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“…The signal to noise (S/N) ratio of curdione was higher with acetonitrile–water as the mobile phase than that with methanol–water. Since the ESI source with positive ionization mode was used in this study, volatile acids, such as acetic acid and formic acid, were added to improve the ionization of analytes, especially for the formation of the protonated molecule [M + H] + owing to their good volatility and solubility at high percentages of organic solvent (Xiong et al ., ). The effect of formic acid at 0.1 and 0.2% in both aqueous and organic phase on the response and peak shape of curdione was investigated, and 0.1% formic acid was found to give the better results.…”

Section: Resultsmentioning

confidence: 97%

“…Matrix effect is due to co‐elution of some components present in biological samples. These co‐eluted components may not give a signal in MRM of the target analyte, but can certainly decrease or increase the analyte response dramatically to affect the sensitivity, accuracy and precision of a method (Xiong et al ., ). Evaluation of matrix effect from the co‐eluting components on analyte ionization is necessary for an HPLC‐MS/MS method.…”

Section: Resultsmentioning

confidence: 99%

A validated LC‐MS/MS assay for the quantitative determination of curdione in rabbit plasma and its application to a pharmacokinetic study after administration of zedoary turmeric oil and bioavailability of the oil

Peng

Zhang

et al. 2014

Biomedical Chromatography

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A selective and sensitive liquid chromatography tandem mass spectrometry method was developed for the first time for the identification and quantification of curdione in rabbit plasma after vaginal drug administration and intravenous administration of zedoary turmeric oil (ZTO) solution (10 mg/kg). The analysis was performed on a triple-quadrupole tandem mass spectrometer with multiple reaction monitoring mode via electrospray ionization source in positive ionization mode. After mixing with internal standard diazepam, plasma samples were extracted with ethyl ether-acetic ether (1:1, v/v). Chromatographic separation was carried out on a C18 column with gradient elution using a mixture of water and acetonitrile (both containing 0.1% formic acid) as mobile phases. Linearity ranged over 1.06-106 and 10.6-530 ng/mL (r ≥ 0.995) with the lower limit of quantfication 1.06 ng/mL. The intra- and inter-day precision relative standard deviation values were <12% and the accuracy relative error was from -10.6 to -6.1% at all quality control sample levels. The method was applied to a study of the pharmacokinetics of curdione after vaginal drug administration and intravenous administration of ZTO.

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“…Severalanalyticalm ethodshavebeen presented for quantification of adefovirin humanp lasmaa nd serum samples.Some of them described HPLC methodsutilizing derivatization withchloroacetaldehyde using fluorescenced etector [3][4][5][6].Theirlimits of quantification weren ots ensitivee nough forpharmacokineticstudies and sample preparationsw eretime consuming. Recently,severalm ethodsbased on liquid chromatographyw ithpositivei on electrosprayionization coupled to atandem mass spectrometry (LC-MS/MS)d etection for the quantification of adefovirin humanp lasmahave been developed [7][8][9][10][11][12].Thesem ethodsarevery sensitivebut theyaren otavailable formost laboratoriesbecauseo ftheirs pecialty requirementand financialreasons.The presents tudydescribesarapid and validated HPLC method using amonolithicc olumn withUV detection,whiche nablest he determination of adefovir withg ood accuracyatlowdrugconcentrationsin plasmausing single-step extraction procedure. Separation wasperformed on areversed-phasemonolithiccolumn, whichh aslowers eparation impedancecomparing to the particulatepackings,and thereforeitallows easy optimizing chromatographicc onditionst oo btain desirable resolution in ashort time.…”

Section: Introductionmentioning

confidence: 99%

Rapid high-performance liquid chromatographic method for determination of adefovir in plasma using UV detection: application to pharmacokinetic studies

Mohsen

Zarghi

Shafaati

et al. 2011

Arzneimittelforschung

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A rapid, sensitive and reproducible HPLC method was developed and validated for the analysis of adefovir (CAS 106941-25-7) in human plasma. The separation was achieved on a monolithic silica column (Chromolith Performance RP-18e, 10064.6 mm) using acetonitrile-ammoniumdihydrogen phosphate buffer (6: 94, v/v), pH 5.2, as the mobile phase at a flow rate of 1.5 ml min?1. The wavelength was set at 260 nm. The assay enables the measurement of adefovir for therapeutic drug monitoring with a minimum quantification limit of 1 ng ml?1. The method involves a simple protein precipitation procedure. Analytical recovery was complete. The calibration curve was linear over the concentration range 1?40 ng ml?1. The coefficients of variation for inter-day and intra-day assay were found to be less than 5%. The method was applied to the determination of adefovir in plasma from 12 subjects dosed with adefovir 2610 mg tablets and pharmacokinetic parameters were evaluated.

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Hydrophilic interaction liquid chromatography–tandem mass spectrometry for the determination of adefovir in human plasma and its application to a pharmacokinetic study

Cited by 19 publications

References 22 publications

Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

Voltammetric quantification of anti-hepatitis drug Adefovir in biological matrix and pharmaceutical formulation

A validated LC‐MS/MS assay for the quantitative determination of curdione in rabbit plasma and its application to a pharmacokinetic study after administration of zedoary turmeric oil and bioavailability of the oil

Rapid high-performance liquid chromatographic method for determination of adefovir in plasma using UV detection: application to pharmacokinetic studies

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