Type I collagen in xenogenic bone biomaterial regulates attachment and spreading of osteoblast via the β1 integrin subunit

Basle, Michel‐Félix; Lesourd, M.; Grizon, F.; Pascaretti, C.; Chappard, Daniel

doi:10.1007/pl00003479

Cited by 15 publications

(12 citation statements)

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“…In particular, collagen has motifs to facilitate binding of the ␤ 1 integrin subunit of osteoblasts. 37 Nevertheless, when IGF-I was prebound to the VN-coated collagen sponges or membrane of Transwells, cell migration was significantly enhanced. This stimulation of cell migration was further strengthened by the additional binding of IGF-I and IGFBP-5 to VN.…”

Section: Discussionmentioning

confidence: 98%

Surface Modification by Complexes of Vitronectin and Growth Factors for Serum-Free Culture of Human Osteoblasts

et al. 2005

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Cell attachment, expansion, and migration in three-dimensional biomaterials are crucial steps for effective delivery of osteogenic cells into bone defects. Complexes composed of vitronectin (VN), insulin-like growth factors (IGFs), and insulin growth factor-binding proteins (IGFBPs) have been reported to enhance cell attachment, proliferation, and migration in a variety of cell lines in vitro. The aim of this study was to examine whether prebound complexes of VN and IGFs +/- IGFBPs could facilitate human osteoblast serum-free expansion in vitro and enhance cell attachment, proliferation, and migration in three-dimensional biomaterial constructs. Human osteoblasts derived from alveolar bone chips and the established human osteoblast cell line Saos-2 were used. These cells were seeded on tissue culture plates and porous scaffolds of type I collagen sponges and polyglycolic acid (PGA), which had been coated with VN +/- IGFBP-5 +/- IGF-I. Cell attachment, proliferation, and migration were evaluated by cell counting, confocal microscopy, and scanning electron microscopy. The number of attached human osteoblasts was significantly higher in VN-coated polystyrene culture dishes. Furthermore, significant increases in cell proliferation were observed when growth factors were bound to these surfaces in the presence of VN. In the two scaffold materials examined, greater cell attachment was found in type I collagen sponges compared with PGA scaffolds. However, coating the scaffolds with complexes composed of VN + IGF-I or VN + IGFBP-5 + IGF-I enhanced cell attachment on PGA. Moreover, the presence of VN + IGFBP-5 + IGF-I resulted in significantly greater osteoblast migration into deep pore areas as compared with untreated scaffolds or scaffolds treated with fetal calf serum. These results demonstrated that complexes of VN + IGFBP-5 + IGF-I can be used to expand osteoblasts in vitro under serum-free conditions and enhance the attachment and migration of human osteoblasts in three-dimensional culture. This in turn suggests a potential application in surface modification of biomaterials for tissue reconstruction.

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Section: Discussionmentioning

confidence: 98%

Surface Modification by Complexes of Vitronectin and Growth Factors for Serum-Free Culture of Human Osteoblasts

et al. 2005

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“…Obviously, cells do not adhere directly to metallic implant surfaces but through an intermediate, conditioning layer composed of proteins and glycoproteins. 36,37 Contrarily, Gronowicz and McCarthy 1 suggest that cells are able to bind directly to metallic implant surfaces. 31 Although no differences in the percentage of adherent cells were observed, SEM show ultrastructural differences in cell spreading and filopodia forming in dependence on the given surface.…”

Section: Discussionmentioning

confidence: 99%

Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts

Hempel

Wolf

et al. 2000

J. Biomed. Mater. Res.

223

144

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The initial contact of osteoblasts with implant surfaces is an important event for osseointegration of implants. Osseointegration of Ti6Al4V may be improved by precoating of its surface with collagen type I. In this study, the adhesion of rat calvarial osteoblasts to uncoated and collagen type I-coated titanium alloy was investigated over a period of 24 h. Collagen type I-coating accelerates initial adhesion of osteoblasts in the presence of fetal calf serum. One hour after plating, no differences in the percentage of adherent cells between the surfaces investigated were found. Adhesion of osteoblasts to uncoated surfaces was reduced by the GRGDSP peptide by about 70%, whereas adhesion to collagen type I-coated surfaces remained unaffected by treatment of the cells with the peptide. Cell adhesion to coated materials was reduced by about 80% by anti-integrin beta1 antibody. The integrin beta1 antibody did not influence the adhesion to uncoated titanium alloy. The results suggest that osteoblasts adhere to collagen type I-coated materials via integrin beta1 but not by interacting with RGD peptides, whereas adhesion to uncoated titanium alloy is mediated by RGD sequences but not via integrin beta1. Fibronectin does not seem to be involved in the adhesion of osteoblasts to either coated or uncoated titanium alloy.

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“…That process preserves bone collagen in its native state, which should allow for an improved bone regeneration process, given collagen's well-known biological properties. [24][25][26][27][28][29][30][31] Indeed, osteoclasts cultured over such equine, enzymatically deantigenated, and collagenpreserving bone substitutes have significantly higher adhesion and activity than that found for osteoclasts grown over collagen-free, totally deproteinized bovine bone. 32,33 When sites augmented with equine bone alone were compared with others augmented with the same material added to autogenous bone, immunohistochemical tests showed no differences between the two regarding the expression of some biochemical markers of bone regeneration.…”

Section: 16mentioning

confidence: 99%

Preserving the Bone Profile in Anterior Maxilla using an Equine Cortical Bone Membrane and an Equine Enzyme-treated Bone Graft: A Case Report with 5-year Follow-up

Stefano

Garagiola

Bassi

2017

The Journal of Contemporary Dental Practice

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Aim: This study aimed to report a well-succeeded use of equine enzyme-deantigenic cortical membrane and bone granules for guided bone regeneration (GBR) in the esthetic zone concomitant with implant placement. Background:In the anterior maxilla, where patients typically have the highest expectations for implant-supported restorations, bone resorption in the wake of tooth loss often leads to a lack of soft tissue support and ultimately to unsatisfactory esthetic results. Buccal bone augmentation at the time of implant placement has thus become common. This is usually accomplished following GBR principles and employing membranes made from various materials to serve as a barrier between the soft tissue and graft material. One of the more recently introduced membrane materials is made from thin, flexible equine cortical bone, i.e., rendered nonantigenic in an enzymatic process that preserves native bone collagen.Case report: This report describes the treatment of a patient who received an equine enzyme-deantigenic graft and membrane in conjunction with placement of an implant in the right maxillary lateral incisor site. Conclusion:After 5 years of follow-up, the patient was very satisfied with her appearance. A cone-beam computed tomography scan showed that the peri-implant bone levels and ridge thickness had been maintained, and the cortical layer in the pristine ridge had also undergone remodeling. Clinical significance: Guided bone regeneration with the concomitant use of enzyme-deantigenic membrane and graft is a valuable and suitable option for effective implant-supported prosthetic rehabilitation in the esthetic zone. Preserving the Bone Profile in Anterior Maxilla

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Type I collagen in xenogenic bone biomaterial regulates attachment and spreading of osteoblast via the β1 integrin subunit

Cited by 15 publications

References 0 publications

Surface Modification by Complexes of Vitronectin and Growth Factors for Serum-Free Culture of Human Osteoblasts

Surface Modification by Complexes of Vitronectin and Growth Factors for Serum-Free Culture of Human Osteoblasts

Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts

Preserving the Bone Profile in Anterior Maxilla using an Equine Cortical Bone Membrane and an Equine Enzyme-treated Bone Graft: A Case Report with 5-year Follow-up

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