Two novel peptides from ark shell protein stimulate osteoblast differentiation and rescue ovariectomy-induced bone loss

Ark shell protein‐derived bioactive peptides promote osteoblastic differentiation through upregulation of the canonical Wnt/β‐catenin signaling in human bone marrow‐derived mesenchymal stem cells

“…In our previous study, we demonstrated that AWLNH and PHDL stimulated the phosphorylation of p38, JNK, and ERK, leading to the activation of BMP that triggered the signaling cascade through the canonical Smad‐dependent pathway in osteoblast differentiation of mBMMSCs. These findings indicated that the MAPK pathway plays a pivotal role in bone formation in mBMMSCs (Oh, Ahn, Hyung et al, 2019). In osteoblast differentiation, a cooperative regulation was found between BMP signaling pathway and Wnt/β‐catenin signaling pathway (Zhang et al., 2013).…”

“…Recently, our group demonstrated that low MW BAPs from seafood proteins promoted osteoblast differentiation of mouse bone marrow‐derived mesenchymal stem cells (mBMMSCs) through activating BMP‐signaling pathway (Hyung, Ahn, & Je, 2016, 2018). In our previous study, we identified two BAPs (AWLNH and PHDL) from ark shell protein hydrolysates and found that two BAPs stimulated ERK‐ and JNK‐dependent BMP signaling pathway during osteoblast differentiation of mBMMSCs (Oh, Ahn, Hyung & Je, 2019). AWLNH and PHDL were also shown to reduce bone turnover in OVX‐mice.…”

Effects of compound deer bone extract on osteoporosis model mice and intestinal microflora

“…Bilateral ovariectomy in female mice is a mature method to establish an animal osteoporosis model induced by estrogen deficiency (Oh et al, 2019). E2, as the main component of estrogen, acts on osteoblast precursor cells and osteoclasts by binding to estrogen receptor α to reduce the activity of proto‐oncogene c‐jun and thus inhibit the osteoclast differentiation induced by osteoclast differentiation factor RANKL, leading to the apoptosis of osteoclasts; meanwhile, E2 can also promote osteoblasts to synthesize and secrete osteoprotegerin (OPG), and OPG competes with RANKL to bind to osteoclast precursor cells, thereby inhibiting the differentiation and proliferation of osteoclasts (Lee et al, 2019).…”

Anti‐inflammatory action of ark shell ( Scapharca subcrenata ) protein hydrolysate in LPS‐stimulated RAW264.7 murine macrophages

“…S. subscrenata, an ark shell, is a popular edible seafood in Korea, Japan, and China and inhabit in the coastal water which consists of muddy sediments (Nakamura, 2005). Recently, our group demonstrated that ark shell protein hydrolysates exhibited anti-adipogenic, antioxidant, osteogenic, and anti-osteoporotic effects (Hyung et al, 2017;Jin et al, 2018;Oh et al, 2019Oh et al, , 2020. However, anti-inflammatory properties of ark shell protein hydrolysates have not been investigated yet.…”