Über das Actinomycin C (Antibiotica aus Actinomyceten, V. Mitteil.

Abstract

Aus einer bisher unbekannten Streptomyces‐Art, die als Str. chrysomallus bezeichnet ist, wurde Actinomycin C, ein rotes Antibioticum, isoliert, das Unterschiede gegenüber Actinomycin A und B aufweist. Durch energische Säurehydrolyse lassen sich aus Actinomycin C sechs Aminosäuren abspalten, die als l‐Threonin, d‐Valin, Sarkosin, d‐Allo‐isoleucin, l‐Prolin und N‐Methyl‐l‐valin identifiziert wurden. Bei der Hydrolyse mit Bariumhydroxyd entsteht eine rote, kristallisierte Verbindung, die dem chromophoren Teil des Actinomycins C entstammt. Die Actinomycine sind eine neuartige Gruppe von Naturstoffen, in denen eine chromophore Komponente wahrscheinlich peptidartig mit verschiedenen Aminosäuren verbunden ist.

The N-terminal and sulphur-containing residues of bacitracin A

“…They were therefore consistent with the assumption that an isoleucine residue occupies the N-terminal position in the intact molecule of bacitracin A, and that the alloisoleucine in hydrolysates is derived from this residue. It may be mentioned that Brockmann, Grubhofer, Kass & Kalbe (1951) formation of 1-amino-2-methyl-n-butane and of ethylamine involves the breaking of carboncarbon bonds. This is known to occur, to a minor degree, during the action of Raney nickel on penicillin (Kaczka & Folkers, 1949).…”

Extraction

“…The most striking contribution of countercurrent distribution during the past year has probably been made in the polypeptide field. It has been used by Brink et al in purifying subtilin (12), by Brockmann et al in purifying polypeptide antibiotics from Actinomycetes (13)(14)(15), by Payne et al in purification of ACTH (67), by du Vigneaud and collaborators on vasopressin (87) and oxytocin (63), by Felix et al on clupein (33), by Velick and Udenfriend in their isotope dilution method (88) for amino acid analysis, and by Kenner (54) in purifying synthetic peptide derivatives. Where isolation of amino acids from hydrolyzates is desired, it is a useful tool (25,27,33).…”

Biosynthesis, Structure, and Biological Action of the Lipids of the Tubercle Bacillus

“…The name peptidolipin NA was proposed; the substance(m.p. 232-233 "C; = +42 O ; C47H83N7012) contains a fatty acid which is bound by an amide link to a heptapeptide ~-Thr2, L-Alal, D-Atal, L-Val] , D-allo-lleul , L-ProI .D-allo-Isoleucine, found in peptidolipin NA by Ikawa and Snell[42], has u p till now only been isolated from actinomycin C3 by Brockmann et al[43].…”