Uji Ketahanan Anggrek Hibrida Phalaenopsis terhadap Penyakit Busuk Lunak yang Disebabkan oleh Dickeya dadantii

Abstract

ABSTRACT<br /><br />One of the most popular orchids and cultivated by Indonesia and other countries is Phalaenopsis. The main disease of Phalaenopsis orchids in Indonesia is soft rot caused by bacteria Dickeya dadantii. The purpose of this study was to know the resistanctcy of Phalaenopsis hybrid to soft rot disease. The experiment was conducted at the Bacterial Plant Laboratory, Department of Plant Protection, the Greenhouse of Leuwikopo, Bogor Agricultural University, and the plastic house of Alam Sinar Sari Dramaga, Bogor from June 2014 to February 2015. The experimental design was randomized block design with three replications. Five genotypes of hybrid Phalaenopsis were tested, namely Phal. KHM 205, Phal. KHM 1126, Phal. KHM 1318, Phal. AMP 17, and Phal. KHM 2249. Phal. amabilis, Phal. esmeralda, Phal. amboinensis, and Phal. cornu-cervi were used as controls. The resistance testing was performed by inoculating bacteria D. dadantii on leaves of the orchids. The results showed all Phalaenopsis hybrid showed disease symptoms after inoculation. Phal. KHM 2249 had the lowest number of fallen leaves and the highest number of survive plants compared to the other hybrid Phalaenopsis. Survival rate was likely related to peroxidase activity and leaf thickness.<br /><br />Keywords: fallen leaves, leaf thickness, peroxidase, Phalaenopsis<br /><br />

Effect of genotype, concentration and timing of salicylic acid application to Phalaenopsis against Dickeya dadantii infection

“…All treatments showed the same pattern of AUPDC, and softrot symptom diameter, as shown in 'KHM 205', 1126, 1318, 2249. The result presented in this study confirmed that P. amboinensis was a more resistant genotype, as reported in the previous research (Firgiyanto et al 2016;Sukma et al 2017).…”

“…Exogenous SA concentration is a critical matter regulating its function as a plant hormone. The 10 ppm SA concentration induced resistance in Phalaenopsis hybrid KHM 205 but did not induce resistance in KHM 1138 against D. dadantii infection (Firgiyanto et al 2016). Induction of Phalaenopsis amabilis resistance using high SA concentrations (≤85 ppm) can suppress the growth of Fusarium oxysporum in vitro (Noviantia 2016).…”

“…Soft-rot symptom diameter (SD), disease severity (DS), resistance classes (RC) of Phalaenopsis genotypes against Dickeya dadantii infection after salicylic acid treatment and D. dadantii Colony forming unit on infected leaves The numbers in a column followed by similar letter are not significantly different in the Tukey test at α = 0.05; HPI: Hours Post-Inoculation; VS: Very Susceptible; MS: Moderate Susceptible; MR: Moderate Resistant Soft-rot symptom diameter (SD), disease severity (DS), resistance classes (RC) of Phalaenopsis amabilis leaves due to Dickeya dadantii infection after different timing of salicylic acid treatments and D. dadantii colony-forming unit on infected leaves ppm SA), almost all of the Phalaenopsis genotypes showed rapid development of soft-rot symptom, but it was slower in the Phalaenopsis 'Luzman' and PAM genotypes Sukma et al (2017),. andFirgiyanto et al (2016) reported PAM (P. amboinensis) to be the most resistant Phalaenopsis species against D. dadantii when compared to…”

Respon Plantlet In Vitro dan Induksi Ketahanan Bibit Phalaenopsis amabilis terhadap Dickeya dadantii Menggunakan Asam Salisilat

“…Pengendalian penyakit busuk lunak biasanya dilakukan dengan penyemprotan bakterisida, namun hal ini dapat mencemari lingkungan dan menurunkan kualitas tanaman karena sisa residu yang menempel pada permukaan tanaman (Firgiyanto et al, 2016). Penelitian ini dilakukan untuk mencari alternatif lain yang lebih ramah lingkungan dan efektif dalam mengendalikan penyakit busuk lunak antara lain dengan menginduksi ketahanan tanaman.…”

“…Analisis aktivitas peroksidase dilakukan mengikuti metode Sukma et al (2008) dan Firgiyanto et al (2016). Sampel yang digunakan yaitu bagian ujung daun bibit anggrek yang tidak terkena gejala busuk, dipotong dari masing-masing tanaman dan digabung sebagai sampel komposit untuk setiap konsentrasi perlakuan AS.…”