Chapter V Phage Typing of Pseudomonas aeruginosa

Bergan, Tom

doi:10.1016/s0580-9517(08)70662-7

Cited by 16 publications

(10 citation statements)

References 52 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Variation in the phage susceptibility of strains may be due to the ratio of infectious phage particles to bacterial cells, temperature of incubation, media and the recording of lyric reactions. When the same pseudomonad strains were retyped on different days in Bergan's study (60), only 60% showed identical pattern reactions. If one major reaction difference was allowed, then about 11% of strains would be considered different on retyping.…”

Section: Interpretation Of Phage Typingmentioning

confidence: 97%

Epidemiological typing ofPseudomonas aeruginosa

1988

Eur. J. Clin. Microbiol. Infect. Dis.

100

View full text Add to dashboard Cite

Current knowledge of the typing of Pseudomonas aeruginosa and new methods for characterizing strains are reviewed. A combination of serotyping as a primary screen with pyocin typing for finer discrimination between isolates gives valid epidemiological data, is within the scope of most clinical laboratories and is to be recommended. Phage typing and H typing do provide good discrimination but are not reproducible in practice because a reaction-difference rule has to be applied to phage patterns, and diphasic variation reduces the accuracy of the identification of flagellar antigens. A problem remains with cystic fibrosis isolates, which show considerable heterogeneity in surface properties. For these strains pyocin production typing by the revised method of Govan is indicated. Newer techniques such as isoenzyme profile and DNA probes of the chromosome require independent evaluation and due to their technical difficulty may not be adopted in a routine context for some years to come.

show abstract

Section: Interpretation Of Phage Typingmentioning

confidence: 97%

Epidemiological typing ofPseudomonas aeruginosa

1988

Eur. J. Clin. Microbiol. Infect. Dis.

100

View full text Add to dashboard Cite

show abstract

“…The typing phages used were obtained from the Central Public Health Laboratory, London, UK. The typing set consists of 20 phages: 7, 16, 21, 24, 31, 44, 68, 73, F7, F8, F10, 109, 119x, 352, 1214, M4, M6, Cll, C18 and C21 (4). Petri dishes of agax were prepared containing 2.5 g Oxoid NB, 0.8 g Difco-Bacto-agar and 100 ml distilled water at pH 7.4; after sterilisation 4 ml 0.1M CaC12 per 100 ml medium were added.…”

Section: Bacteriamentioning

confidence: 99%

Marked increase ofPseudomonas aeruginosa serotype 012 in Belgium since 1982

Allemeersch

Beumer

Devleeschouwer

et al. 1988

Eur. J. Clin. Microbiol. Infect. Dis.

View full text Add to dashboard Cite

Routine typing was performed on a total of 7089 Pseudomonas aeruginosa strains isolated in 16 Belgian hospitals in the period from 1977 to 1986. The annual number of strains received ranged from 318 to 1346. The incidence of serotype O:12 was less than 2% until 1981 when it rose to 4%, steadily increasing to become the predominant serotype in 1984 (22%), 1985 (18%) and 1986 (22%). Since 1980 the O:12 isolates have exhibited characteristic patterns on pyocin and phage typing, 89% of O:12 isolates belonging to pyocin types 1, 39, 43, 45 or 105, whereas only 51% of isolates of other serotypes belonged to those pyocin types. Ninety-three per cent of serotype O:12 isolates belonged to phage types 68/119x, 68 or 119x, or were non-typable, whereas only 24.37% of other serotypes isolates exhibited these phage patterns. These distinctive patterns of pyocin and phage types suggest a high degree of homogeneity within the O:12 strains isolated in recent years in Belgium. Multi-centre or country-wide survey of Pseudomonas aeruginosa strains isolated in hospitals using epidemiological markers may be of value in identifying a sudden increase in epidemic strains.

show abstract

“…Lack of consistent results and the elaborate work required to maintain typing phage preparations have made phage typing only moderately useful for P . aeruginosa (Bergan, 1978) ; poor reproducibility has been of some, although lesser, concern in the case of pyocin typing (Fyfe et al, 1984). Serotyping gives better reproducibility (Linyi and Bergan, 1978) but is unsatisfactory for mucoid strains of P .…”

Section: Introductionmentioning

confidence: 99%

Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa

Poh

Yap

Tay

et al. 1988

Journal of Medical Microbiology

View full text Add to dashboard Cite

Summary. The plasmid profiles of 1 12 clinical isolates of Pseudomonas aeruginosa were determined by two reproducible, rapid, plasmid screening methods. Plasmid DNA was present in 15% of isolates examined. Plasmids varied in size from 1.2 x lo6 to 60.2 x lo6 mol. wt. The dominant serotypes encountered were 0 : 11 and 0:4, which comprised 38% and 12% of strains, respectively. Four pyocin types (1, 10, 3 and 5) dominated (respective frequencies: 56, 15, 12 and 6%). Reproducibility of pyocin typing was distinctly inferior to both plasmid profiling and serotyping. Strains of identical serotypes could be further differentiated by dissimilar plasmid profiles. Serologically untypable or polyagglutinable strains were successfully characterised by plasmid profile patterns. Thus, plasmid profiling was shown to be a useful adjunct to serotyping for the epidemiological typing of P . aeruginosa.

show abstract

Chapter V Phage Typing of Pseudomonas aeruginosa

Cited by 16 publications

References 52 publications

Epidemiological typing ofPseudomonas aeruginosa

Epidemiological typing ofPseudomonas aeruginosa

Marked increase ofPseudomonas aeruginosa serotype 012 in Belgium since 1982

Plasmid profiles compared with serotyping and pyocin typing for epidemiological surveillance of Pseudomonas aeruginosa

Contact Info

Product

Resources

About