Semipermeable Membrane Techniques in Quantitative Enzyme Histochemistry

Meijer, A. E. F. H.

doi:10.1002/9780470720561.ch7

Cited by 12 publications

(1 citation statement)

References 39 publications

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“…After euthanasia for morphological studies, fragments of the tibialis anterior muscle were taken from the local region at the level of its upper, middle and lower third. Transverse cryostat sections were made (microtomecryo stat M05050) to detect the activity of myosin-ATPase according to Padycula, Herman in the modification of A. E. Meijer [9] which allows you to identify different types of muscle fibers in muscles with different compositions. The preparations were digitized on the APK "DiaMorph" and morphometric using the Image program.…”

Section: Methodsmentioning

confidence: 99%

Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy

Yashchуshуn,

Dyadyk

2023

Pathologia

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The aim of this work was to reveal the effectiveness of delayed laser therapy for the regeneration of the sciatic nerve. Materials and methods. In 21 rats, transection and epineural microsurgical suture of the right sciatic nerve (SN) were performed. In 16 rats (experimental group) in the period from 1.0 to 2.5 months after surgery, a course of laser therapy (LT-1, n = 8) and a delayed (after 6 months) course of laser therapy (LT-2, n = 8) were used 18 sessions were performed in each subgroup. Laser therapy was not performed in 5 operated animals and they were included in the control group. The terms of withdrawal of rats from the experiment were 2.5 and 12.0 months after the operation. During the morphometry of semi-thin sections distal to the level of the sutures, the volumetric density of the constituent elements of the SN, the number density of myelinated nerve fibers (MNF), their diameters, the thickness of the axons and myelin sheath, the myelination index (index G) distribution of MNF by diameter and dependence of index G on the fiber caliber were measured. For double control, we used muscle morphometry data from intact rats (Intact). Muscle tissue was additionally examined by the method of transmission electron microscopy using a generally accepted technique with a REM-100 electron microscope (Selmi, Sumy, Ukraine). Results. In the control group, as compared to the intact group, the numerical density of muscle fibers was 21 % higher, but 38 % less than the numerical density of microvessels, therefore, the vascularization index was reduced by 1.7 times. The obtained results indicated laser compensation for denervation atrophy, which was confirmed by morphometric data. Conclusions. The results of the study indicate a stimulating prolonged effect of delayed laser therapy in regenerative axonogenesis.

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Section: Methodsmentioning

confidence: 99%

Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy

Yashchуshуn,

Dyadyk

2023

Pathologia

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Fluctuations in the enzymatic activity of the human endometrium

Elias¹,

Elias

Kooistra³

et al. 1983

Histochemistry

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Cyclic fluctuations were studied in the activity of oxidoreductases playing a role in the major energy metabolic pathways, lysosomal and non-lysosomal hydrolases and some non-enzymatic cytochemical components demonstrable in different developmental physiological or pathophysiological phases of human endometrium. The total scope of the study involved 170 tissues and cytological specimens. The cytological material included microcurettings, aspirates, brush preparations and tissue prints. An evaluation of the usefulness of the application of enzyme cytochemistry to cytological material is included. The most important results were a cyclic fluctuation and a progestagenic controlled increase in the activity of many oxidoreductases, especially the NADPH regenerating enzymes of the pentose phosphate pathway, and of the NADP+ dependent isocitrate dehydrogenase. The histochemical evaluation of the activity of these NADP+ linked enzymes can therefore be recommended for the evaluation of the physiological status of the endometrial cells, especially in patients with infertility problems.

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The histochemical demonstration of fructose diphosphate aldolase activity using a semipermeable membrane technique

Meijer

1985

Histochem J

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Semipermeable Membrane Techniques in Quantitative Enzyme Histochemistry

Cited by 12 publications

References 39 publications

Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy

Influence of shortwave laser therapy on morphometric parameters of sciatic nerve regeneration after experimental neurorrhaphy

Fluctuations in the enzymatic activity of the human endometrium

The histochemical demonstration of fructose diphosphate aldolase activity using a semipermeable membrane technique

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