Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum Terhadap Rhyzoctonia solani, Sclerotium rolfsii dan Fusarium oxysporum secara in-vitro

Kalay, A. Marthin; Talahaturuson, Abraham; Rumahlewang, Wilhemina

doi:10.30598/a.v7i2.764

Cited by 7 publications

(7 citation statements)

References 8 publications

(10 reference statements)

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“…is a species of soil fungus known as a decomposer and biological controller of plant diseases. In vitro, T. harzianum inhibited the growth of soil-borne pathogens Fusarium oxysporum, Rhizoctonia solani, and Sclerotium rolfsii (Kalay et al, 2018). In this study, the Psolubilizing Fusarium fungus which is well known as a plant pathogen was obtained.…”

Section: Resultsmentioning

confidence: 89%

Abundance of Beneficial Soil Microbes in the Rhiosphere of Coffee Plants Infected by Pratylenchus coffeae

Hindersah,

Asyiah

2022

J Bio Bio Edu

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Enzymatic transformation in the cycle of nutrients of Nitrogen (N) and phosphorus (P) in soil can only be carried out by microbes. The latest approach in coffee cultivation is to utilize soil microbes to enhance plant growth, also to reduce the attack of the nematode Pratylenchus coffeae on coffee roots. This exploratory study aimed to observe the attack of P. coffeae and the presence of N-fixing bacteria (NFB) and P- solubilizing microbes (PSM) in the coffee rhizosphere which in turn have the potential to be used as biological fertilizers and bioprotectants of coffee plants. The study was conducted in PT Perkebunan Kalibendo's Arabica and Robusta coffee fields, East Java, on immature plants (IP) and mature plants (MP). This exploration explained that the IP and MP arabica and robusta coffee plantations were attacked by P. coffeae. NFB and PSM were successfully isolated from the rhizosphere of both coffee plantations types. The population of NFB Azotobacter in IP was lower than in MP, but the PSM population in IP rhizosphere was not different from that in MP. Based on morphological and biochemical characterization, three isolates of Azotobacter bacteria, six species of P- solubilizing bacteria (PSB) and six species of phosphate-solubilizing fungi (PSF) were obtained. This exploration confirmed that the microbes involved in the N and P cycle colonized the coffee rhizosphere which was attacked by P. coffeae. Further research is recommended to observe the effectiveness of microbes as biological fertilizers and bioprotectants for coffee plants.

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Section: Resultsmentioning

confidence: 89%

Abundance of Beneficial Soil Microbes in the Rhiosphere of Coffee Plants Infected by Pratylenchus coffeae

Hindersah,

Asyiah

2022

J Bio Bio Edu

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“…Keunggulan dari Trichoderma endofitik yang diisolasi dari akar dibandingkan dengan Trichoderma endofitik yang diisolasi dari batang dan daun karena Trichoderma adalah jamur yang kebanyakan hidup di tanah (soil). Jamur ini adalah bersifat antagonis untuk beberapa patogen jamur tanah antara lain Sclerotium rolfsii, Rhyzoctonia solani, Fusarium oxysporium [15] ., dan Phytopthora infestans [16] . Penurunan intensitas penyakit merupakan indikasi bahwa Trichoderma menekan perkembangan patogen penyebab penyakit (Phytopthora infestans).…”

Section: Intensitas Penyakitunclassified

Use of Endophytic Trichoderma to Control Fruit Rot Phytophthora infestans, Growth and Yield in Tomato

2021

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Endophytic Trichoderma is a fungus found in plant tissue and has the potential as a plant biological control agent. The aim of this study was to study endophytic Trichoderma isolated from tomato roots, stems and leaves to control Phytophthora infestans fruit rot and growth and yield of tomato plants. The treatments that were tested were endophytic tricoderma isolated from the roots, stems and leaves of tomato plants and applied with doses of 50, 75, and 100 g per tomato plant, respectively. The experimental design used a completely randomized design with three replications. The observed variables were disease intensity, plant height, number of flowers formed, and number of fruits formed. The results showed that the endophytic Trichoderma used could control the pod rot disease of Phytophthora infestans as well as increase the growth and yield of tomato plants. Giving endophytic Trichodema from roots applied with a dose of 100 g is the best treatment because it can reduce disease intensity by 48.02%, while giving at a dose of 75 g per plant is the best treatment because it can increase plant height by 51.74%, the number of flowers formed amounted to 68.42% and the number of fruit formed was 74.70%.Keywords: Endophytic Tricoderma, Tomato, Phytophthora infestans.

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“…Selanjutnya isolat BLJ5 persen hambatnya yaitu 90% terlihat dengan sedikitnya miselium S. rolfsi yang menyentuh bagian dari isolat selama 7 harihsi , dan isolat ALJ1 persen hambatnya yaitu 85% terlihat dengan mendominasinya miselium isolat ketimbang miselium jamur S. rolfsii. Besarnya persen hambat itu disebabkan oleh zat atau senyawa anti jamur yang dihasilkan oleh isolat ALJ3 yang dapat menghambat pertumbuhan fitopatogen melalui mekanisme aktivitas metabolit sekunder dan zat anti mikroba yang memiliki aktivitas antagonis (Kalay et al, 2018).…”

Section: Uji Antagonisme Isolat Jamur Endofit Vitex Trifolia L Terhadap Jamur Patogen Sclerotium Rolfsiiunclassified

Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

Basri

Zulkifli

Syukur

2021

JBT

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Plant damage by pathogenic fungi is often found in plants, one of which is caused by Sclerotium rolfsii. Biological control strategy offers a promising alternative for managing disease in plants because they are environmental friendly compared to pesticides application. One of the biological control offered is by using endophytic fungi isolated from Vitex trivolia L. The aim of the study was to isolate, to identify macroscopic and microscopic endophytic fungi from Vitex trifolia L and to test their antagonism potency against the pathogenic fungus Sclerotium rolfsii in vitro. The isolation obtained 7 endophytic fungi isolates identified based on their genus characteristics, nsmely Periconia sp, Aspergillussp, Dendrophoma sp, Geotrichum sp, Ampulliferina sp, Chalara sp, dan Bispora sp and 2 isolates have not been identified. The Antibacterial test of the fungi isolate on the 4 tested bacteria showed that of all the fungi isolate have low activity. The antagonism test using the direct opposition method with the PIRG formula, showed that the 3 isolates had high percentage of growth inhibition, in which ALJ1, BLJ5, and ALJ3 isolate has 85%, 90%, and 100% respectively. This potency could be used as biological agents on the pathogenic fungus Sclerotium rolfsii.

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Uji Antagonisme Trichoderma harzianum Dan Azotobacter chroococcum Terhadap Rhyzoctonia solani, Sclerotium rolfsii dan Fusarium oxysporum secara in-vitro

Cited by 7 publications

References 8 publications

Abundance of Beneficial Soil Microbes in the Rhiosphere of Coffee Plants Infected by Pratylenchus coffeae

Abundance of Beneficial Soil Microbes in the Rhiosphere of Coffee Plants Infected by Pratylenchus coffeae

Use of Endophytic Trichoderma to Control Fruit Rot Phytophthora infestans, Growth and Yield in Tomato

Isolation of Endophytic Fungi from Vitex trifolia L and Antagonism Test against Sclerotium rolfsii and pathogenic bacteria

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