Typing ofPseudomonas aeruginosa: comparison of the phage procedure with the pyocine technique

Abstract

Two hundred and sixty strains of Pseudomonas aeruginosa were isolated from patients of the Asaf Harofe Government Hospital. The strains were typed by phage technique and 64 of them were also typed according to pyocine sensitivity. The two methods proved complementary, and reduced the number of untypable strains. Phage typing was performed with both routine test dilution (RTD) and more concentrated phage suspensions. The most reliable results were obtained at 100 RTD.

Comparative Genomic Analysis of 130 Bacteriophages Infecting Bacteria in the Genus Pseudomonas

“…Pseudomonas phages play key roles in shaping the diversity and evolution of bacteria through many mechanisms ( Paulsen et al, 2005 ), including horizontal gene transfers and alteration of host virulence ( Hayashi et al, 1990 ; Sano et al, 2004 ). Research of phages has been applied broadly in epidemiological studies of Pseudomonas diseases over the last 50 years, including the identification and sub-classification of pathogenic strains ( Bernstein-Ziv et al, 1973 ; Lindberg and Latta, 1974 ; Bergan, 1978 ), DNA transduction to introduce genes to bacterial cells ( Darzins and Casadaban, 1989 ; Rolain et al, 2011 ), and therapeutic strategy for antibiotic-resistant Pseudomonas infections ( Hraiech et al, 2015 ; Pires et al, 2015 ; Yayan et al, 2015 ). Therefore, improved understanding of Pseudomonas phages should contribute to a broader understanding of Pseudomonas bacteria and to the development of molecular tools and clinical applications.…”