IntroductionContagious equine metritis (CEM) was first described in the United Kingdom (UK) in 1977, and then become world-wide [1].Contagious equine metritis is an inflammatory disease of the proximal and distal reproductive tract of the mare caused by Taylorellaequigenitalis, causing mares temporary infertile [2].Taylorellaequigenitalis is a Gram-negative, microaerophiliccocco bacillus bacterium, transmitted during mating.Infected mares had a profuse, mucopurulent vaginal discharge up to 40% of affected mares due to inflammation C ONTAGIOUS Equine Metritis (CEM) is a transmissible venereal disease of equine, causes infertility in mares and spontaneous abortion in pregnant ones. Theetiological agent of CEM is Gram negative bacteria; Taylorellaequigenitalis. In this study,a number of45 clitoral swabswere collected from suspected mares and twenty swabs were fromurethral fossa of stallions. Swabs were kept cool during transportation on Amies with charcoal medium. At the lab, all swabs during 48 hours since they have been collected then cultured on tryptose chocolate blood agar (TBA) plates, and incubated at 37ºC in microaerophilic atmosphere of 5%-10% CO 2 in hydrogen of 7 days. The suspected colonies have been examined for biochemical characters of catalase, oxidase and phosphatase.Allmareswere scannedby ultrasound scanner (Sono Scape sonar), vaginal and/or rectal, checked for the presence of any uterine fluid which may indicate the presence of infection. On the other hand, culture investigations revealed 2 (10%) positive cases among stallions and 5 (11.1%) mare's culture positive for T. equigenitalis. The bacterium is catalase, oxidase and phosphatase positive. The colonies were confirmed by immunofluorescent test and its sensitivity was 100%.Ultrasound examination of three mare's uteri showing pyometra where their lumen measured 40.6-57.3 mm fully filled with echogenic particles. While, the uteri of the other two mares showingendometritis which appearedas an echogenicuterine lumen measured 18.6 mm and 37.6 mm with echogenic particles scattered on it. Biosecurity practices can help in preventingspread of CEM by bacterial culture tests on breeding stallions prior to breeding season. Also, urethral swab for CEM testing should be a stallion's annual breeding exam. According to this study an ultrasound scan at the breeding season to check for the soundness of the mare uteri is strongly recommended.
One hundred and twenty random samples of meat products from different supermarkets in El-Gharbia governorate including minced meat, beef burger, sausage, luncheon (30 samples of each) were collected. C. perfringenswas detected in meat products with an incidence 15%. The incidence of C.perfringens in minced meat, sausage, beefburger and luncheon was 16.67%, 23.33%, 16.67% and 3.33%, respectively. Typing of C.perfringens isolates revealed that the incidences of toxigenic and non-toxigenic strains were 83.33% and 16.67%, respectively. Typing of toxigenic strains of C.perfringens revealed that all isolates were type (A). Polymerase chain reaction (PCR) was applied for detection of toxin genes and enterotoxin of C.perfringens. All tested isolates were positive for alpha toxin gene with amplified PCR product of 402 bp and were cpe negative. Public health importance of C.perfringens isolates was discussed.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.
hi@scite.ai
10624 S. Eastern Ave., Ste. A-614
Henderson, NV 89052, USA
Copyright © 2024 scite LLC. All rights reserved.
Made with 💙 for researchers
Part of the Research Solutions Family.