Eleven psychrotolerant Bacillus strains with ornithine as diamino acid in position 3 of the peptide side chain of the cell wall and a G+C range of 35.7-38.4 mol% were characterized taxonomically. DNA-DNA hybridization studies confirmed previously physiologically established groups. High DNA-binding values (> 70%) were found within groups I A (consisting of the type strain of Bacillus insolitus DSM 5(T) and Bacillus insolitus DSM 2272), I B (consisting of isolates 3H1(T0, 71H1, 84E1, 87H2 and 4H2) and I C (consisting of isolates 68E39T), 61E1, 4E3 and 67E1). Low DNA-binding values (< 60%) were revealed between the three groups. Consequently, strains of groups I B and I C were considered as being representatives of new psychrotolerant species. For group I B strains the name Bacillus psychrotolerans sp. nov. is proposed with the type strain 3H1(T) (= DSM 11706(T) = NCIMB 13838(T)) and for group I C strains the name Bacillus psychrodurans sp. nov. is proposed with the type strain 68E3(T) (= DSM 11713(T) = NCIMB 13837(T)).
The present study, lab and field work was conducted to evaluation of toxic-effect and bio-activity of some newly conventional-pesticides (challenger, oberon, lambda, lant, neem oil and eucalyptus oil) to control of females-adult and stages of eggs for two spotted spider mite T. urticae. Addition to, evaluation of side-effects of sub-lethal dose of tested-chemicals on some biology aspects of spider mite T. urticae, and evaluates of compounds toxicity tested pest invested cotton-plants in fields. In laboratory and field the results showed that, challenger gave high toxicity to eggs-stages and adult-females of tested pest. The result indicated that, eucalyptus oil and neem oil were the least compound. Challenger and oberon are the most effective compounds tested on egg deposition and the same effective challenger and oberon were the most effective compounds against the motile stages, while lant was of moderate effect of T. urticae. Eucalyptus oil and neem-oil gave lower effects to motile-stages of the tested pest. Challenger and oberon exhibited the best results in this respect. All compounds successes in population-density reduction of motile-stages of spider mite, T. urticae in lab and field-experiments during the two seasons 2019-2020.
Laboratory and field experiments were carried out to evaluate the toxic effect and also the bioactivity of certain recently conventional pesticides (acarmite nano, acarmite common, Spirodiclofen nano, Spirodiclofen common, eucalypts extract nano eucalypts extract common, boletus satanas nano and boletus satanas common) against females adult and eggs for two spotted "spider mite" Tetranychus urticae, Koch. Moreover, to evaluate the sub lethal doses side effects of compounds tested on several bio-aspects of T. urticae, and evaluates of these toxicological actions of these compounds aginst tested pest T. urticae, on infested cottons under the field condition. Data obtained cleared that, the acarmite nanoparticles was the biggest toxic against females adult of T. urticae followed by common solution of acarmite.While nanoparticles of each eucalypts extract and boletus satanas give intermediate toxic to tested femoles of T.urticae .Common solution of eucalypts extract and boletus satanas were lowest action of toxic against T.urticae females.
Aphids, particularly Aphis gossypii Glov., is one of the most-important-pests in squash farms, caused serious losses by sucking plant sap in addition to plant viruses' transmission such as Papaya ringspot virus-W (PRSV-W). The current work conducted to study of the effect of various-systemic-insecticides and mineral-oil against melon-aphid, "Aphis gossypii" and its ability on transmissions of non-persistent-viruses under laboratory conditions. Data obtained showed that the "flonicamid" and "pymetrozine" recorded significantly-reduction in virus-acquisition and inoculations when compared-with control "also" reductions were compared-with mineral-oil applications. On-other-hand, data obtained of insecticide-residues activity and KZ-oil on "PRSV" transmission-rate by "A. gossypii" revealed that flonicamid had significantly higher persistence up to 12 days followed by pymetrozine. The mineral oil experienced a significant increase in transmission rate as its efficacy decreased dramatically on the eighth transmission and twelfth days.
scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.