Fusarium wilt caused by F. oxysporum f.sp. cubense is one of very damaging banana plant diseases which can cause plant death. Disease control using intensive chemical fungicides will have negative impacts on the environment and humans. Endophytic Trichoderma is one of the biological control agents which can reduce the amount of inoculum of pathogens, so it can reduce disease intensity. The objectives of this study was to assess the ability of endophytic Trichoderma in inducing plant resistance against fusarium wilt. Endophytic Trichoderma was obtained from healthy roots of banana from three regencies in Yogyakarta, namely Trichoderma harzianum.swn-1, T. harzianum.swn-2, T. harzianum.psr-1, T. asperrellum, T. gamsii, and T. koningiopsis. Research on induced resintance was conducted in the greenhouse with polybag using Completely Randomized Design with 14 treatments and 3 replications. The results showed that the ability of Trichoderma gamsii antagonism against F. oxysporum f.sp. cubense was 60.61%. T. asperellum and T. harzianum.swn-2 could suppress this disease resulted in disease intensity of 8.33% which categorize as resistant. Trichoderma harzianum.psr-1 was significantly different in stimulating plant vegetative growth. Induced resistance by using endophytic Trichoderma spp. against F. oxysporum f.sp. cubense showed increase in total phenolic compounds on the third and fourth weeks as well as peroxidase activity on the third, fourth and fifth weeks. Observation of lignification on the fifth week showed that lignification occurred in root xylem.
Endophytic Trichoderma is a fungus found in plant tissue and has the potential as a plant biological control agent. The aim of this study was to study endophytic Trichoderma isolated from tomato roots, stems and leaves to control Phytophthora infestans fruit rot and growth and yield of tomato plants. The treatments that were tested were endophytic tricoderma isolated from the roots, stems and leaves of tomato plants and applied with doses of 50, 75, and 100 g per tomato plant, respectively. The experimental design used a completely randomized design with three replications. The observed variables were disease intensity, plant height, number of flowers formed, and number of fruits formed. The results showed that the endophytic Trichoderma used could control the pod rot disease of Phytophthora infestans as well as increase the growth and yield of tomato plants. Giving endophytic Trichodema from roots applied with a dose of 100 g is the best treatment because it can reduce disease intensity by 48.02%, while giving at a dose of 75 g per plant is the best treatment because it can increase plant height by 51.74%, the number of flowers formed amounted to 68.42% and the number of fruit formed was 74.70%.Keywords: Endophytic Tricoderma, Tomato, Phytophthora infestans.
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