Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts

U, Geissler; Hempel, Ute; Wolf, Christine; Scharnweber, Dieter; Worch, H.; Wenzel, Klaus-Wolfgang

doi:10.1002/1097-4636(20000915)51:4<752::aid-jbm25>3.0.co;2-7

Cited by 223 publications

(158 citation statements)

References 39 publications

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“…Coating titanium surface with collagen enhanced osteoblast adhesion and proliferation [20,21]. An in vitro study using bone marrow cells also showed that surface coating with type I collagen showed high ALP activity, collagen synthesis, and mineralized matrix formation [22].…”

Section: Collagensupporting

confidence: 51%

Research Progress on the Biochemical Modification of Titanium Implant Surface

Mao¹

2014

Med chem

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Section: Collagensupporting

confidence: 51%

Research Progress on the Biochemical Modification of Titanium Implant Surface

Mao¹

2014

Med chem

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“…It may also be possible that the metal ion release from the samples affected the initial production of FN. A previous study has demonstrated that FN was not involved in the adhesion of osteoblasts to uncoated Ti alloy at 24 h [25]. Moreover, the integrin a 5 Fwhich is a major adhesion receptor of osteoblasts interacting with FN [26,27]Fwas not detected in cell cultured on polished or rough Ti-6A1-4V at 12 h while it was detected for cell cultured on polystyrene [28].…”

Section: Discussionmentioning

confidence: 84%

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Ku¹,

et al. 2002

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The purpose of the present work was to examine the effect of different Ti-6Al-4V surface treatments on osteoblasts behaviour. Previous work in this laboratory has demonstrated that an ageing treatment reduces metal ion release from this alloy compared to standard passivation procedures. In this study, human osteosarcoma MG-63 were used in short-term in vitro tests to assay for cell viability and cell proliferation at 12, 24 and 72 h while SaOS-2 were used in long-term in vitro tests to assay for osteonectin, osteopontin, osteocalcin gene expression, total protein amount (TP), alkaline phosphatase activity (ALP) and fibronectin production (FN) for 1-4 weeks. Epifluorescence microscopy was used to observe SaOS-2 cell morphology. After 24 h, there was no difference in MG-63 cell viability/proliferation or in SaOS-2 cell morphology between the different surface treatments. For the longterm tests, the aged Ti-6Al-4V induced significantly higher cell proliferation than the control Ti-6Al-4V at 72 h. At week 1, no difference in the osteonectin, osteopontin, and osteocalcin gene expression was found between samples. The peak of ALP activity appeared earlier at week 2 for the control surface compared with the passivated and aged surfaces. The early increase in ALP activity for the control sample could be a compensatory effect of decreased osteoblasts proliferation. There was no difference in the expression of FN for the different surface treatments. Our present results showed that the different surface treatments, which induced different metal ion release kinetics and surface properties, influenced the cell proliferation and ALP activity of osteoblast cells. Aluminium ions release kinetics as well as presence of vanadium ions may play a major role in influencing the osteoblasts behaviour in the present study. r

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“…35 While various bioactive peptides (RGD, COL1, fibronectin) have shown initial increases in cellular adhesion of cells attached to the modified Ti surface their effect on late differentiation has had mixed success. 22,[36][37][38][39][40][41][42][43] Our study shows that presence of P15 increases osteogenic gene expression and stimulates osteoblastic activity; as shown by qPCR and histochemical staining of cells grown on Ti-P15 surface compared to control.…”

Section: Discussionmentioning

confidence: 99%

Covalent attachment of P15 peptide to titanium surfaces enhances cell attachment, spreading, and osteogenic gene expression

Liu

Limthongkul

Sidhu

et al. 2012

Journal Orthopaedic Research

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P15, a synthetic 15 amino acid peptide, mimics the cell-binding domain within the alpha-1 chain of human collagen is being tested in clinical trials to determine if it enhances bone formation in spinal fusions. We hypothesize that covalent attachment of P15 to titanium implants may also serve to promote osseointegration. To test this hypothesis, we measured osteoblast and mesenchymal cell adhesion, proliferation, and maturation on P15 tethered to a titanium (Ti-P15) surface. P15 peptide was covalently bonded to titanium alloy surfaces and incubated with osteoblast like cells. Cell toxicity, adhesion, spreading, and differentiation was then evaluated. Real-time quantitative PCR, Western blot analysis, and fluorescent immunohistochemistry was performed to measure osteoblast gene expression and differentiation. There was no evidence of toxicity. Significant increases in early cell attachment, spreading, and proliferation were observed on the Ti-P15 surface. Increased filapodial attachments, a 2 integrin expression, and phosphorylated focal adhesion kinase immunostaining indicated activation of integrin signaling pathways. qRT-PCR analysis indicated there was significant increase in osteogenic differentiation markers in cells grown on Ti-P15 compared to control-Ti. Western blotting confirmed these findings. Surface modification of titanium with P15 significantly increased cell attachment, spreading, osteogenic gene expression, and differentiation. Results of this study suggest that Ti-P15 has the potential to safely enhance bone formation and promote osseointegration of titanium implants. ß

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Collagen type I-coating of Ti6Al4V promotes adhesion of osteoblasts

Cited by 223 publications

References 39 publications

Research Progress on the Biochemical Modification of Titanium Implant Surface

Research Progress on the Biochemical Modification of Titanium Implant Surface

Effect of different Ti–6Al–4V surface treatments on osteoblasts behaviour

Covalent attachment of P15 peptide to titanium surfaces enhances cell attachment, spreading, and osteogenic gene expression

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