Typing of Clostridium perfringens isolated from some meat products by using PCR

Tawab, Ashraf Abd Al; Khater, Dalia F.; Kotb, M

doi:10.21608/bvmj.2015.31803

Cited by 3 publications

(2 citation statements)

References 8 publications

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“…Further biochemical tests were confirmative to C. perfringens; where isolates were positive for glucose, lactose and sucrose sugar fermentation; liquefied gelatin while they were negative for catalase and indole tests. The findings may be consistent with parallel studies carried out by Cowan et al (2004); ISO (2004); Abd Al-Tawab et al (2015).…”

Section: Discussionsupporting

confidence: 93%

Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market

Tawab

Abdallah

Yusuf

2017

Benha Veterinary Medical Journal

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The present study was designed for isolation and identification of Clostridium perfringens (C. perfringens) from herbs and spices that are widely distributed in the Egyptian markets; and determination of the drug of choice for its control based on the Antimicrobial Susceptibility Test (AST). A total of 392 samples of herbs and spices were randomly and anonymously selected from the enormous number of samples obtained from the Egyptian markets. An overall occurrence of C. perfringens was 8.42% (33 out of 392). Separately, occurrence was higher in herbs than spices being 10.5 and 6.25%, respectively. C. perfringens isolates were identified and confirmed with macroscopical, microscopical and biochemical analysis. AST results revealed that isolates of C. perfringens from the tested herbs and spices samples were found most susceptible to ampicillin-sulbactam and Penicillin-G with inhibition zones of 19.4 ± 0.98 and 16.6 ± 1.16 mm, respectively. On the other hand, the isolates were found nearly resistant to clindamycin and vancomycin with inhibition zones of 6.28 ± 0.63 and 8.78 ± 0.41 mm, respectively. Meanwhile, the isolates were intermediary susceptible to trimethoprimsulphamethoxazole combination and chloramphenicol. Data of the present study indicate occurrence of C. perfringens in herbs and spices with a considerable rate and the antimicrobials of choice for its control are penicillins and ceftazidime. higher sanitation procedures in herbs and spices industry before its distribution to the Egyptian markets have been recommended.

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Section: Discussionsupporting

confidence: 93%

Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market

Tawab

Abdallah

Yusuf

2017

Benha Veterinary Medical Journal

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“…According to multiplex PCR results as showing in table (2) and pic.1, out of examined C. perfringens isolates, 29.6% of isolates were confirmed as C. perfringens type A (cpa), and 3.7% isolate was C. perfringens type D (etx); while failed to detect enterotoxin production gene (cpe); which were in agree with (Erol et al, 2008) who reported that recent studies claimed that C. perfringens type A foodborne poisonings are rarely associated with enterotoxin encoding by the cpe gene it is only detected in (0-5%) of outbreaks isolates. So, our results are logically in agree with (Abd Al-Tawab et al, 2015) (Prabhu et al, 2013, (Salah El-din et al, 2015) who proved that all their PCR examined C. perfringens isolates gave a characteristic band at 402bp (cpa only); Torky and Hassan (2014) revealed 66% of multiplex PCR examined isolates gave bands at 402bp and 33.3% gave both bands at 402 and 541bp, which referring to C. perfringens type A (cpa) and C. perfringens type D (etx), respectively; while failed to detect either C. perfringens type B or C. While it disagreed with (Afshari et al, 2015) who detected type A (29.03%), type C (70.96%), and cpe (25.00%) toxin genes in examined C. perfringens isolates.…”

Section: Discussionsupporting

confidence: 89%

Detection and typing of Clostridium perfringens in some retail chicken meat products

Shaltout

Zakaria

2017

Benha Veterinary Medical Journal

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Clostridium perfringens is considered as one of major food poisoning bacteria; which may refer to different lethal toxins production including C. perfringens enterotoxin. C. perfringens toxins have been contributed in many diseases in human being especially C. perfringens type A enterotoxin food poisoning. A total of 125 random raw and half cooked chicken meat samples represented by (breast, thigh, nuggets, panée and frankfurter "25 of each") were collected from various retail stores and supermarkets in Qualyubia governorate to investigate the presence of C. perfringens bacteriologically and detect the cpa, etx, and cpe toxin genes by multiplex PCR. Results demonstrated that 6 out of 25 raw breast samples (24%), 8 out of 25 raw thigh samples (32%), 5 out of 25 nuggets samples (20%), 4 out of 25 panée samples (16%), and 4 out of 25 frankfurter samples (16%) were found to be contaminated with C. perfringens. Twenty-seven positive isolates obtained from these samples were identified as C. perfringens based on the microscopic examination and biochemical tests. It was detected that 8 (29.6%) out of 27 C. perfringens isolates carried only alpha toxin gene (type A), and only one isolate (3.7%) of them expressed both alpha and epsilon toxin genes (type D); while cpe gene never had been detected in any examined isolate, according to the multiplex PCR results.

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Emergency of extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa isolated from broiler chickens in Egypt

Ahmed,

Nasef,

El Enbaawy

2024

BJVM

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Multidrug-resistant (MDR) bacteria, such as extended-spectrum beta-lactamase (ESBL) Enterobacteriaceae, pose a challenge to human and animal health care systems. Antibiotic resistance in non-lactose fermenting pathogens such as Pseudomonas aeruginosa is increasing, making these clinical pathogens more difficult to treat. The most predominant Pseudomonas species that cause mortality among birds, especially chickens, is P. aeruginosa. The present study detected multi-drug resistance and ESBL-production among P. aeruginosa isolated from broiler chickens in different poultry farms. A total of 450 broiler chickens from 150 private farms were examined for the presence of P. aeruginosa using bacteriological and molecular methods. Samples were collected from the internal organs of the birds and subjected to bacteriological examination and identification. The bacteriological examination revealed that 29 farms (19.33%) were positive for P. aeruginosa. On the other hand, by PCR, only 25 farms (16.66%) were positive. All P. aeruginosa isolates were subjected to in vitro antimicrobial susceptibility testing against 15 antimicrobial agents by the disc diffusion method. P. aeruginosa isolates showed resistance to a wide range of antimicrobial agents, including trimethoprim/sulfamethoxazole (80%), followed by streptomycin (56%), colistin sulfate (48%), ceftazidime (32%), gentamicin and ciprofloxacin (28% each). In contrast, resistance levels to cefotaxime, oxytetracycline, and norfloxacin were 24%, 20%, and 16%, respectively. The lowest resistance was against apramycin (12%), fosfomycin, imipenem, and ertapenem (8%). All P. aeruginosa isolates were sensitive to amikacin and meropenem. The antibiotic-resistant pattern of the isolated P. aeruginosa revealed that the multi-drug resistance (MDR) level was 14/25 (56%). Only 16 isolates (64%) were ESBL producing. From the ESBL isolates, 9/16 (56.25%) were MDR. Molecular detection for antibiotic resistance gene revealed that blaTEM was detected in 20/25 (80%) of P. aeruginosa isolates. From the blaTEM gene-positive P. aeruginosa, 16/20 isolates (80%) were ESBL producers, and 11/20 (55%) were MDR. The results indicated that MDR and ESBL producing P. aeruginosa frequency reached an alarming level in poultry isolates in Egypt. In conclusion, the misuse of antibiotics leads to the development of resistant bacteria that may transfer from poultry to humans. Strict supervision and enforcement of laws to control antibiotic usage in the food chain within established safe levels must be done.

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Typing of Clostridium perfringens isolated from some meat products by using PCR

Cited by 3 publications

References 8 publications

Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market

Incidence and antibiogram of Clostridium perfringens isolated from herbs and spices widely distributed in the Egyptian market

Detection and typing of Clostridium perfringens in some retail chicken meat products

Emergency of extended-spectrum beta-lactamase-producing Pseudomonas aeruginosa isolated from broiler chickens in Egypt

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