Effects of compound deer bone extract on osteoporosis model mice and intestinal microflora

Xue, Chuang; Wang, Pan; Lu, Xue-Chun; Guo, Jiang; Xu, Guangyu; Yu, Shengquan; Gao, Yuan; Zhao, Nanxi; Sun, Jingbo; Guo, Xiaoyi; Wang, Manli; Li, Hongyu; An, Lihui; Han, Xiao

doi:10.1111/jfbc.13740

Cited by 11 publications

(11 citation statements)

References 31 publications

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“…The SMP is a parameter describing the proportion of the plate and rod structures in the constituent structures of trabecular architecture. 64 The Female MD group showed a 63.14% decrease in Tb•Pf and a 36.32% decrease in the SMI compared to the female LC group. In addition, there was no significant difference between the MD, PC and NC groups ( p > 0.05).…”

Section: Quantitative Parameters Of Cancellous Bone Micro-ct Of the F...mentioning

confidence: 83%

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

et al. 2023

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Section: Quantitative Parameters Of Cancellous Bone Micro-ct Of the F...mentioning

confidence: 83%

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

et al. 2023

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Section: Discussionmentioning

confidence: 99%

“…Various plant-derived compounds have been reported to promote osteogenesis, osteoid formation, hydroxyapatite synthesis, and mineral apposition via multiple signaling pathways and transcription factors [ 5 , 6 , 15 , 16 ]. All of these studies provided novel drug research, development, and clinical applications for treating bone diseases [ 15 , 16 , 17 , 18 , 19 , 20 , 21 ]. The present study is the first to investigate the osteogenic effects of TriFs isolated from Gentianae Scabrae Radix on osteoblast differentiation, maturation, and function via intracellular signals and the RUNX2 transcription factor without noticeable autophagic flux and necroptosis.…”

Section: Discussionmentioning

confidence: 99%

Trifloroside Induces Bioactive Effects on Differentiation, Adhesion, Migration, and Mineralization in Pre-Osteoblast MC3T3E-1 Cells

Yun

Kim

Park

et al. 2022

Cells

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Gentianae Scabrae Radix is used in traditional medicine and is known to possess bioactive compounds, including secoiridoid glycosides, flavonoids, lignans, and triterpenes. Trifloroside (TriFs) is a secoiridoid glycoside known for its antioxidant activity; however, its other effects have not been studied. In the present study, we investigated the biological effects of TriFs isolated from the roots of Gentianae Scabrae Radix using pre-osteoblast MC3T3E-1 cells. No cellular toxicity was observed with 1 mM TriFs, whereas 5–100 mM TriFs showed a gradual increase in cell viability. Alkaline phosphatase staining and microscopic observations revealed that 1–10 mM TriFs stimulated osteogenic activity during early osteoblast differentiation. Trifloroside also increased mineral apposition during osteoblast maturation. Biochemical analyses revealed that TriFs promoted nuclear RUNX2 expression and localization by stimulating the major osteogenic BMP2-Smad1/5/8-RUNX2 pathway. Trifloroside also increased p-GSK3b, β-catenin, p-JNK, and p-p38, but not Wnt3a, p-AKT, and p-ERK. Moreover, TriFs increased the MMP13 levels and promoted cell migration and adhesion. In contrast, TriFs-induced osteoblast differentiation and maturation had negligible effects on autophagy and necrosis. Our findings suggest that TriFs induces osteogenic effects through differentiation, adhesion, migration, and mineral apposition. Therefore, TriFs is suggested as a potential drug target in osteoblast-mediated bone diseases.

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“…An et al gave glucocorticoid-induced osteoporosis rats gavage of deer bone polypeptide, and the results showed that deer bone polypeptide can inhibit the imbalance of calcium and phosphorus metabolism induced by dexamethasone, reduce alkaline phosphatase (ALP), increase osteocalcin, inhibit bone resorption and promote bone formation, and improve the pathological changes and microstructure of bone in osteoporotic rats (Li-ping, 2016). In addition, several preclinical studies have shown that deer bone-related products, such as deer BM and deer melon polypeptide, can effectively relieve osteoporosis caused by ovariectomy in rats and promote osteoblast proliferation (He, 2011;Xue et al, 2021).…”

Section: Introductionmentioning

confidence: 99%

Physical and Chemical Properties, Biosafety Evaluation, and Effects of Nano Natural Deer Bone Meal on Bone Marrow Mesenchymal Stem Cells

Tan

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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At present, bone-based products are abundant, and the main sources are bovine bone and pig bone, but there are few studies on the development of deer bone as a bone repair material. Deer bone has important osteogenic effects in the theory of traditional Chinese medicine. It is rich in protein, ossein, and a variety of trace elements, with the effect of strengthening tendons and bones. Nanomaterials and their application in the repair of bone defects have become a research hotspot in bone tissue engineering. In this study, nano-deer bone meal (nBM), nano-calcined deer bone meal, and nano-demineralized bone matrix were successfully prepared. It was found that the Ca/P ratio in deer bone was significantly higher than that in cow bone and human bone tissue, and deer bone contained beneficial trace elements, such as potassium, iron, selenium, and zinc, which were not found in cow bone. The three kinds of deer bone powders prepared in this study had good biocompatibility and met the implantation standards of medical biomaterials. Cell function studies showed that compared with other bone powders, due to the presence of organic active ingredients and inorganic calcium and phosphate salts, nBM had excellent performance in the proliferation, adhesion, migration, and differentiation of bone marrow mesenchymal stem cells. These findings indicate that nBM can be used as a potential osteoinductive active nanomaterial to enhance bone tissue engineering scaffolds with certain application prospects.

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Effects of compound deer bone extract on osteoporosis model mice and intestinal microflora

Cited by 11 publications

References 31 publications

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

Preparation of synbiotic milk powder and its effect on calcium absorption and the bone microstructure in calcium deficient mice

Trifloroside Induces Bioactive Effects on Differentiation, Adhesion, Migration, and Mineralization in Pre-Osteoblast MC3T3E-1 Cells

Physical and Chemical Properties, Biosafety Evaluation, and Effects of Nano Natural Deer Bone Meal on Bone Marrow Mesenchymal Stem Cells

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