Type 1 hypersensitivity reactions in reconstructed tissues using syngeneic cell types

Baird, Alan W.; Cuthbert, A. W.; MacVinish, Lesley J.

doi:10.1111/j.1476-5381.1987.tb11285.x

Cited by 25 publications

(8 citation statements)

References 27 publications

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“…Independent evidence that TTX can block the effects of direct electrical stimulation of the submucosal plexus has been given previously (Baird et al, 1987).…”

Section: Discussionmentioning

confidence: 82%

“…In an earlier report (Baird et al, 1987) we showed that in ileal preparations sensitised to I3LG the response to challenge (or electrical field stimulation) was inhibited by TTX. For the sake of completeness we have also carried out experiments with colon preparations from guinea-pigs that had been fed cows' milk.…”

Section: Methodsmentioning

confidence: 99%

“…In particular there is a large, inappropriate electrogenic secretion of chloride ions and an inhibition of electroneutral sodium chloride absorption, associated with fluid secretion towards the luminal side of the epithelium. We have also presented data (Baird et al, 1987) to show that tetrodotoxin (TTX) can inhibit hypersensitivity reactions of this type.…”

mentioning

confidence: 99%

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Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Baird¹,

Cuthbert²

1987

British J Pharmacology

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1 Using a number of 5-hydroxytryptamine (5-HT)-antagonists we have compared their activity against the chloride-secretory response in guinea-pig ileal and colonic epithelia when challenged with 5-HT or antigen. Guinea-pigs sensitized to P-lactoglobulin (PLG) were used throughout; these were obtained by providing animals with cows' milk for drinking. 2 Of methysergide, ketanserin, cyproheptadine and ICS 205-930, only the latter inhibited both the response to 5-HT and to antigen challenge. Methysergide caused a minor, significant effect on 5-HT but not on PLG responses. Ketanserin had no effect on the responses to 5-HT, but both ketanserin and cyproheptadine inhibited the challenge to PLG.3 The data are considered in relation to the current views of receptor subtypes for 5-HT. Some of the reported inhibitors may be non-specific, while we consider there is evidence to support the view that 5-HT3-receptors (neuronal receptors) are involved both in the responses to 5-HT and to antigen challenge. 4 Tetrodotoxin mimicked the effect of ICS 205-930 on both the response to 5-HT and to antigen challenge in sensitized tissues, confirming a neuronal involvement for both types of stimuli.

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“…Independent evidence that TTX can block the effects of direct electrical stimulation of the submucosal plexus has been given previously (Baird et al, 1987).…”

Section: Discussionmentioning

confidence: 82%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Baird¹,

Cuthbert²

1987

British J Pharmacology

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“…Recent morphological, pharmacological, and electrophysiological evidence indicates that interactions between enteric nerves and mast cells may importantly participate in some ofthese responses (1 1, 13-16). Finally, in vitro studies have demonstrated that chloride ion secretion is the force responsible for the changes in fluid and electrolyte transport observed during intestinal anaphylaxis (17)(18)(19) and that activated mast cells or mast cell lysates can induce secretion from intestinal epithelial cells maintained in tissue culture (20,21).…”

Section: Introductionmentioning

confidence: 99%

Role of mast cells in ion transport abnormalities associated with intestinal anaphylaxis. Correction of the diminished secretory response in genetically mast cell-deficient W/Wv mice by bone marrow transplantation.

Perdue¹,

Masson²,

Wershil³

et al. 1991

J. Clin. Invest.

151

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“…65 Mast cells are key components of immediate hypersensitivity events and other reactions to foreign antigens: their activation releases a plethora of mediators that effect a variety of physiological processes, including ion secretion and neuronal activity The ability of activated mast cells to alter directly epithelial ion transport was described in a seminal paper by Baird et d. 66 Filter-grown monolayers of human colonic HCA-7 epithelial cells were juxtaposed to filter pads containing 4 x lo5 peritoneal cells (-14% mast cells) from rats sensitized to ovalbumin. These cell sandwiches or 'reconstructed tissues' were mounted in Ussing chambers and ion transport was measured in response to ovalbumin challenge.…”

Section: Immune-mediated Epithelial Pathophysiologymentioning

confidence: 99%

Review article: In vitro models in inflammatory bowel disease research—a critical review

McKay

Philpott

Perdue

1997

Aliment Pharmacol Ther

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SUMMARY Research efforts in inflammatory bowel disease (IBD) have been directed towards the epithelium as it has become clear that epithelial cells play a critical role in inflammatory response. Most research involving IBD employs in vitro techniques. In vitro epithelial cell studies have played and are continuing to play a major role in providing specific information relevant to IBD. Thus, such studies have provided irrefutable evidence that epithelial responses can be induced by microbes/microbial products and by immune activation. Culture experiments have provided insights into the effects of individual cytokines and other inflammatory mediators on epithelial pathophysiology, injury and repair, apoptosis, necrosis, and other processes that may be involved in IBD. Activated epithelial cells can participate in and even orchestrate immune responses, by stimulating T cells (and possibly others) and by producing cytokines that recruit specific inflammatory cells. Physiological regulation of epithelial tight junctions has been demonstrated by in vitro studies; the implication of this information for treating IBD is just beginning to be explored. It is becoming increasingly clear that epithelial processing and presentation of antigens is critical to the outcome of the immune response.

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Type 1 hypersensitivity reactions in reconstructed tissues using syngeneic cell types

Cited by 25 publications

References 27 publications

Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Neuronal involvement in type 1 hypersensitivity reactions in gut epithelia

Role of mast cells in ion transport abnormalities associated with intestinal anaphylaxis. Correction of the diminished secretory response in genetically mast cell-deficient W/Wv mice by bone marrow transplantation.

Review article: In vitro models in inflammatory bowel disease research—a critical review

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