Über den Glykogengehalt des Scheidenepithels, seine diagnostische Bedeutung und deren kritische Bewertung

Identification of Body Fluid Stains Using Real-time RT-PCR: Discrimination Between Salivary, Nasal, and Vaginal Secretions

“…5,6,15 18) However, no speciˆc markers for nasal and vaginal secretions have been identiˆed, so identiˆcation of these secretions has relied on microscopic examination of nasal columnar epithelial cells and vaginal squamous cells, or immunologic analyses with speciˆc sera against human nasal and vaginal secretions. 19 22) Lugol's staining technique has long been used as a speciˆc test for vaginal squamous cells, 21,22) but is no longer considered su‹ciently speciˆc since glycogen-containing cells exist in male oral and urethral squamous cells. 23,24) In addition, most laboratories do not have speciˆc antibody reserves.…”

Immunohistochemical distribution of cyclic nucleotide phosphodiesterase (PDE) isoenzymes in the human vagina: A potential forensic value?

“…Especially, since the pioneer work of Wiegmann and Merkel, the staining procedure according to Lugol has been widely used in forensic daily practice as a simple and reliable method in order to allow the identification of vaginal epithelial cells, and is, thus, routinely utilized in the investigation of cases of sexual assaults. 1,2 However, due to the fact that Lugol-reactive polygonal cells are not exclusively limited to female genital organs but also found in for example, the male distal urethra, the specificity of this technique has been critically discussed and the use of other potential tissue markers proposed.…”