Uji Resistensi Bakteri Escherichia Coli Yang Diisolasi Dari Plak Gigi Terhadap Merkuri Dan Antibiotik Kloramfenikol

Dian, Ronal; Fatimawali, Fatimawali; Budiarso, Fona

doi:10.35790/ebm.3.1.2015.6607

Cited by 12 publications

(11 citation statements)

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“…Chloramphenicol is a broad‐spectrum antibiotic that effectively inhibits the growth of Gram‐positive or Gram‐negative bacteria, as well as anaerobic bacteria. (Dian & Budiarso, 2015). The results of the activity test showed that palm waste extracts generally have excellent antibacterial inhibition potential almost the same as the positive control (Chloramfenikol), which is a very strong category.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus

Nasmia,

Serdiati,

Tahya

et al. 2024

Journal of Fish Diseases

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Vibrio harveyi and Vibrio parahaemolyticus are species of the Vibrio genus that often cause disease and mass mortality in crustaceans. If not handled quickly and appropriately, these diseases can cause considerable losses to farmers. Therefore, it is necessary to find a solution with safe and environmentally friendly disease prevention technology using natural ingredients, among others from plants, namely oil palm. Some parts of oil palm, namely leaves, fronds, fibres and oil palm pulp, which are palm waste, contain antibacterial compounds. This study aimed to assess the antibacterial activity of palm waste extracts, namely pulp, leaves, fronds and fibres using n‐hexane, ethyl acetate, chloroform, ethanol and water maceration solvents against pathogenic bacteria V. harveyi and V. parahaemolyticus, and identify active compounds contained in palm waste. The results of the research are expected to produce innovative and sustainable solutions to control diseases in shrimp farming, contribute to the development of a sustainable fishing industry and open up the potential for utilizing palm waste as a value‐added resource in the field of aquatic health. The results of observations on antibacterial activity tests and identifying the content of palm waste extract compounds were analysed descriptively displayed in the form of figures, tables and graphs. The results showed that palm waste extracts (pulp, leaves, fronds and fibres) with ethyl acetate and ethanol maceration solvents had very strong antibacterial potential, namely 20.14 ± 0.31 mm–25.52 ± 1.42 mm on V. harveyi bacteria and 20.41 ± 0.55 mm–25.00 ± 0.51 mm on V. parahaemolyticus bacteria. Palm extracts with n‐hexane (>20 mm) and chloroform solvents generally have strong category antibacterial potential (10–20 mm), and palm extracts in water solvents have medium category potential (5–10 mm) against V. harveyi and V. parahemolyticus bacteria. The results of phytochemical tests on palm waste extracts with ethyl acetate and ethanol maceration solvents contain bioactive compounds of flavonoids, saponins, polyphenols and alkaloid tannins, steroids and triterpenoids. Palm extracts with n‐hexane and chloroform solvents generally contain saponins, alkaloids, steroids and triterpenoids, while palm waste extracts with water solvents contain saponins.

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Section: Discussionmentioning

confidence: 99%

Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus

Nasmia,

Serdiati,

Tahya

et al. 2024

Journal of Fish Diseases

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“…In contrast, chloramphenicol is positive control, which is a broad-spectrum antibacterial that inhibits both Gram-positive and Gram-negative. It is bacteriostatic at a low dose and bactericidal at high dose [ 30 ]. DMSO 10% is used because of this solvent's nature to dissolve polar and nonpolar compounds, where with a concentration of 10%, DMSO cannot inhibit cell growth in bacteria [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

Suppression of Proinflammatory Cytokines by Etlingera alba (A.D.) Poulsen Rhizome Extract and Its Antibacterial Properties

Hamsidi

Wahyuni

Sahidin

et al. 2021

Advances in Pharmacological and Pharmaceutical Sciences

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Etlingera alba is one of the Etlingera plants that has not been studied intensively. Plants that belong to the same genus have similar constituents and pharmacological activities. Thus, we aim to investigate the chemical composition and pharmacological activities, namely, anti-inflammatory and antibacterial properties, of E. alba rhizome extract (EA). The chemical constituent was detected using the test tube method. The inflammatory model rats were obtained by inducing them with 1% carrageenan, and their palm edema volume and cytokine levels, namely, IL-6, IL-12, and TNF-α, were measured. Antibacterial activity was performed with broth microdilution. The phytochemical screening of EA was detecting alkaloids, flavonoids, tannins, steroids, and phenols. The EA has anti-inflammatory activity by reducing the palms’ edema volume and cytokine levels (IL-6, IL-12, and TNF-α), and the optimal concentration was 400 mg/kg body weight (BW). On the other hand, EA also exhibited antibacterial properties against E. coli and S. enterica. In conclusion, similar to other Etlingera plants, EA also demonstrates pharmacological activities, namely, anti-inflammatory and antibacterial properties.

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“…Resistensi antibiotik bakteri patogen telah menjadi masalah di seluruh dunia. Terjadinya resistensi antibiotik tersebut disebabkan pemakaian antibiotik yang tidak tepat baik untuk pengobatan pada manusia maupun hewan (Indana and Effendi, 2020). Menurut data statistik, konsumsi antibiotik secara global pada tahun 2000 -2010 meningkat sekitar 36%.…”

Section: Pendahuluanunclassified

Keragaman dan Resistensi Antibiotik Isolat Bakteri Tanah di Dalam dan Luar TPU Bonoloyo, Surakarta Jawa Tengah

Rahmawati,

Rahayu,

Tyastuti

et al. 2023

Pharmacon

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A cemetery acts as an active decomposition site and contains bacteria which most likely have antibiotic resistance. However, related data to the phenomenon is limited. This study aimed to compare the diversity of bacteria inside and outside the cemetery and examine bacterial antibiotic resistance. First, samples were collected from three different spots inside and outside the Bonoloyo public cemetery in Surakarta, Central Java. Then, the bacteria were isolated by spread plate method on NA medium and were observed using Gram staining after 48 hours of incubation. Also, bacterial resistance against cefepime, bacitracine, and ampicilin were examined. As the results, bacterial populations inside and outside the cemetery area are 3.4x106 and 4.6x106 CFU/gram, respectively, with a diversity of 38% and 34%, while 28% of isolates are collected from both areas. The result of morphological observation showed that 15 and 21 isolates are respectively round and irregular, white to yellow colour with raised (16 isolates) and flat (19 isolates) elevation. Gram staining showed 48 isolates are coccus and 2 are bacilli, with 23 isolates of Gram negative and 27 isolates of Gram positive. Bacterial isolates showed resistance against cefepime (50%-50%) and against bacitracine (52%-43%) each from both areas of the cemetery, while resistance against ampicilin was relatively low (33%-21%). The number of bacterial populations and bacterial diversity inside and outside the cemetery area are not significantly different. In fact, the resistance of the soil bacteria population collected from cemetery soil are higher than that collected from soil outside the cemetery.

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Uji Resistensi Bakteri Escherichia Coli Yang Diisolasi Dari Plak Gigi Terhadap Merkuri Dan Antibiotik Kloramfenikol

Cited by 12 publications

References 0 publications

Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus

Phytochemical analysis and antibacterial activity of palm waste extract against Vibrio harveyi and Vibrio parahaemolyticus

Suppression of Proinflammatory Cytokines by Etlingera alba (A.D.) Poulsen Rhizome Extract and Its Antibacterial Properties

Keragaman dan Resistensi Antibiotik Isolat Bakteri Tanah di Dalam dan Luar TPU Bonoloyo, Surakarta Jawa Tengah

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