Two validated liquid chromatographic methods for the simultaneous determination of flumethasone pivalate, its related substance (flumethasone), and clioquinol

Sayed, Nour W.; Hegazy, Maha A.; Abdelaleem, Eglal A.; Abdelkawy, Mohamed; Abdelfatah, Rehab M.

doi:10.1556/jpc.27.2014.6.11

Cited by 8 publications

(14 citation statements)

References 7 publications

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“…Linearity range was 0.5-50 μg/mL [61]. Sayed et al [62] established an HPLC method for the simultaneous determination of flumethasone pivalate, flumethasone pivalate-related substance and impurity and clioquinol. Separation was achieved within 11 min using ODS column using acetonitrile-water (70:30 v/v) as the mobile phase at a flow rate of 1 mL/min with ultraviolet detection was achieved at 235 nm.…”

Section: -High-performance Liquid Chromatography Technique (Hplc)mentioning

confidence: 99%

“…Separation was achieved within 11 min using ODS column using acetonitrile-water (70:30 v/v) as the mobile phase at a flow rate of 1 mL/min with ultraviolet detection was achieved at 235 nm. The calibration plots were linear over the concentration range of 5-50, 2-35, and 10-70 mg/mL, respectively [62]. Chhalotiya et al [63] introduced a stability indicating RP-HPLC method for the determination of clioquinol in bulk and in pharmaceutical dosage forms.…”

Section: -High-performance Liquid Chromatography Technique (Hplc)mentioning

confidence: 99%

“…Sayed et al [62] developed a TLC-densitometric method for the simultaneous determination of flumethasone pivalate, flumethasone pivalate-related substance and impurity and clioquinol. The proposed TLC-densitometric was performed using silica gel plates 60 F254 as a stationary phase with benzene-hexane-acetone-formic acid (5:4:2:0.13, by volume) as a developing system followed by densitometric measurements at 235 nm.…”

Section: -Tlc Densitometrymentioning

confidence: 99%

“…The proposed TLC-densitometric was performed using silica gel plates 60 F254 as a stationary phase with benzene-hexane-acetone-formic acid (5:4:2:0.13, by volume) as a developing system followed by densitometric measurements at 235 nm. The studied components were quantified in the range of 0.3-4, 0.3-3, and 1.5-5 μg/ band, respectively [62].…”

Section: -Tlc Densitometrymentioning

confidence: 99%

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Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

et al. 2020

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Background: The heterocyclic compounds are extremely important with wide array of synthetic, pharmaceutical, and industrial applications. Heterocyclic-containing compounds have been reported for their broad spectrum of biological activities including antibacterial, antifungal, antiviral, antiprotozoal, and anthelmintic activity. Main text: Several techniques have been used for the quantitation of heterocyclic compounds in pharmaceutical samples such as high-performance liquid chromatography (HPLC) either equipped with UV-visible or fluorescence, in addition to liquid chromatography-mass spectroscopy, UV-visible spectrophotometry, and electrochemical techniques. This article reviewed several published methods that have been applied to detect and quantify some pharmaceutical drugs containing heterocyclic compounds focusing on four drugs: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol. Conclusion: From literature reviews, HPLC is the most widely used analytical technique for the quantitative analysis of the four selected drugs.

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Section: -High-performance Liquid Chromatography Technique (Hplc)mentioning

confidence: 99%

Section: -High-performance Liquid Chromatography Technique (Hplc)mentioning

confidence: 99%

Section: -Tlc Densitometrymentioning

confidence: 99%

Section: -Tlc Densitometrymentioning

confidence: 99%

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Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

et al. 2020

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“…Hydrocortisone, clioquinol and their binary dosage form are official in British Pharmacopoeia [5] and USP [6]. Several methods for the determination of the drugs have been reported in different dosage forms [7][8][9][10][11][12][13]. Literature survey revealed that HCA and CL binary mixture was determined by HPLC and TLC methods [14,15].…”

Section: Introductionmentioning

confidence: 99%

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

et al. 2021

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Simple and precise spectrophotometric methods for quantitative assay of a mixture of hydrocortisone acetate (HCA) and clioquinol (CL) were developed and validated through different mathematical manipulation pathways. The developed methods utilized ratio spectra for resolving binary mixtures including absorbance subtraction, ratio subtraction coupled with spectrum subtraction, constant multiplication, constant value, and derivative ratio. The proposed methods were proved to be specific by analysing the laboratory-prepared mixtures and were applied for the assay of topical preparation successfully. The methods were validated using ICH guidelines where accuracy, repeatability and intermediate precision were within the acceptable limits. The linearity range was found to be 2-22 for HCA and 1.5-7 µg/mL for CL in all proposed methods and 2-7 µg/mL for HCA and CL in absorbance subtraction method through using a unified regression equation. The findings were statistically evaluated with respect to the official and reported methods, demonstrating that there was no significant difference.

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Coupling of liquid-liquid extraction and mathematical filtration techniques for the separation and quantification of five components in semisolid dosage form with severely overlapped spectra

Lotfy

Fahmy

2020

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Two validated liquid chromatographic methods for the simultaneous determination of flumethasone pivalate, its related substance (flumethasone), and clioquinol

Cited by 8 publications

References 7 publications

Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

Review on analytical studies of some pharmaceutical compounds containing heterocyclic rings: brinzolamide, timolol maleate, flumethasone pivalate, and clioquinol

Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

Coupling of liquid-liquid extraction and mathematical filtration techniques for the separation and quantification of five components in semisolid dosage form with severely overlapped spectra

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