Two novel psychrotolerant species, Bacillus psychrotolerans sp. nov. and Bacillus psychrodurans sp. nov., which contain ornithine in their cell walls

Abstract

Eleven psychrotolerant Bacillus strains with ornithine as diamino acid in position 3 of the peptide side chain of the cell wall and a G+C range of 35.7-38.4 mol% were characterized taxonomically. DNA-DNA hybridization studies confirmed previously physiologically established groups. High DNA-binding values (> 70%) were found within groups I A (consisting of the type strain of Bacillus insolitus DSM 5(T) and Bacillus insolitus DSM 2272), I B (consisting of isolates 3H1(T0, 71H1, 84E1, 87H2 and 4H2) and I C (consisting of isolates 68E39T), 61E1, 4E3 and 67E1). Low DNA-binding values (< 60%) were revealed between the three groups. Consequently, strains of groups I B and I C were considered as being representatives of new psychrotolerant species. For group I B strains the name Bacillus psychrotolerans sp. nov. is proposed with the type strain 3H1(T) (= DSM 11706(T) = NCIMB 13838(T)) and for group I C strains the name Bacillus psychrodurans sp. nov. is proposed with the type strain 68E3(T) (= DSM 11713(T) = NCIMB 13837(T)).

Lysinibacillus macroides sp. nov., nom. rev.

“…Despite the differences in peptidoglycan type between V. arenosi , V. arvi and V. neidei , all three species have been assembled within a single genus, Viridibacillus . This is in concordance with Abd El-Rahman et al (2002), who did not wish to propose a new genus to accommodate Bacillus psychrodurans , Bacillus psychrotolerans and Bacillus insolitus based only upon the distinctive A4β l -Orn– d -Glu type alone. They reasoned that such single-parameter-based taxa are prone to be unstable and that such a proposal would be premature.…”

Bacillus beringensis sp. nov., a psychrotolerant bacterium isolated from the Bering Sea

“…This large taxonomic group contains many different physiological types. Among these, ten psychrotolerant or psychrophilic Bacillus species have been described to date: Bacillus cecembensis (Reddy et al 2008), Bacillus globisporus (Larkin and Stokes 1967;Rüger and Richter 1979;Nakamura 1984), Bacillus insolitus (Larkin and Stokes 1967), Bacillus macquariensis (Marshall and Ohye 1966), Bacillus marinus (Rüger 1983;Rüger et al 2000), Bacillus psychrodurans (Abd EI-Rahman et al 2002), Bacillus psychrophilus (Rüger and Richter 1979;Larkin and Stokes 1967;Nakamura 1984), Bacillus psychrotolerans (Abd El-Rahman et al 2002), Bacillus psychrosaccharolyticus (Larkin and Stokes 1967;Priest et al 1988) and Bacillus weihenstephanensis (Lechner et al 1998). However, four psychrotolerant or psychrophilic Bacillus species have also been reclassified as members of other genera: B. macquariensis has been transferred to the genus Paenibacillus (Ash et al 1993); B. marinus has been reclassified as Marinibacillus marinus (Yoon et al 2001b); B. globisporus and B. psychrophilus have been transferred to the genus Sporosarcina (Yoon et al 2001a).…”

Isolation and characterisation of H2O2-decomposing bacteria from Antarctic seawater

“…It was likely that the Antarctic seawater isolates belonged to the same species. Some psychrophilic species of Bacillus could grow at 35 or 40 °C (AbdEl-Rahman et al, 2002).…”