Tyrosinase biosensor based on multiwall carbon nanotubes – titanium oxide nanocomposite for catechol determination

Fathy, Shadia A.; Hamid, Fatma F. Abdel; Nemr, Ahmed El; El‐Maghraby, Azza; Serag, Eman

doi:10.5004/dwt.2018.22847

Cited by 10 publications

(3 citation statements)

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“…The use of electroanalytical techniques in combination with enzymes ensures adequate selectivity for the determination of polyphenols, such as CA. In this context, enzymatic biosensors have been extensively studied to determine polyphenols in beverages or foods [157][158][159]. Tyrosinase and laccase have been frequently used due to their acting on the same types of compounds, for example monophenols and o-diphenols, which are related to the antioxidant capacity of beverages [160][161][162].…”

Section: Biosensors Based On Conductive Polymers and Carbon Nanomater...mentioning

confidence: 99%

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

Bounegru

Apetrei

2020

IJMS

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Caffeic acid is one of the most important hydroxycinnamic acids found in various foods and plant products. It has multiple beneficial effects in the human body such as antioxidant, antibacterial, anti-inflammatory, and antineoplastic. Since overdoses of caffeic acid may have negative effects, the quality and quantity of this acid in foods, pharmaceuticals, food supplements, etc., needs to be accurately determined. The present paper analyzes the most representative scientific papers published mostly in the last 10 years which describe the development and characterization of voltamperometric sensors or biosensors based on carbon nanomaterials and/or enzyme commonly used for detecting caffeic acid and a series of methods which may improve the performance characteristics of such sensors.

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Section: Biosensors Based On Conductive Polymers and Carbon Nanomater...mentioning

confidence: 99%

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

Bounegru

Apetrei

2020

IJMS

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“…Surface properties, including surface chemistry, surface roughness, surface energy and morphology are key factors that severely influence the structure of adsorbed enzymes. To obtain excellent performance, a wide range of materials were applied to fabricate TYR based biosensors, such as metal‐organic framework, [24] carbon nanotube, [25] graphene oxide, [26] conducting polymer, [27] 2D nanomaterials, [28,29] metal nanoparticles [30] and biomass [31] . However, it is rare to use sulfide minerals (SFMs) as the substrate materials for electrochemical sensors.…”

Section: Introductionmentioning

confidence: 99%

Effect of Acridine Orange on Improving the Electrochemical Performance of Tyrosinase Adsorbed Sulfide Minerals Based Catechol Biosensor

et al. 2023

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A highly sensitive and selective electrochemical biosensor for catechol (CC) detection was developed by immobilizing tyrosinase (TYR) on a glassy carbon electrode (GCE) modified with sulfide minerals (SFMs) in the co‐adsorption of acridine orange (AO). The sensor employed natural SFMs as the substrate material and utilized AO as a molecular recognition probe to create synergy effect for TYR to detect catechol. The developed TYR/AO/SFMs/GCE biosensors were characterized by scanning electron microscope, energy dispersive analysis of X‐rays spectroscopy, X‐ray diffraction spectra, X‐ray fluorescence, and electrochemical analysis. The results show that the sensors have excellent electrochemical activity for the detection of catechol (CC) under optimized conditions. The linear ranges were 0.1–50 μM, 0.1–10 μM, 0.1–50 μM and 0.1–100 μM for galena, pyrite, chalcopyrite and sphalerite, respectively, with the detection limits of 1.18 μM, 0.29 μM, 0.54 μM and 0.68 μM, respectively. The sensors also have good reproducibility, stability, repeatability and anti‐interference ability. AO is essential to increase the adsorbed amount and provides suitable orientation of the adsorbed TYR on these sulfide minerals’ surfaces. In consideration of cost‐effective and rich resources of sulfide minerals, easily physical adsorption method, combination of organic dye, minerals and enzymes, this strategy paves a new way to design other novel electrochemical biosensors.

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“…Moreover, the combined use of enzymes with electroanalytical techniques provides adequate selectivity for the polyphenol index determination. In this context, enzymatic biosensors have been widely studied to determine polyphenols in beverages [20,21,22,23,24,25,26,27]. Tyrosinase and laccase enzymes have been frequently used due to their associated substrates, o-diphenols and monophenols, which have been previously related to the antioxidant capacity of beverages [7,9].…”

Section: Introductionmentioning

confidence: 99%

Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method

García-Guzmán¹,

López-Iglesias²,

Aguilera³

et al. 2018

Sensors

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The application of a novel Poly(3,4-ethylenedioxythiophene)-Tyrosinase/Sonogel-Carbon electrode (PEDOT-Tyr/SNGC) biosensor to beers and wines analysis is proposed. This biosensor implies a new Sinusoidal Current (SC) electrodeposition method to immobilize the enzyme generating a nanostructure surface. The biosensors were characterized electrochemically, employing cyclic voltammetry and electrochemical impedance spectroscopy. Sensitivity, limit of detection, and correlation coefficients of the linear fitting were 2.40 × 10−4 µA·µM−1, 4.33 µM, and R2 = 0.9987, respectively. Caffeic acid is used as the reference polyphenol. A sampling of nine beers (four lager, three stout, and two non-alcoholic beers), and four wines (three red and one white wine) purchased in a local store was performed. The Polyphenol indeces for beers and wines have been assessed using the proposed biosensor, and the obtained values are in agreement with the literature data. Antioxidant properties of the samples using the 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid (ABTS) radical spectrophotometric method were also evaluated. The correlation between the polyphenol index and the antioxidant capacity was obtained for beers and wines.

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Tyrosinase biosensor based on multiwall carbon nanotubes – titanium oxide nanocomposite for catechol determination

Cited by 10 publications

References 0 publications

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

Voltamperometric Sensors and Biosensors Based on Carbon Nanomaterials Used for Detecting Caffeic Acid—A Review

Effect of Acridine Orange on Improving the Electrochemical Performance of Tyrosinase Adsorbed Sulfide Minerals Based Catechol Biosensor

Assessment of the Polyphenol Indices and Antioxidant Capacity for Beers and Wines Using a Tyrosinase-Based Biosensor Prepared by Sinusoidal Current Method

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