Ultra high field NMR spectroscopic studies on human seminal fluid, seminal vesicle and prostatic secretions

Seminal Oligouridinosis: Low Uridine Secretion as a Biomarker for Infertility in Spinal Neurotrauma

“…1A) showed 2 doublets in the spectra (␦5.9, 3 J HH ϭ 4.41 Hz and ␦5.92, 3 J HH ϭ 8.01 Hz) that were absent from all but 2 of the spectra from SCI patients. These peaks are known from previous assignment to correspond to uridine (6 ). This NMR assignment was confirmed by spiking in a known amount of uridine.…”

Identification of biochemical differences between different forms of male infertility by nuclear magnetic resonance (NMR) spectroscopy

“…Resonances from a number of low molecular weight metabolites can be identified immediately from previously assigned chemical shift, such as choline, glycerophosphocholine (GPC), citrate, fructose, lactate and resonances from amino acids such as alanine, valine, leucine and isoleucine and is presented in Fig. 1 [22]. In addition, broad bands, characteristic of proteins, have also been observed throughout the entire spectrum, the assignment of which is hindered due to broadening.…”

“…There has been growing interest in the field of metabolomics which provides useful information about the phenotype an organism through analysis of various biomarkers in body fluids [22,35,39,46,37,7]. This technology provides for assessment of metabolic changes within an organism, even when standard clinical chemistry markers are within normal limits [31].…”

Nuclear magnetic resonance studies of boar seminal plasma. Problems encountered in the identification of small molecules: hypotaurine and carnitine

“…On the other hand, 13 C NMR spectroscopy demands high sensitivity which, apart from a high magnetic field, can be best obtained by proton-detected chemical shift correlation techniques. 5 The different requirements and methods for the answer to the questions raised by these nuclei were recently discussed by Lynch et al 6 in relation to NMR studies on human seminal fluid.…”

“…Although instruments with the highest magnetic fields and numerous modern NMR techniques are available, [5][6][7] it was clear from our previous study 1 on boar seminal plasma that it is by no means simple to identify all the substances in a logical way, especially those with low molecular weight which give relatively little information from the 1 H and 13 C NMR spectra. Although knowledge of the chemical shifts, chemical shift correlations and coupling constants was available for a particular compound (designated as X in Ref.…”