Effect of genotype, concentration and timing of salicylic acid application to Phalaenopsis against Dickeya dadantii infection

Abstract

Abstract. Sanjaya IPW, Sukma D, Sudarsono, Chan MT. 2020. Effect of genotype, concentration and timing of salicylic acid application to Phalaenopsis against Dickeya dadantii infection. Biodiversitas 21: 4317-4323. Soft-rot because of Dickeya dadantii (Dd) infection is one of the most difficult to control, affecting the cultivation of Phalaenopsis in tropical regions. Salicylic acid (SA) is known as an inducer of resistance against pathogens in some plant species. The study aim to identify the effects of genotypes, concentrations, and timing of salicylic acid application to Phalaenopsis against D. dadantii inoculation. In experiment 1, eight Phalaenopsis genotypes were treated by SA at doses of 0 and 60 ppm and then inoculated with D. dadantii. At a dose of 60 ppm, SA did not increase the resistance to D. dadantii in any genotypes. In experiment 2, a representative of a very susceptible genotype (P. amabilis) was treated with high SA concentrations (60, 360, and 720 ppm). The results demonstrated that at SA concentrations of 360 and 720 ppm, slightly increase P. amabilis resistance to D. dadantii. In experiment 3, the most effective SA concentration (720 ppm) was used at 2, 3, 5, or 7 days after SA treatment. The timing of the SA application did not affect the resistance of P. amabilis to D. dadantii. The present study shows that SA concentrations up to 60 ppm did not inhibit D. dadantii infection. Nevertheless, such inhibition was observed at a high SA dose (720 ppm) for a short period.

Analysis of the genetic diversity of Phalaenopsis orchids with single nucleotide polymorphisms and snap markers derived from the Pto gene

“…However, information about the actual role of R genes and resistance to pathogens in Phalaenopsis is limited. By contrast, the current study built upon early evidence regarding the response of Phalaenopsis species to infection by D. dadantii (Sukma et al, 2017;Sanjaya et al, 2020). Nonetheless, further research is required to dissect further the functional relationship between Pto and the response to D. dadantii in live plants.…”

Respon Plantlet In Vitro dan Induksi Ketahanan Bibit Phalaenopsis amabilis terhadap Dickeya dadantii Menggunakan Asam Salisilat

“…Pengamatan respon daun planlet P. amabilis terhadap infeksi bakteri penyebab penyakit busuk lunak dilakukan dengan mengukur diameter gejala busuk lunak setiap 6 jam sekali selama masa inkubasi dimulai dari 12 sampai 48 jam setelah inokulasi (JSI). Perhitungan keparahan penyakit (KP) dan kelas ketahanan (KK) planlet dihitung menggunakan rumus pada penelitian Sanjaya et al (2020). Keterangan : ni = jumlah daun terserang pada skor ke-1, v = skor penyakit pada skala i, Z = nilai skor tertinggi, N = jumlah tanaman yang diamati.…”

“…Planlet dengan kelas ketahanan tahan (T) tidak ditemukan sama sekali. Hasil ini menunjukkan bahwa secara umum P. amabilis tidak tahan terhadap D. dadantii, sesuai dengan hasil penelitian-penelitian yang dilakukan sebelumnya yang menyatakan anggrek P. amabilis merupakan spesies yang rentan terhadap penyakit busuk lunak (Sukma et al, 2017;Sanjaya et al, 2020). Respon ketahanan yang berbeda dari individu planlet menunjukkan kemungkinan adanya variasi genetik pada planlet walaupun benih dihasilkan dari penyerbukan sendiri, namun dugaan tersebut perlu dibuktikan lebih lanjut melalui analisis molekuler pada planlet.…”

“…Pengaruh konsentrasi asam salisilat (AS) terhadap diameter gejala, keparahan penyakit busuk lunak yang disebabkan D. dadantii dan kelas ketahanan bibit P. amabilis terhadap D. dadantii dengan intact leaf inoculation methodSanjaya et al (2020) melaporkan bahwa AS 60 ppm tidak meningkatkan ketahanan berbagai spesies Phalaenopsis terhadap D. dadantii, namun ketahanan sedikit meningkat dengan perlakuan AS 720 ppm pada P. amabilis. Konsistensi AS dalam menginduksi ketahanan tanaman anggrek Phalaenopsis terhadap penyakit busuk lunak yang disebabkan D. dadantii masih perlu diteliti lebih lanjut.Gambar 2.…”

Morphological, molecular and resistance responses to soft-rot disease variability among plantlets of Phalaenopsis amabilis regenerated from irradiated protocorms

“…Unfortunately, P. amabilis accession having such resistance mechanisms has never been identified. Most of the P. amabilis accessions from West Java, Indonesia, are very susceptible to soft-rot disease (Sukma et al 2017;Sanjaya et al 2020). The induced mutation has been used to widen orchid genetic diversity (Thinh et al 2011) while somaclonal variation arises among tissue culture regenerated plantlets (Kaeppler et al 2000;Bairu et al 2011;Wang and Wang 2012;Currais et al 2013;Ajijah et al 2016).…”