“…Altmann and Chayen (1965) showed that the loss of nitrogenous material from tissue sections is markedly reduced by the presence of PVA in the incubation medium. Arnold et al (1968) noted a considerable reduction in the diffusion of a soluble enzyme on the addition of PVA. With respect to the effect of PVA and polyvinyl pyrrolidon (PVP) on histochemical incubation, Dahl and Mellgren (1970) found that when tissue sections are incubated in the PVA-containing medium with PVP, the loss of lactic dehydrogenase by diffusion was minimal and negligible in practice.…”