Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

Abd, Ahmed M.; Michael, Adel Magdy; Hassan, Said A.; Abbas, Samah S.

doi:10.5155/eurjchem.12.3.265-272.2093

Cited by 4 publications

(2 citation statements)

References 16 publications

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“…Figure 2. The chemical structure of hydrocortisone Some spectrophotometric methods have been reported to determine ciprofloxacin [9][10][11][12][13][14][15][16] and hydrocortisone [17][18][19][20][21][22][23]. Due to their intrinsic simplicity, sufficient sensitivity, affordability, and widespread presence in all quality control laboratories, these procedures are the most practical ones.…”

Section: Simultaneous Quantitative Determination Of Ciprofloxacin And...mentioning

confidence: 99%

Simultaneous Quantitative Determination of Ciprofloxacin and Hydrocortisone by H-Point Standard Addition Method

Khalil Mahmood,

Takleef Dheyab Sallal,

Hassan Mohammed Liaibi

et al. 2024

IHJPAS

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Ciprofloxacin (Cip) and hydrocortisone (Hyd) were simultaneously measured as hydrochloride and sodium succinate, respectively, using the H-point standard addition method (HPSAM). The approach can precisely identify Cip in the presence of Hyd with various analyte-to-interference ratios (5:5, 5:10, 10:5, 10:10) µg.mL-1, in mixed samples containing (1-5µg.ml-1) of Cip, at the wavelengths of (236 and 257) nm. In the same way, Hyd was analyzed in the presence of Cip in different analytes with an interference ratio of (5:5, 5:10, 10:5, 10:10) µg.mL-1, in mixed samples containing (1-5 µg.mL-1) of Hyd, at wavelengths of (266 and 278) nm. The satisfactory results show good reproducibility of the developed method (RSD equals 0.9735-1.6825 and 0.9692-1.7671 for Cip and Hyd, respectively). The results also show that the excipients had no influence on the assaying of the above drugs (Recovery, 98.87–101.73). The recommended technique has successfully been used to determine the Cip and Hyd in pharmaceutical composites simultaneously with an RSD range of (0.972 to 1.671) and (0.898 to 1.820) for Cip and Hyd, respectively.

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Section: Simultaneous Quantitative Determination Of Ciprofloxacin And...mentioning

confidence: 99%

Simultaneous Quantitative Determination of Ciprofloxacin and Hydrocortisone by H-Point Standard Addition Method

Khalil Mahmood,

Takleef Dheyab Sallal,

Hassan Mohammed Liaibi

et al. 2024

IHJPAS

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“…Several techniques can be used for elimination of interferences in spectroscopy such as mathematical manipulations [ 1 – 3 ], chemometrics [ 4 – 6 ] and signal processing. The most commonly used techniques are signal processing ones, of which the most popular is Derivative Spectrophotometry (DS).…”

Section: Introductionmentioning

confidence: 99%

Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products

Hassan,

Fekry,

Fayez

et al. 2023

BMC Chemistry

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The presence of minor components represents a challenging problem in spectrophotometric analysis of pharmaceuticals. If one component has a low absorptivity or present in a low concentration compared to the other components, this will hinder its quantitation by spectrophotometric methods. Continuous Wavelet Transform (CWT) as a signal processing technique was utilized to figure out a solution to such a problem. A comparative study was established between traditional derivative spectrophotometry (Numerical Differentiation, ND) and CWT to indicate the advantages and limitations of each technique and possibility of solving the problem of minor components. A mixture of ibuprofen (IBU) and phenylephrine (PHE) with its degradation products forming a ternary mixture was used for comparing the two techniques. The two techniques were applied on raw spectral data and on ratio spectra data resulting in four methods, namely ND, CWT, Derivative Ratio-Zero Crossing (DRZC) and Continuous Wavelet Transform Ratio-Zero Crossing (CWTR-ZC) methods. By comparing the results in laboratory prepared mixtures, CWT technique showed advantages in analysis of mixtures with minor components than ND. The proposed methods were validated according to the ICH guideline Q2(R1), where their linearity was established with correlation coefficient ranging from 0.9995 to 0.9999. The linearity was in the range 3–40 μg/mL for PHE in all methods, while for IBU it was 20–180 and 30–180 μg/mL in CWT and ND methods, respectively. The CWT methods were applied for quantitative determination of the drugs in their dosage form showing the ability of the methods to quantitate minor components in pharmaceutical formulations.

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Advanced chemometric methods as powerful tools for impurity profiling of drug substances and drug products: Application on bisoprolol and perindopril binary mixture

Hassan

Nashat

Elghobashy

et al. 2022

Spectrochimica Acta Part A: Molecular and Biomolecular Spectros

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Different spectrophotometric methods manipulating ratio spectra for the assay of hydrocortisone acetate and clioquinol in their topical preparation

Cited by 4 publications

References 16 publications

Simultaneous Quantitative Determination of Ciprofloxacin and Hydrocortisone by H-Point Standard Addition Method

Simultaneous Quantitative Determination of Ciprofloxacin and Hydrocortisone by H-Point Standard Addition Method

Continuous wavelet transform for solving the problem of minor components in quantitation of pharmaceuticals: a case study on the mixture of ibuprofen and phenylephrine with its degradation products

Advanced chemometric methods as powerful tools for impurity profiling of drug substances and drug products: Application on bisoprolol and perindopril binary mixture

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